In order to explore the influence of season, age and gender on the physiological and blood indices of captive giant panda, the blood indices of 18 individuals, including sub-adult, adult and aged, were monitored dynamically. The results showed that WBC, NEU, LYM, MON, BAS, RBC, HGB, HCT, MCV, MCHC, PLT and MPV were significantly affected by the three factors(P＜0.05),among which the interaction of age and season, gender and season had a significant effect on MON(P＜0.01).In terms of blood biochemical indices, there were significant effects on TBIL, IDBIL, TP, ALB, GLO, A/G, AST, ALT, ST/LT, GGT, ALP, LDH, PA, ADA, 5-NT, AFU, TBA, BUN, UA, BMG, Na, CL, TG, HDL, APOA1, APOB, GLU(P＜0.05).The interaction of age and season had significant effects on PA(P＜0.05),and the interaction of gender and season had significant effects on PA and BMG sound(P＜0.01).In addition, the content of Zn, Ca, Fe in blood trace elements was significantly affected by season and age(P＜0.05).In conclusion, in addition to gender and age factors, seasonal changes have a significant influence on physiological and blood indices of giant panda .