Comparative analysis of gut microbial composition and functions of forest musk deer in different breeding centres
Received date: 2022-06-06
Accepted date: 2022-10-20
Online published: 2023-03-23
Intestinal diseases are common in the breeding of forest musk deer (Moschus berezovskii, FMD). Gut microbiota has evolved with the host and formed a complex microecosystem with the gastrointestinal tract of animals. To compare the effects of different breeding environments on gut microbial composition and functions of captive FMD, a total of 215 fresh feces samples were collected from 5 different breeding centres in China, and a 16S rRNA gene high-throughput sequencing was performed. The differences in gut microbial composition, diversity, and functions of FMD from different breeding centres were analyzed. The results showed that Firmicutes and Bacteroides were the absolute dominant bacteria phyla in the gut microbiota of FMD fed without compound probiotics from Qilian County, while Firmicutes and Proteobacteria were the absolute dominant bacteria in the gut microbiota of FMD fed with compound probiotics from Liangdang County and Feng County. The composition, dominant bacteria phyla, dominant bacteria genera, potential pathogenic bacteria, metabolism-related, and disease-related functions of gut microbiota in FMD from different breeding centres were significantly different. The α diversity and disease-related function enrichment of gut microbiota in FMD from Qilian were significantly lower than those from other breeding centres, and enterotype 2 was dominant, with Firmicutes, UCG-005,and Bacteroides as the marker bacteria. The relative abundance of potential pathogenic bacteria of gut microbiota in FMD from Liangdang and Feng Counties was low. We speculated that the difference in food composition might be the main factor leading to the difference in the gut microbiota of FMD from different breeding centres, and the use of compound probiotics might be an important factor leading to the decrease of α diversity and potential pathogenic bacteria. The results of this study can provide a scientific basis for the artificial breeding management of FMD, and also have certain significance for the assessment of the artificial breeding environment and the plan of reintroduction in the future.
Key words： Forest musk deer; Gut microbiota; Functions; Diversity; 16S rRNA gene sequencing
Feng JIANG , Pengfei SONG , Jingjie ZHANG , Hongmei GAO , Haijing WANG , Zhenyuan CAI , Daoxin LIU , Tongzuo ZHANG . Comparative analysis of gut microbial composition and functions of forest musk deer in different breeding centres[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(2) : 129 -140 . DOI: 10.16829/j.slxb.150701
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