The expression profile of miRNA in the different tissues of forest musk deer (Moschus berezovskii)

YU Miaojie; XU Zhongxian; JIANG Xuemei; WANG Chunhua; ZHAO Chanjuan; QI Wenhua; JIE Hang

doi:10.16829/j.slxb.150744

ACTA THERIOLOGICA SINICA >

2023 , Vol. 43 >Issue 6: 723 - 733

DOI: https://doi.org/10.16829/j.slxb.150744

ORIGINAL PAPERS

The expression profile of miRNA in the different tissues of forest musk deer (Moschus berezovskii)

YU Miaojie ,
XU Zhongxian ,
JIANG Xuemei ,
WANG Chunhua ,
ZHAO Chanjuan ,
QI Wenhua ,
JIE Hang

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1 College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China;
2 College of Life Sciences, China West Normal University, Nanchong 637009, China;
3 College of Animal Medicine, Hunan Agricultural University, Changsha 410125, China;
4 Chongqing Three Gorges Vocational College, Chongqing 404155, China;
5 Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China

Received date: 2022-10-25

Revised date: 2023-07-29

Online published: 2023-11-22

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Abstract

Forest musk deer (Moschus berezovskii) is a medicinal economic species and the musk secreted by the male musk deer is an important component of Chinese patent medicine and perfume, which has high medicinal and economic value. miRNAs play an important role in animal cell development, differentiation, apoptosis, and disease development, which are selectively expressed in different tissues. The heart, liver, spleen, lung, kidney, and muscle tissues of forest musk deer were high-throughput sequenced by using RNA-seq technology on the Hi-seq 2500 sequencing platform. The microRNA expression of these 6 tissues was analyzed by using biostatistics and bioinformatics methods and RT-qPCR was used to verify the sequencing results. The results showed that 1 650 miRNAs co-expressed in the heart, liver, spleen, lung, kidney, and muscle tissues of forest musk deer, of which there were 86, 54, 44, 68, 83, and 50 specific miRNAs, respectively (P < 0.01). The expression levels of co-expressed miRNAs were significantly different in these six types of tissues (P < 0.05) and the expression levels of some specific miRNAs in the heart were much higher than that in other tissues. KEGG analysis showed that the target genes of specific miRNAs in the liver of forest musk deer were closely related to the Wnt signaling pathway (ko04310), cAMP signaling pathway (ko04024), ECM-receptor interaction (ko04512), Notch signaling pathway (ko04330), and AMPK signaling pathway (ko04152) (P < 0.01). Nine miRNAs were randomly selected for verification with RT-qPCR in liver tissue of health and abscess forest musk deer and the results were consistent with the sequencing results.

Key words： MicroRNA; Expression profile; Expression analysis; Fluorescence quantitative PCR; Forest musk deer

Cite this article

YU Miaojie , XU Zhongxian , JIANG Xuemei , WANG Chunhua , ZHAO Chanjuan , QI Wenhua , JIE Hang . The expression profile of miRNA in the different tissues of forest musk deer (Moschus berezovskii)[J]. ACTA THERIOLOGICA SINICA, 2023 , 43(6) : 723 -733 . DOI: 10.16829/j.slxb.150744

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