ACTA THERIOLOGICA SINICA >

2023 , Vol. 43 >Issue 1: 21 - 32

DOI: https://doi.org/10.16829/j.slxb.150667

ORIGINAL PAPERS

Effects of exogenous leptin injection on adaptive thermogenesis in Eothenomys miletus between Kunming and Dali regions

  • CHEN Huibao ,
  • JIA Ting ,
  • ZHANG Di ,
  • ZHANG Hao ,
  • WANG Zhengkun ,
  • ZHU Wanlong
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  • 1 Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of YunnanProvince Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming 650500, China;
    2 Yunnan College of Business Management, Kunming 650106, China;
    3 Engineering Research Center of Sustainable Development and Utilization of Biomass Energy Ministry of Education, Kunming 650500, China;
    4 Key Laboratory of Yunnan Province for Biomass Energy and Environment Biotechnology, Kunming 650500, China

Received date: 2022-03-14

  Revised date: 2022-07-01

  Online published: 2023-01-10

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Abstract

Small mammals respond to environmental stress by adjusting their thermogenic capacity. To investigate the effect of exogenous leptin on the induction of adaptive thermogenesis in Eothenomys miletus from Kunming (KM) and Dali (DL), 14 voles were selected from each region and placed at 25℃ ± 1℃ with a photoperiod of 12L:12D, and daily intraperitoneal leptin injection last for 28 days. LT502 electronic balance was used to measure the body mass every two days in E. miletus, the food intake was measured by the food balance method every two days, and the resting metabolic rate (RMR) and nonshivering thermogenesis (NST) were measured by a portable respiratory metabolic measurement system every seven days. After the animals were killed on day 28, mitochondrial protein content (MtP), mitochondrial cytochrome c oxidase (COX) activity and uncoupling protein 1 (UCP1) content, serum levels of triiodothyronine (T3), thyroxine (T4), leptin, thyrotropin-releasing hormone (TRH) and adrenal corticotropin-releasing hormone (CRH) were determined by enzyme-linked immunosorbent assay (ELISA). The results showed that leptin injection significantly reduced body mass and food intake, enhanced RMR and NST, increased MtP and COX activity in liver, COX activity and UCP1 content in brown adipose tissue (BAT), and serum T3, T4, T3/T4 ratio, TRH and CRH concentrations in E. miletus from KM and DL. Leptin levels were negatively correlated with body mass and food intake, and serum T3 levels were positively correlated with NST and UCP1 levels. Moreover, body mass and food intake were higher in voles from KM region than those from DL region before injection, but RMR and NST were lower in animals from KM region. The changes in body mass in E. miletus from KM after exogenous leptin injection were higher than those from DL, while the changes in food intake, RMR, and NST were lower in KM voles than those in DL. In conclusion, exogenous injection of leptin reduced body mass and food intake and increased thermogenic capacity and energy metabolism in the two regions, suggesting that leptin was involved in their body mass and thermogenic regulation. Furthermore, E. miletus in the DL region was more sensitive to exogenous leptin injection, which may be related to low habitat temperature or poor food quality in DL.

Key words： Eothenomys miletus; Leptin; Thyroid hormones; Adaptive thermogenesis; Geographic distribution

Cite this article

CHEN Huibao , JIA Ting , ZHANG Di , ZHANG Hao , WANG Zhengkun , ZHU Wanlong . Effects of exogenous leptin injection on adaptive thermogenesis in Eothenomys miletus between Kunming and Dali regions[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(1) : 21 -32 . DOI: 10.16829/j.slxb.150667

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