SPECIAL SUBJECT OF ZOONOTIC DISEASES

Survey on serotypes of Salmonella isolated from mink and its pathogenesis in mice

  • ZHOU Guodong ,
  • ZHU Qian ,
  • SHI Wenjian ,
  • YUAN Peng ,
  • JIANG Shijin ,
  • XIE Zhijing
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  • College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China

Received date: 2020-08-14

  Online published: 2021-05-27

Abstract

Thirty-five (66.04%) Salmonella strains were isolated from 53 farmed mink samples collected in Zhucheng, Shandong Province, China, which were identified using selective culture, biochemical assays, and serotyping assays. Thirty-four (97.14%) of the 35 isolates were identified as Salmonella enteritidis (Se), named as Se-SD-1-Se-SD-34, followed by S. typhimurium (St) (1/35), named as St-SD-1. The 10 virulence-associated genes of Salmonella were tested using PCR. The prevalence of invA and mgtC genes for the isolates were 100%(35/35), sopE and tolC genes 97.14%(34/35), stn gene 94.29%(33/35), avrA gene 88.57%(31/35), spiA gene 82.86%(29/35), hilC gene 68.57%(24/35), spvB gene 62.86%(22/35) and pefA genes 42.86%(15/35). It demonstrated that the virulence gene contents were different among the Salmonella isolates. Antimicrobial susceptibility of 11 antimicrobials (amoxicillin etc.) for the isolates was performed using disk diffusion method. Thirty-four Se isolates exhibited multidrug resistance and cross resistance, and the highest level of resistance was found to kanamycin(15/34). The St isolate was only resistant to tetracycline. According to serotypes and virulence gene contents of the isolates, Se-SD-1 and St-SD-1 were selected for mouse experiments. As a result, Se-SD-1 and St-SD-1 caused the diseases in mice, and the inoculation doses could have influences on mouse mortality. This study enriches the research data of mink salmonellosis. It is necessary to further carry out the etiology and epidemiological surveillance of salmonella in mink for public health.

Cite this article

ZHOU Guodong , ZHU Qian , SHI Wenjian , YUAN Peng , JIANG Shijin , XIE Zhijing . Survey on serotypes of Salmonella isolated from mink and its pathogenesis in mice[J]. ACTA THERIOLOGICA SINICA, 2021 , 41(3) : 245 -253 . DOI: 10.16829/j.slxb.150468

References

Ahmed A O,Raji M A,Mamman P H,Kwanashie C N,Raufu I A,Aremu A,Akorede G J.2019.Salmonellosis:Serotypes,prevalence and multi-drug resistant profiles of Salmonella enterica in selected poultry farms,Kwara State,North Central Nigeria.The Onderstepoort Journal of Veterinary Research,86(1):e1-e8.
Akbar S,Schechter L M,Lostroh C P,Lee C A.2003.AraC/XylS family members,HilD and HilC,directly activate virulence gene expression independently of HilA in Salmonella typhimurium.Molecular Microbiology,47(3):715-728.
Bäumler A J,Tsolis R M,Heffron F.1996.Contribution of fimbrial operons to attachment to and invasion of epithelial cell lines by Salmonella typhimurium.Infection and Immunity,64(5):1862-1865.
Blanc-Potard A B,Solomon F,Kayser J,Groisman E A.1999.The SPI-3 pathogenicity island of Salmonella enterica.Journal of Bacteriology,181(3):998-1004.
Chen R,Xiong H J,Wang P Y,Dong J H,Sun Y H,Song L.2010.Cloning and DNA sequence analysis of enterotoxin gene from Salmonella.Progress in Veterinary Medicine,31(9):25-28.(in Chinese).
Dietz H H,Chriél M,Andersen T H,Jørgensen J C,Torpdahl M,Pedersen H,Pedersen K.2006.Outbreak of Salmonella dublinassociated abortion in Danish fur farms.Canadian Veterinary Journal,47(12):1201-1205.
Founou L L,Founou R C,Essack S Y.2016.Antibiotic resistance in the food chain:a developing country-perspective.Frontiers in Microbiology,7:1881.
Galán J E,Curtiss III R.1991.Distribution of the invA,-B,-C,and -D genes of Salmonella typhimurium among other Salmonella serovars:invA mutants of Salmonella typhi are deficient for entry into mammalian cells.Infection and Immunity,59(9):2901-2908.
Gangwar M,Waters A M,Bej G A,Bej A K,Mojib N.2013.Detection of Salmonella in shellfish using SYBR GreenTM I-Based Real-Time multiplexed PCR assay targeting invA and spvB.Food Analytical Methods,6:922-932.
Gorham J R,Cordy D R,Quortrup E R.1949.Salmonella infections in mink and ferrets.American Journal of Veterinary Research,10(35):183-192.
Groisman E A,Ochman H.1997.How Salmonella became a pathogen.Trends in Microbiology,5(9):343-349.
Gulig P A,Danbara H,Guiney D G,Lax A J,Norel F,Rhen M.1993.Molecular analysis of spv virulence genes of the Salmonella virulence plasmids.Molecular Microbiology,7(6):825-830.
Huehn S,La Ragione R M,Anjum M,Saunders M,Woodward M J,Bunge C,Helmuth R,Hauser E,Guerra B,Beutlich J,Brisabois A,Peters T,Svensson L,Madajczak G,Litrup E,Imre A,Herrera-Leon S,Mevius D,Newell D G,Malorny B.2010.Virulotyping and antimicrobial resistance typing of Salmonella enterica serovars relevant to human health in Europe.Foodborne Pathogens and Disease,7(5):523-357.
Hur J,Choi Y Y,Park J H,Jeon B W,Lee H S,Kim A R,Lee J H.2011.Antimicrobial resistance,virulence-associated genes,and pulsed-field gel electrophoresis profiles of Salmonella enterica subsp.enterica serovar Typhimurium isolated from piglets with diarrhea in Korea.Canadian Journal of Veterinary Research,75(1):49-56.
Jajere S M.2019.A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors,host specificity and antimicrobial resistance including multidrug resistance.Veterinary World,12(4):504-521.
Jiao Y. 2019.Role of Salmonella enteritidis gene rfbG,rfbH,and avrA and their molecular mechanisms. Ph.D thesis.Yangzhou:Yangzhou University.(in Chinese).
Mizusaki H,Takaya A,Yamamoto T,Aizawa S.2008.Signal pathway in salt-activated expression of the Salmonella pathogenicity island 1 type III secretion system in Salmonella enterica serovar Typhimurium.Journal of Bacteriology,190(13):4624-4631.
Murugkar H V,Rahman H,Dutta P K.2003.Distribution of virulence genes in Salmonella serovars isolated from man & animals.Indian Journal of Medical Research,117:66-70.
Prager R,Rabsch W,Streckel W,Voigt W,Tietze E,Tschäpe H.2003.Molecular properties of Salmonella enterica serotype paratyphi B distinguish between its systemic and its enteric pathovars.Journal of Clinical Microbiology,41(9):4270-4278.
Rahman H,Streckel W,Prager R,Tschape H.2004.Presence of sopE gene & its phenotypic expression among different serovars of Salmonella isolated from man & animals.Indian Journal of Medical Research,120(1):35-38.
Reed L J,Muench,H.1938.A simple method of estimating fifty per cent endpoints.American Journal of Epidemiology,
27(3):493-497.
Shanmugasamy M,Velayutham T,Rajeswar J.2011.Inv A gene specific PCR for detection of Salmonella from broilers.Veterinary World,4(12):562-564.
Skyberg J A,Logue C M,Nolan L K.2006.Virulence genotyping of Salmonella spp.with multiplex PCR.Avian Diseases,50(1):77-81.
Stone B J,Miller V L.1995.Salmonella enteritidis has a homologue of tolC that is required for virulence in BALB/c mice.Molecular Microbiology,17(4):701-712.
Sun N,Chen Q,Cheng Y N,Yang Y,Wang G L,Liu Z Y,Zhang S Q,Cheng S P.2019.Isolation,identification and drug sensitivity analysis of Salmonella from minks.China Animal Husbandry & Veterinary Medicine,46(2):635-641.(in Chinese).
Williams D R,Bellhouse R.1974.The prevalence of salmonellas in mink.Journal of Hygiene,72(1):71-78.
Zhao C,Zhang Q,Guo S Y,Li L L,Liu Y J,Wang Y M,Chang W S,Liu Y Q.2017.Distribution and typing of animal-derived Salmonella with MLST and serotype.Chinese Journal of Zoonoses,33(9):793-799.(in Chinese).
王光华,李玉田.2009.貂沙门氏菌病的防治.广西畜牧兽医,25(1):44-45.
孙娜,陈强,程悦宁,杨勇,王改丽,刘泽佘,张淑琴,程世鹏.2019.水貂沙门氏菌的分离鉴定及药物敏感性分析.中国畜牧兽医,46(2):635-641.
陈瑞,熊惠军,王培园,董剑辉,孙跃辉,宋立.2010.沙门菌肠毒素基因克隆及序列分析.动物医学进展,31(9):25-28.
陈溥言.2015.兽医传染病学.北京:中国农业出版社,117-125.
赵翠,张庆,郭树源,李璐璐,刘玉洁,王永明,常维山,刘玉庆.2017.山东省动物源沙门氏菌MLST和血清分型与分布研究.中国人兽共患病学报,33(9):793-799.
董文娟,吕艳艳,蔡淑霞.2015.一例水貂感染沙门氏菌的诊治.特种经济动植物,10:11-12.
焦扬.2019.肠炎沙门菌rfbG、rfbH、avrA基因的功能及其分子机制研究.扬州:扬州大学博士学位论文.
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