ACTA THERIOLOGICA SINICA >

2023 , Vol. 43 >Issue 4: 443 - 449

DOI: https://doi.org/10.16829/j.slxb.150758

ORIGINAL PAPERS

Effect of steroid hormones on osteoprotegerin expression in antler mesenchymal stem cells of the sika deer

  • XING Baorui ,
  • ZHAO Haiping ,
  • LI Guangyu ,
  • SUN Hongmei ,
  • MA Zefang ,
  • TAN Zhanqing ,
  • HU Xiao ,
  • LI Chenying ,
  • WU Jianhua ,
  • LIU Zhen
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  • 1 Qingdao Agricultural University, Qingdao 266109, China;
    2 Institute of Special Animal and Plant Science of Chinese Academy of Agricultural Science, Changchun 130022, China;
    3 Department of Neurosurgery, Anqiu People's Hospital, Weifang 262100, China

Received date: 2022-11-22

  Revised date: 2023-04-12

  Online published: 2023-08-02

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Abstract

The upregulation of Osteoprotegerin (OPG) expression could improve the dynamic bone homeostasis by inhibiting bone loss. During the pre-OPG expression assay, OPG showed an upward trend during osteogenesis of Antler mesenchymal stem cells (RMCs), but there was no significant difference in There was no significant difference between RMCs and control group after osteogenesis. Since peripheral blood levels of steroid hormones (Estradiol, E2; Parathyroid hormone 1-34, PTH 1-34) are closely related to antler osteogenesis, and the molecular mechanism of osteogenesis in RMCs is unknown, it is speculated that the OPG expressed by RMCs may be regulated by steroid hormones. The aim of this study was to explore the effects of steroid hormones (E2 and PTH 1-34) on OPG expression levels. The experiment explored the effects of steroid hormones on the proliferation, OPG expression and osteogenesis of RMCs using CCK-8 cell proliferation assay, qRT-PCR, Western Blot, osteogenesis induction, and alizarin red staining. The results showed that there was no significant difference in the effects of PTH 1-34 on the proliferation of RMCs during osteogenic differentiation, which could significantly promote the expression level of OPG protein to inhibit osteolysis and promote osteogenesis by upregulating the mRNA expression of osteogenic transcription factors SP7 and BGN; E2 significantly inhibited the proliferation of RMCs and could inhibit osteogenesis of RMCs by downregulating the expression of OPG and SP7. In conclusion, PTH 1-34 could upregulate the expression of OPG protein to promote osteogenesis of RMCs; E2 could downregulate the expression of OPG protein to inhibit the osteogenesis of RMCs.

Key words： Steroid hormone; Deer antler; Mesenchymal stem cells; Osteoprotegerin; Osteogenesis induction

Cite this article

XING Baorui , ZHAO Haiping , LI Guangyu , SUN Hongmei , MA Zefang , TAN Zhanqing , HU Xiao , LI Chenying , WU Jianhua , LIU Zhen . Effect of steroid hormones on osteoprotegerin expression in antler mesenchymal stem cells of the sika deer[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(4) : 443 -449 . DOI: 10.16829/j.slxb.150758

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