Midday gerbil (Meriones meridianus) and Northern three toed Jerboa (Dipus sagitta) are two dominant sympatric species in Alashan desert. Studying their activity rhythms is important for understanding the coexistence mechanism of desert rodents. This study was conducted in the Alashan desert of Inner Mongolian, on April (spring), July (summer), September (autumn) and October(pre-winter), 2017. We monitored M. meridianus and D. sagitta using camera-traps in order to understand the active rhythms in different seasons and explore coexistence strategies of two species. The results showed that: (1) The population of M. meridianus was restrained by the D. sagitta population density. An obviously staggered phenomenon was found between the two species peak activity. Affected by the light intensity, the activity time of two species was positively correlated with night length. The activities start time of the two species was positively correlated with the sunset and ambient temperature. (2) There was no significant season difference in activity intensity of M. meridianus. However, the D. sagitta showed the opposite strategy (F =3.202；P =0.032); (3) These result indicated that coexisting desert rodents adjusted their night time utilization patterns to reduce competition and achieve coexistence.