ACTA THERIOLOGICA SINICA >

2023 , Vol. 43 >Issue 6: 702 - 709

DOI: https://doi.org/10.16829/j.slxb.150699

ORIGINAL PAPERS

Preliminary analysis of the molecular phylogeography of Sorex unguiculatus and Sorex gracillimus

  • MEI Xiufeng ,
  • CAI He ,
  • HAN Meifeng ,
  • ZHAO Xinxu ,
  • ZHANG Chunfeng ,
  • SHI Yanjing ,
  • CHEN Huan ,
  • TIAN Xinmin ,
  • ZHANG Junsheng ,
  • LIU Zhu
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  • 1 College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157011, China;
    2 Hei Long Jiang Agricultural Economics Vocational College, Mudanjiang 157041, China

Received date: 2022-06-04

  Revised date: 2023-07-31

  Online published: 2023-11-22

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Abstract

In order to clarify the population phylogeographic relationship and subspecies differentiation of Sorex unguiculatus and Sorex gracillimus, this study analyzed the Cyt b gene sequences of S. unguiculatus (11 samples) and S. gracillimus (17 samples) collected from Northeast China together with Cyt b gene sequences of S. unguiculatus (32 samples) and S. gracillimus (22 samples) downloaded from GenBank. The different geographical populations of the two species showed different geographical patterns. In the phylogenetic tree of S. unguiculatus, there was no correlation between molecular system relation and geographical distribution. In the phylogenetic tree of S. gracillimus, samples from Northeast China were clustered into a separate clade apart from samples from Russia and Hokkaido, Japan. A similar geographical pattern was also observed in the Median-joining network analysis. Neutral tests supported S. unguiculatus has experienced population expansions in the recent past, but S. gracillimus has not experienced dramatic population expansions. The results also supported that S. unguiculatus has no subspecies differentiation. On the other hand, we found that S. gracillimus was divided into two subspecies across the geographic area of this study, one subspecies distributed in Northeast China and the other subspecies distributed in the Russian Far East (from southern Magadan to Primorsky Krai, Sakhalin Island included) and Hokkaido, Japan.

Key words： Sorex; Molecular phylogeography; Subspecies diversification

Cite this article

MEI Xiufeng , CAI He , HAN Meifeng , ZHAO Xinxu , ZHANG Chunfeng , SHI Yanjing , CHEN Huan , TIAN Xinmin , ZHANG Junsheng , LIU Zhu . Preliminary analysis of the molecular phylogeography of Sorex unguiculatus and Sorex gracillimus[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(6) : 702 -709 . DOI: 10.16829/j.slxb.150699

References

Bandelt H, Forster P, Rohl A. 1999. Median joining networks for inferring intraspecific phylogenics. Molecular Biology Evolution, 16 (1): 37-48.
Bannikova A A, Dokuchaev N E, Yudina E V, Bobretzov A V, Sheftel B I, Lebedev V S. 2010. Holarctic phylogeography of the tundra shrew (Sorex tundrensis) based on mitochondrial genes. Biological Journal of the Linnean Society, 101 (3): 721-746.
Bannikova A A, Chernetskaya D, Raspopova A, Alexandrov D, Fang Y, Dokuchaev N, Sheftel B, Lebedev V. 2018. Evolutionary history of the genus Sorex (Soricidae, Eulipotyphla) as inferred from multigene data. Zoologica Scripta, 47 (5): 518-538.
Burgin C J, He K. 2018. Family Soricidae (shrews). In: Wilson D E, Mittermeier R A eds. Handbook of the Mammals of the World. Vol. 8. Insectivores, Sloths and Colugos. Barcelona, Spain:Lynx Editions.
Hoffmann R S. 1987. A review of the systematics and distribution of Chinese red-toothed shrews (Mammalia: Soricinae). Acta Theriologica Sinica, 7 (2): 100-139.
Hope A G, Waltari E, Dokuchaev N E, Abramov S, Dupal T, Tsvetkova A, Henttonen H, Macdowald S O, Cook J A. 2010. High-latitude diversification within Eurasian least shrews and Alaska tiny shrews (Soricidae). Journal of Mammalogy, 91 (5): 1041-1057.
Hutchison D W, Templeton A R. 1999. Correlation of pairwise genetic and geographic distance measures: inferring the relative influences of gene flow and drift on the distribution of genetic variability. Evolution, 53 (6): 1898-1914.
Hutterer R. 2005. Order Soricomorpha. In: Wilson D E, Reeder D A eds. Mammal Species of the World: A Taxonomic and Geographic Reference. 3rd ed. Baltimore: Johns Hopkins University Press.
Irwin D M, Kocher T D, Wilson A C. 1991. Evolution of the Cytochrome b gene of mammals. Molecular Evolution, 32 (2):128-144.
Jiang Z Y, Peng Y L, Hu X X, Zhou Y F, Liu J Q. 2011. Cytoplasmic DNA variation in and genetic delimitation of Abies nephrolepis and Abies holophylla in northeastern China. Canadian Journal of Forest Research, 41 (7): 1555-1561.
Li B, Malyarchuk B, Ma Z P, Derenko M, Zhao J P, Zhou X L. 2013.Phylogeography of sable (Martes zibellina L. 1758) in the southeast portion of its range based on mitochondrial DNA variation:highlighting the evolutionary history of the sable. Acta Theriologica, 58 (2): 139-148.
Liu Z, Li B, Ma J Z, Zheng D, Xu Y C. 2014. Phylogeography and genetic diversity of the red squirrel (Sciurus vulgaris) in China: Implications for the species’postglacial expansion history. Mammalian Biology, 79 (4): 247-253.
Liu Z, Xie R X, Liu H, Jin Z M. 2016. The slender shrew (Sorex gracillimus Thomas, 1907; Insetivora: Soricidae) was found in Hengdaohezi of Heilongjiang Province, China. Chinese Journal of Zoology, 51 (3): 487-491. (in Chinese)
Liu Z, Zhang J S, Bai W, Liu H, Xie R X, Yang X, Jin Z M. 2019. Classification and distribution of Soricids in Northeastern China. Acta Theriologica Sinica, 39 (1): 24-42. (in Chinese)
Liu Z, Jiang W J, Wang A N, Yang X, Jin Z M, Tian X M, Li D W. 2020a. Genetic diversity and molecular phylogeography of Sorex caecutiens in northeast of China. Acta Theriologica Sinica, 40(3): 249-260. (in Chinese)
Liu Z, Wang Q Q, Bai W, Li B Q, Tian X M, Li D W, Zhang J S. 2020b. Preliminary study on genetic diversity and genetic structure of Sorex isodon in Northeast of China. Chinese Journal of Applied Ecology, 31 (2): 634-642. (in Chinese)
Librado P, Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphismdata. Bioinformatics, 25 (11):1451-1452.
Ohdachi S, Masuda R, Abe H, Adachi J, Dokuchaev N E, Haukisalmi V, Yoshida M C. 1997a. Phylogeny of Eurasian soricine shrews(Insectivora, Mammalia) inferred from the mitochondrial cytochrome b gene sequences. Zoological Science, 14 (3): 527-532.
Ohdachi S, Masuda R, Abe H, Dokuchaev N E. 1997b. Biogeographical history of northeastern Asiatic soricine shrews (Insectivora, Mammalia). Researches on Population Ecology, 39 (2):157-162.
Ohdachi S, Dokuchaev N E, Hasegawa M, Masuda R. 2001. Intraspecific phylogeny and geographical variation of six species of northeastern Asiatic Sorex shrews based on the mitochondrial cytochrome b sequences. Molecular Ecology, 10 (9): 2199-2213.
Raspopova A A, Bannikova A A, Lebedev V S. 2018. The phylogeography and demographic history of the common shrew Sorex araneus L., 1758 (Eulipotyphla, Mammalia). Russian Journal of Genetics, 54 (12): 1452-1465.
Ren G P, Abbott R J, Zhou Y F, Zhang L R, Peng Y L, Liu J Q. 2012. Genetic divergence, range expansion and possible homoploid hybrid speciation among pine species in Northeast China. Heredity, 108 (5): 552-562.
Rozas J, Sánchez-DelBarrio J C, Messeguer X, Rozas R. 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19 (18): 2496-2497.
Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9):1312-1313.
Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25 (24): 4876-4882.
Vila C, Amorim I R, Leonard J A, Posada D, Castroviejo J, Petrucci-Fonseca F, Crandall K A, Ellegren H, Wayne R K. 1999. Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus. Molecular Ecology, 8 (12):2089-2103.
Walker C W, Vila C, Landa A, Linden M, Ellegren H. 2001. Genetic variation and population structure in Scandinavian wolverine(Gulo gulo) populations. Molecular Ecology, 10 (1): 53-63.
Zeng Y F, Wang W T, Liao W J, Wang H F, Zhang D Y. 2015. Multiple glacial refugia for cool-temperate deciduous trees in northern East Asia: The Mongolian oak as a case study. Molecular Ecology, 24 (22): 5676-5691.
刘铸, 解瑞雪, 刘欢, 金志民. 2016. 黑龙江省横道河子地区发现细鼩鼱 (食虫目: 鼩鼱科). 动物学杂志, 51 (3): 487-491.
刘铸, 张隽晟, 白薇, 刘欢, 解瑞雪, 杨茜, 金志民. 2019. 中国东北地区鼩鼱科动物分类与分布. 兽类学报, 39 (1): 24-42.
刘铸, 蒋纹静, 王奥男, 杨茜, 金志民, 田新民, 李殿伟. 2020a. 东北地区中鼩鼱遗传多样性与分子系统地理学. 兽类学报, 40 (3):249-260.
刘铸, 汪青青, 白薇, 李博琦, 田新民, 李殿伟, 张隽晟. 2020b. 中国东北地区远东鼩鼱遗传多样性与遗传结构. 应用生态学报, 31(2): 634-642.
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