ORIGINAL PAPERS

Effect of temperature acclimation on thermosensitive transient receptor potential channels of liver in Brandt’s voles (Lasiopodomys brandtii)

  • LYU Jinzhen ,
  • TANG Liqiu ,
  • ZHANG Xueying ,
  • WANG Dehua
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  • 1 State Key Laboratory of Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
    2 School of Life Sciences, Shandong University, Qingdao 266237, China;
    3 CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2022-04-06

  Revised date: 2022-05-10

  Online published: 2023-01-10

Abstract

Liver is a key organ of basal metabolic thermogenesis in mammals. Thermosensitive transient receptor potential channels (Thermo-TRPs) are involved in the regulation of various physiological functions of hepatocytes. In order to understand whether Thermo-TRPs are involved in liver metabolic thermogenesis, we measured the expressions of six types of Thermo-TRPs in liver of adult Brandt's voles (Lasiopodomys brandtii) acclimated at different temperatures, and assessed the relationship between Thermo-TRPs and liver thermogenesis related proteins and signaling pathway proteins. Results showed that:(1) Compared with the hot (30℃) group, cold (4℃) acclimation increased the expression of uncoupling protein 1 (UCP1) in liver but cold acclimation decreased the expression of UCP3 in liver compared with the warm (23℃) group. (2) Six types of Thermo-TRPs were expressed in liver. Compared with the hot group, cold acclimation decreased the expression of TRP vanilloid 4 (TRPV4) and increased AMP-activated protein kinase (AMPK) in liver. (3) The level of serum thyroid hormone (T3) and the ratio of T3/T4 were significantly higher in the cold than other groups. Ratio of T3/T4 was positively correlated with UCP1 and AMPK in liver, and UCP1 was positively correlated with TRPM2 and AMPK in liver. These results suggest that liver TRPV4 and AMPK may be involved in the regulation of physiological functions such as metabolic thermogenesis under cold conditions.

Cite this article

LYU Jinzhen , TANG Liqiu , ZHANG Xueying , WANG Dehua . Effect of temperature acclimation on thermosensitive transient receptor potential channels of liver in Brandt’s voles (Lasiopodomys brandtii)[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(1) : 1 -10 . DOI: 10.16829/j.slxb.150674

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