Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non‑breeding seasons
Received date: 2021-09-02
Accepted date: 2022-02-23
Online published: 2022-07-22
The plateau zokor (Eospalax baileyi) is an endemic subterranean rodent species with limited exposure light. Previous study has shown seasonal differences in levels of melatonin secretion in plateau zokors. However, the expression pattern of melatonin receptor genes on hypothalamic?pituitary?gonadal (HPG) axis is not clear. In this study, q–PCR was employed to detect the relative expression levels of genes mRNA of melatonin receptor 1a (MTNR1a)and melatonin receptor 1b (MTNR1b) in hypothalamus, pituitary, and testis of male plateau zokors during the breeding (May) and non?breeding seasons (September). The location of MTNR1a and MTNR1b in testis was determined by immunohistochemical technology, and immunohistochemical positive evaluation was conducted in Image Pro Plus software. The results showed that the relative expression levels of MTNR1a in hypothalamus and pituitary were significantly higher during the breeding season (P < 0.05), while there was no significant difference in the relative expression levels of MTNR1b in different seasons (P > 0.05). The relative expression levels of MTNR1a and MTNR1b in testis during the non?breeding season were higher than that during the breeding season (P < 0.01). The positive signal of MTNR1a in testis was observed in all types of cells except elongated spermatid during the breeding season and in Leydig cells, Sertoli cells, and spermatogonia during the non?breeding season. The positive signal of MTNR1b in testis was observed in all types of cells except spermatogonia and elongated spermatid during the breeding season and in Leydig cells and Sertoli cells during the non?breeding season. The average optical density values of immunopositive signals in MTNR1a and MTNR1b during the non?breeding season were significantly higher compared with the breeding season (P < 0.01). The expression patterns of MTNR1a and MTNR1b on the HPG axis of male plateau zokor during the breeding and the non?breeding seasons revealed the potential role of melatonin in seasonal reproduction regulation.
Key words： Plateau zokor; MTNR1a gene; MTNR1b gene; Seasonal reproduction
Kang AN , Mingfang BAO , Baohui YAO , Yukun KANG , Yuchen TAN , Yanli WANG , Junhu SU . Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non‑breeding seasons[J]. ACTA THERIOLOGICA SINICA, 2022 , 42(4) : 410 -419 . DOI: 10.16829/j.slxb.150605
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