Five specimens of the genus Crocidura were collected from the Moxinjian Provincial Nature Reserve of Chun’an city in Zhejiang Province and from the Dongjiangyuan Provincial Nature Reserve of Dongyang city in August 2017. Phylogenetic reconstruction based on the mitochondrial cytochrome b gene (cyt b) revealed that the unidentified specimens collected from Zhejiang Province forms a distinct clade, which is close to C. lasiura and C. dsinezumi. The genetic distances among them range from 0.101-0.155. Large genetic distances were identified between the unidentified specimens and species from southern China, C. indochinensis and C. wuchihensis, and species from the northeast, C. shantungensis and C. suaveolens. K2P distances among the unidentified species and C. indochinensis, C. wuchihensis, C. shantungensis, C. suaveolens were 0.114, 0.124, 0.139 and 0.144, respectively. The diagnostic characteristics of the unidentified genetic lineage are as follows: body small, head-body 48-61 mm(mean: 55.60 mm), tail length 35-49 mm (mean: 43.14 mm); and the tail is relatively short (72.92%-80.36% of head-body length), almost bare, and long sparse hairs are present only along the basal third to the second of the tail, with a short terminal pencil. Tail is bicolor, black brownish above, and paler beneath. Skull is shorter，Condylo-incisive length 15.60-17.16 mm (mean: 16.46 mm). Results from the combined molecular and morphological analyses indicated that the undefined specimens should be a new species, Crocidura dongyangjiangensis Liu Y, Chen SD, and Liu SY, sp. nov.. Detailed description of this new species is given in the present study.