To prevent Escherichia coli from causing epidemic disease in the newly established Guangyuan area of the Giant Panda National Park (GAGPNP), located in Sichuan China, an investigation was conducted to explore the epidemiological characteristics of E. coli from wild animals and their environment as well as the antibiotic resistance of wild animals in the area. A total of 124 fecal samples from wild animals, 49 water and 70 soil samples were collected from the GAGPNP from August to September 2022. 16S rRNA, the K-B method, high-throughput fluorescent quantitative PCR and PCR technology were used to isolate and identify the E. coli, perform antibiotics sensitivity test, and for the detection of antibiotics resistance genes and virulence gene, respectively. The results showed that 86 strains of E. coli were identified, and the isolation rate was 35. 39%. The E. coli isolated from the GAGPNP had a high resistance rate to amoxicillin (52. 33%) and a low resistance rate to streptomycin, gentamicin and other antibiotics (1. 16% — 9. 30%), and were sensitive to ceftazidime, chloramphenicol, florfenicol and trimethoprim. A large number of antibiotics resistance genes were carried, including multiple antibiotics resistance genes (83. 14%), sulfonamides (55. 52%), and tetracyclines (48. 52%), resistace genes with a high carrying rate, while the other antibiotics resistance genes were 26. 16% — 35. 72%. E. coli isolates carried 1 — 6 virulence genes, and the detection rate of irp2 was the highest (91. 86%), followed by ompA, ibeB, and tsh, with the detection rates of 55. 81%, 69. 77%, and 52. 33% respectively. The detection rates of FyuA, fimH, and PapA were lower (17. 44%, 19. 77% and 1. 16% respectively). None of the 86 strains of E. coli detected 5 types of diarrhoeal E. coli. This study provides an scientific data reference for the prevalence of E. coli in wild animals in GAGPNP, and provides an ecological basis for the prevention and control of zoonosis.
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