Two specimens of Mesechinus hughi were collected from Pingshan, Yi County, Huangshan (29°56'23"N, 117°58'48"E) and Xikou, Xuanzhou, Xuancheng (30°34'42"N, 118°41'47"E) in Anhui Province, in July and September 2018, respectively. Ears of the two specimens are similar, in length, to the surrounding spines. The two specimens have few wholly white spines, and most spines (>95%) have four sections; white constituting the basal two-thirds, followed by a black ring, narrow light ring, and black tip. These characters are similar to those of the specimens from the type locality (Baoji, Shaanxi Province). The P-distance of Cyt b between the population in Anhui and that of Shaanxi is from 4.9% to 5.3%, and maximum likelihood phylogenetic analyses support the two populations as monophyletic (BS = 95%). This was the first recorded distribution of Mesechinus in Anhui Province and Mesechinus hughi south of the Changjiang River.