Genetic diversity and demographic history of Siberian flying squirrel (Pteromys volans) population in northern Zhangguangcai Mountains, Heilongjiang, China
Received date: 2021-10-30
Accepted date: 2022-02-23
Online published: 2022-07-22
The Siberian flying squirrel (Pteromys volans) is an arboreal, nocturnal, and gliding rodent. It plays an important role in forest seed dispersal and ecosystem balance maintenance. We used three molecular markers, namely mtDNA Cytb, control region, and nDNA microsatellites, to conduct genetic diversity and demographic history analyses of the flying squirrel population in the north Zhangguangcai Mountains, Heilongjiang Province. The mean haplotype diversity based on the complete sequence of Cyt b gene (1 140 bp) was 0.909 and the mean nucleotide diversity was 0.616%. The mean haplotype diversity based on the complete sequence of the control region (1 066 bp) was 0.945 and the mean haplotype diversity was 1.698%. The mean number of alleles in the population detected by 12 microsatellite loci was 13.167, the observed heterozygosity was 0.727, the expected heterozygosity was 0.864 and the inbreeding coefficient was 0.159. Our results indicated a high level of genetic diversity in the flying squirrel population, but low heterozygosity and some level of inbreeding. No evidence of population genetic bottleneck or within-population genetic differentiation was found. However, high proportion of rare haplotypes (≥ 60%), low frequency of alleles, and inbreeding indicate a risk of declining genetic diversity in the Siberian flying squirrel population in the future. It is suggested that conservation efforts forSiberian flying squirrels in this region should be improved. The phylogenetic relationship of Cyt b haplotypes confirmed that there are three major lineages of Siberian flying squirrel occupying the Far Eastern, northern Eurasia, and the island of Hokkaido. The haplotypes of Zhangguangcai and Daxing’an Mountains in this study are part of the lineage Far Eastern.
Xinmin TIAN , Mingdong LIAN , Yaqi SONG , Xiaohui LIU , Mengping YANG , Hong CHEN . Genetic diversity and demographic history of Siberian flying squirrel (Pteromys volans) population in northern Zhangguangcai Mountains, Heilongjiang, China[J]. ACTA THERIOLOGICA SINICA, 2022 , 42(4) : 398 -409 . DOI: 10.16829/j.slxb.150629
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