Long call is a common type of sound uttered by plateau pika( Ochotona curzoniae). It is important to study the temporal distribution of plateau pikas’ long call for a whole year and its influencing factors for better understanding the survival strategies of plateau pika. In this study, we used a digital recorder and video camera to record the duration of the long call by plateau pika and investigated the potential influence factors related to long call, involving temperature, wind speed, testosterone and progesterone between February 2018 and February 2019 in Maqu County, located in the eastern Tibet Plateau. The results show: (1) The long call was uttered by male plateau pika and mainly occurred in the breeding period from March to May, which was significantly higher than in other months. The duration of long call ranges in each individual male was 130-180 seconds per day and mainly uttered 2 hours after sunrise and 2 hours before sunset throughout the year. (2) The occurrence of long call uttered by male plateau pika throughout the year is closely related to pikas’ testosterone levels. When the testosterone level was about 40 pg/mL, the duration of long call was 1000 seconds, and when the testosterone level was below 36 pg/mL, the duration of long call was around 150 seconds. The long call in plateau pikas during the non-breeding period was negatively correlated with air temperature and wind speed. In conclusion, the long call uttered by male plateau pika has a typical seasonal pattern and the occurrence of long call was closely related to the testosterone levels increasing of male plateau pika during the breeding season.