Diagnosis of peste des petits ruminants and genetic evolution analysis of N and F genes of Pseudois nayaur in Qinghai
Received date: 2022-03-30
Accepted date: 2022-11-10
Online published: 2023-03-23
On January 19, 2021, an unexplained death of a Pseudois nayaur occurred in the Yikegaoli area, Balong Township, Dulan County, Haixi Prefecture, Qinghai Province. The animal was characterized by being alone, lying on the ground, physical weakness, difficulty in foraging, and black attachments around the anus. It was diagnosed as peste des petits ruminants virus (PPRV) infection by clinical symptoms, pathological autopsy, and qRT-PCR. Part of PPRV N and F genes were amplified from the diseased tissues of the dead P. nayaur by RT-PCR. Megalig, NT1, and MEGA 6.0 software were used to compare and analyze the N and F gene sequences of P. nayaur PPRV and the phylogenetic tree was constructed. The results show that the homology between the N and F genes of PPRV infecting the P. nayaur from Qinghai and the Xinjiang strain (China/Xinjiang/2015/16) was 99.43% and 99.73% respectively. Phylogenetic analysis showed that it belonged to the lineage Ⅳ. At the nucleic acid sequence level of N and F genes, it has the closest genetic relationship with the strains isolated in Xinjiang. Compared with foreign strains, the N gene is more related to the strains isolated from Senegal and Nigeria and the F gene is distantly related to foreign strains. In conclusion, Qinghai P. nayaur PPR strains belong to the gene Ⅳ lineage, which is the same pedigree as the wild virus strains currently prevalent in China.
Key words： Pseudois nayaur; Peste des petits ruminants; Diagnosis; Genetic evolution
Wei KAN , Leibin LI , Xuyang ZHAO , Lin YANG , Yu SUN , Jinshan CAI . Diagnosis of peste des petits ruminants and genetic evolution analysis of N and F genes of Pseudois nayaur in Qinghai[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(2) : 171 -181 . DOI: 10.16829/j.slxb.150673
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