During July-August 2017 and June-July 2018, we studied the relationships between stereotyped behavioral development and fecal steroid hormone (cortisol, testosterone and estradiol) in captive Alpine musk deer. The research took place at the Musk Deer Breeding Center of Xinglong Shan Nature Reserve, in Gansu Province. We collected stereotypic behavior data using focal sampling and all-occurrence recording, while radioimmunoassay was used for fecal steroid hormone (cortisol, testosterone and estradiol) determination. We found cortisol level to be higher in Alpine musk deer with stereotyped behavior (111.099 ± 16.231) ng/g than in Alpine musk deer without stereotyped behavior (95.640 ± 9.738) ng/g (P > 0.05). There was no difference in testosterone level between male Alpine musk deer with (135.900 ± 21.582) ng/g or without (108.182 ± 9.689) ng/gstereotyped behavior (P > 0.05). However, there was significantly less estradiol in female Alpine musk deer which showed stereotyped behavior (0.445 ± 0.116) ng/g than in females not showing stereotyped behavior (10.843 ± 1.142)ng/g (P < 0.05). Finally, we found the relationship between expression of stereotyped behavior and hormone level to be sex-specific, showing a negative correlation in females but no correlation in males Alpine musk deer, which might be related to females’ reproduction and health. In musk deer farming, fecal steroid hormone levels (especially estradiol) can be considered an appropriate monitoring index reflecting the stress and health of captive musk deer.