ORIGINAL PAPERS

Cloning of AQP1 and AQP3 genes and their locations and expressions in different tissues of yak(Bos grunniens)

  • LI Juan ,
  • WANG Li ,
  • LUO Xiaolin ,
  • GUAN Jiuqiang ,
  • ZHANG Xiangfei
Expand
  • 1 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Vtilization, Ministry of Education and Sichuar Province, Southwest Minzu University, Chengdu 610041, China;
    2 Sichuan Academy of Grassland Sciences, Chengdu 611731, China

Received date: 2020-04-24

  Online published: 2021-01-21

Abstract

Aquaporin(AQP) is widely expressed in tissues of organisms and affects the process of water metabolism.In order to investigate the biological function of AQP1 and AQP3, the expression patterns and location of AQP1 and AQP3 in different yak tissues were clarified in this study.The PCR was used to amplify the AQP1 and AQP3 genes of yak, and their sequences were analyzed by bioinformatic techniques.The qPCR was used to detect the expression of AQP1 and AQP3 in different tissues of yak.Immunohistochemistry was used to study the expression and distribution of AQP1 and AQP3 proteins in tissues.The results showed that the CDS of AQP1 and AQP3 genes of yak were 816 bp and 840 bp.And the homology with the wild yak was at most 99% of AQP1 and AQP3.The expression levels of AQP1 and AQP3 genes were the highest in the kidney, which were significantly higher than those of the intestine, muscle, rumen and other tissues(P<0.01).The expression of AQP1 gene is higher than AQP3 in all tissues.AQP1 and AQP3 proteins were mainly distributed in the renal proximal tubules, intestinal lamina propria cells and rumen granulosa cells, and both showed the highest expression in the kidney.The results of this study have implications for the study of mammals in the alpine and hypoxic environment, and contribute to the study of the functions of AQP1 and AQP3 in yak.

Cite this article

LI Juan , WANG Li , LUO Xiaolin , GUAN Jiuqiang , ZHANG Xiangfei . Cloning of AQP1 and AQP3 genes and their locations and expressions in different tissues of yak(Bos grunniens)[J]. ACTA THERIOLOGICA SINICA, 2021 , 41(1) : 51 -58 . DOI: 10.16829/j.slxb.150433

References

Chen G,Shi Y,Liu M,Sun J.2018.circHIPK3 regulates cell proliferation and migration by sponging miR-124 and regulating AQP3 expression in hepatocellular carcinoma. Cell Death and Disease,9(2):175.
Chen L,Li Z,Zhang Q,Wei S,Li B,Zhang X,Xu Z.2017.Silencing of AQP3 induces apoptosis of gastric cancer cells via downregulation of glycerol intake and downstream inhibition of lipogenesis and autophagy.OncoTargets and Therapy,10:2791-2804.
Chen M,Li Y,Xiao L,Dai G,Lu P,Wang Y,Rui Y.2020.AQP1 modulates tendon stem/progenitor cells senescence during tendon aging. Cell Death and Disease,11(3):1-12.
Ding Y P,Yu H S,Wang J,Shao B.2019.Immunoexpression of aquaporins 1,2,3 and 4 in kidney of yak(Bos grunniens) on the Qinghai-Tibetan Plateau.Biotechnic Histochemistry,94(1):48-52.
Gan Y D.2014.DNA cloning of aquaporin3 in Sarotherodon melanothern,Oreochromis niloticus and tissue expression patterns under salinity stresses.Master thesis.Shanghai:Shanghai Ocean University.(in Chinese)
Hoogewijs D,Vogler M,Zwenger E,Krull S,Zieseniss A.2016.Oxygen-dependent regulation of aquaporin-3 expression.Hypoxia,21(4):91-97.
He J,Yang B.2019.Aquaporins in renal diseases.International Journal of Molecular Sciences,20(2):366.
Han Y H,Wang Y,Jia J J,Wang H L,Zhu J H,Lai L H,Li Y.2018.Changes of expressions of myocardial AQP1 and HIF-1α and relationship between them and myocardial edema in rats with myocardial infarction.Chinese Journal of Evidence-based Cardiovascular Medicine,10(4):418-421.(in Chinese)
Ishibashi K,Kondo S,Hara S,Morishita Y.2011.The evolutionary aspects of aquaporin family.American Journal of Physiology-regulatory,Integrative and Comparative Physiology,300(3):35-50.
Jin S Y.2006.Cloning,immunolocalization and characterization of porcine aquaporin 1 water channel.Ph.D thesis.Jilin:Northeast Normal University.(in Chinese)
Knepper M A,Nielsen S.2004.Peter Agre 2003 nobel prize winner in chemistry.Journal of The American Society of Nephrology,15(4):1093-1095.
Li M T,Fu Y,Wang Q S,Li H G.2011.Preparation of porcine aqp1 polyclonal antibody and immunolocalization of AQP1 in the pig.Journal of Jilin Agricultural University,33(2):218-221,226.(in Chinese)
Li X L,Li K,Liu Y P,Du Q,Li R C.2020.Exploring the effects of gypsum fibrosum on rat model of acute soft tissue injury based on the expression of aquaporin aqp1/aqp3.Journal of Hunan University of Traditional Chinese Medicine,40(1):9-13.(in Chinese)
Liu J F.2015.Molecular mechanism of yak(Bos grunnies) AQPs resistance to high altitude cerebral edema.Master thesis.Lanzhou:Lanzhou University.(in Chinese)
Liu M,Liu Q,Pei Y,Gong M,Cui X,Pan J,Yuan Y.2019.Aqp-1 gene knockout attenuates hypoxic pulmonary hypertension of mice.Arteriosclerosis,Thrombosis,and Vascular Biology,39(1):48-62.
Lu J C.2019.Expression and correlation of AQP1 and TRAP1 in human glioma.Master thesis.Zhengzhou:Zhengzhou University.(in Chinese)
Ma T,Song Y,Yang B,Gillespie A,Carlson E J,Epstein C J,Verkman A S.2000.Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels.Proceedings of the National Academy of Sciences of the United States of America,97(8):4386-4391.
Marlar S,Jensen H H,Login F H,Nejsum L N.2017.Aquaporin-3 in Cancer.International Journal of Molecular Sciences,18(10):2106.
Roudier N,Ripoche P,Gane P,Pennec P Y,Daniels G,Cartron J,Bailly P.2002.AQP3 deficiency in humans and the molecular basis of a novel blood group system,GIL.Journal of Biological Chemistry,277(48):45854-45859.
Su W,Cao R,Zhang X,Guan Y.2020.Aquaporins in the kidney:physiology and pathophysiology.American Journal of Physiology-renal Physiology,318(1):139-203.
Sui H,Han B G,Lee J K,Walian P,Jap B K.2001.Structural basis of water-specific transport through the aqp1 water channel. Nature,414(6866):872-878.
Umenishi F,Schrier R W.2003.Hypertonicity-induced aquaporin-1(AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene.Journal of Biological Chemistry,278(18):15765-15770.
Wang J,Liu W,Zhang X,Zhang Y,Xiao H,Luo B.2019.LMP2A induces DNA methylation and expression repression of AQP3 in EBV-associated gastric carcinoma.Virology,534:87-95.
Wang Q H.2017.Functional characteristics of AQP9 in the yak brain.Master thesis.Lanzhou:Lanzhou University.(in Chinese)
Wang S,Wu T,Lin J,Tian J,Ji Y,Pei Y,Zong B.2019.FeK on 3D graphene-zeolite tandem catalyst with high efficiency and versatility in direct CO2 conversion to aromatics.ACS Sustainable Chemistry Engineering,7(21):17825-17833.
Wang X,Tao C,Yuan C,Ren J,Yang M,Ying H.2017.AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma.Molecular Medicine Reports,16(2):1964-1972.
Yusupov M,Razzokov J,Cordeiro R M,Bogaerts A.2019.Transport of reactive oxygen and nitrogen species across aquaporin:a molecular level picture.Oxidative Medicine and Cellular Longevity,2019:1-11.
Zhang X L.2017.Gene cloning and sequence analysis of AQP3 in human skin.Master thesis.Tianjin:Tianjin Medical University.(in Chinese)
Zheng Y,Zhao Q.2018.Expression of AQPl and AQP2 in renal tissue of congenital hydronephrosis and its correlation with glomerular filtration rate.International Journal of Laboratory Medicine,39(6):687-689.(in Chinese)
Zhu C,Chen Z,Jiang Z.2016.Expression distribution and role of aquaporin water channels in human and animal stomach and intestines.International Journal of Molecular Sciences,17(9):1399.
Zhu F,Tajkhorshid E,Schulten K.2001.Molecular dynamics study of aquaporin-1 water channel in a lipid bilayer.FEBS Letters,504(3):212-218.
Zhu Y K,Li Y H,Zhu G Z.2020.Research progress on the relationship between aquaporin 3 and gastrointestinal diseases. Chinese Journal of General Practice,18(1):109-112.(in Chinese)
甘远迪.2014.萨罗罗非鱼、尼罗罗非鱼AQP3 cDNA序列克隆及盐度胁迫下组织表达特征.上海:上海海洋大学硕士学位论文.
卢家潮.2019.AQP1、TRAP1在人脑胶质瘤中的表达及相关性.郑州:郑州大学硕士学位论文.
李心亮,李柯,刘月平,杜青,李若存.2020.基于水通道蛋白AQP1/AQP3的表达探讨石膏对急性软组织损伤大鼠模型的影响.湖南中医药大学学报,40(1):9-13.
朱烨柯,李玉红,祝贵州.2020.水通道蛋白3与胃肠道疾病相关性研究进展.中华全科医学,18(1):109-112.
刘健锋.2015.牦牛AQPs对高原脑水肿抗性的分子机理.兰州:兰州大学硕士学位论文.
汪迁昊.2017.牦牛脑AQP9的功能特征.兰州:兰州大学硕士学位论文.
张秀丽.2017.人皮肤AQP3基因克隆及序列分析.天津:天津医科大学硕士学位论文.
李明堂,付玉,王清爽,李洪广.2011.猪水通道蛋白AQP1多克隆抗体制备及其在猪体内的免疫定位.吉林农业大学学报,33(2):218-221,226.
郑妍,赵琦.2018.AQP1、AQP2在先天性肾积水肾组织中的表达及与肾小球滤过率的相关性研究.国际检验医学杂,39(6):687-689.
金顺英.2006.猪水通道AQP1基因分子克隆、组织定位及功能分析.长春:东北师范大学博士学位论文.
尚俊依,李尔然,王秋月,康健.2011.低氧诱导AQP1表达的分子机制.见:袁小燕.第十二次全国呼吸病学学术会议.北京:科学出版社,299-300.
韩延辉,王燕,贾静静,王红雷,朱继红,来利红,李影.2018.心肌梗死大鼠心肌组织中AQP1、HIF-1α的表达变化及其与心肌水肿的关系.中国循证心血管医学杂,10(4):418-421.
Outlines

/

〈 〉