For captive endangered species, variations of blood chemistry parameters are useful for the diagnosis and treatment of disease. From April 2018 to January 2019, blood samples from 35 captive chimpanzees (Pan troglodytes) from 14 zoos in China were collected, and 9 blood physiological parameters and 13 biochemical parameters were measured. Blood parameters between sexes and among age classes were compared. The results showed no significant sex difference in blood chemistry (P>0.05), except WBC, UREA and PHOS (P<0.05), in captive chimpanzees. PLT and ALKP concentrations decrease and CREA increases with age. The variations of values among different sex/age demonstrated by this study would benefit the diagnosis and treatment of disease in captive chimpanzees.