Fecal microbiota diversity analysis of the diarrheal sub-adult Qaidam horses and biomarkers screening
Received date: 2021-11-15
Accepted date: 2022-05-06
Online published: 2022-07-22
The study aims to compare the difference in diversity and composition of fecal microbiota between healthy and diarrheal sub-adult Qaidam horses. We analyzed feces of healthy (n = 13) and diarrheal (n = 3) sub-adult Qaidam horses based on 16S rRNA high-throughput sequencing, and determined the absolute abundance of related differential bacteria genera by real-time qPCR. The results indicated that Firmicutes, Bacteroidetes, Verrucomicrobia, Proteobacteria and Spirochaetes were the dominant phyla in feces from both healthy and diarrheal Qaidam horses. Compared to healthy Qaidam horses, the fecal microbial Alpha diversity was significantly lower (P < 0.05), the relative abundance of Firmicutes was relatively lower (P < 0.05), and the ralative abundance of Proteobacteria was significantly higher (P < 0.05) in diarrheal Qaidam horses. It was then deduced that the dysbiosis of these two phyla (Clostridium, Prevotella, Fibrobacter, etc.) in microecology might play an important role in Qaidam horses’ diarrhea. Additionally, 12 biomarkers (feature genera), such as Methanobrevibacter, Fibrobacter, Paludibacter, Carnobacterium and Elusimicrobium, which had significant influence on the difference of fecal bacteria composition between the two groups, were screened by a machine learning algorithm (random forest). The study compared the characteristics of fecal microbial changes in healthy and diarrheal Qaidam horses and provided a data basis for the diarrhea of livestock in the Qinghai-Tibet Plateau.
Key words： 16S rRNA sequencing; Real-time qPCR; Fecal microbiota; Biomarker; Diarrhea
Xiaoqi WANG , Wenjing HAO , Zhichao ZHANG , Jing HAN , Rujing WANG , Ziyuan DUAN . Fecal microbiota diversity analysis of the diarrheal sub-adult Qaidam horses and biomarkers screening[J]. ACTA THERIOLOGICA SINICA, 2022 , 42(4) : 432 -441 . DOI: 10.16829/j.slxb.150637
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