Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain, Ningxia, China

YIN Cai; LI Zhixin; LI Lin; DI Jing; ZHOU Haining; ZHANG Yuling; BAI Taotao; WANG Yu-mei; ZHAO Yuan; LIN Qiuxiong; WU Yawen; MA Long; LI Lijuan; WANG Xiaoliang; ZENG Qiaoying

doi:10.16829/j.slxb.150864

ACTA THERIOLOGICA SINICA >

2024 , Vol. 44 >Issue 4: 497 - 505

DOI: https://doi.org/10.16829/j.slxb.150864

ORIGINAL PAPERS

Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain, Ningxia, China

YIN Cai ,
LI Zhixin ,
LI Lin ,
DI Jing ,
ZHOU Haining ,
ZHANG Yuling ,
BAI Taotao ,
WANG Yu-mei ,
ZHAO Yuan ,
LIN Qiuxiong ,
WU Yawen ,
MA Long ,
LI Lijuan ,
WANG Xiaoliang ,
ZENG Qiaoying

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1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2 Ningxia Hui Autonomous Center for Animal Disease Control and Prevention, Yinchuan 750011, China;
3 School of Agriculture, Ningxia University, Yinchuan 750021, China;
4 China Animal Health and Epidemiology Center, Qingdao 266033, China;
5 Helan County Center for Animal Disease Control and Prevention, Helan 750200, China

Received date: 2023-10-08

Revised date: 2024-01-29

Online published: 2024-08-03

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Abstract

From 2018 to 2023, wild Pseudois nayaur were found to have died of unknown causes continuously in Helan Mountain National Nature Reserve of Ningxia, which was confirmed to be positive for peste des petits ruminants virus (PPRV) nucleic acid through anatomical sampling and real-time fluorescent RT-PCR detection. Vero cells were used to isolate some strains of PPRV, and RT-PCR was used to amplify the full-length fragments of PPRV F gene from diseased domestic sheep and wild animal samples in Ningxia. DNASTAR Lasergene and MEGA X were used to compare and analyze the PPRV F gene sequence and construct a phylogenetic tree to analyze its molecular genetic characteristics. The results show: the homology of F gene of PPRV strains infected by domestic sheep and Pseudois nayaur of Helan Mountain in different years in Ningxia was above 99%, among which the 2023 strain had the highest homology of 99. 8% with that in January 2018 and 2019, and 99. 6% with the PPR epidemic strains in 2014 and 2016 in Ningxia and China/XJYL/2013(KM091959. 1) of Xinjiang. Genetic analysis showed that the PPRV of wild Pseudois nayaur and domestic sheep in Ningxia belonged to the central Asian branch of gene Ⅳ line. Therefore, it is speculated that the epidemic of PPR in Ningxia and neighboring provinces may have spread in the wild animals of Helan Mountain through different ways, and it is necessary to strengthen the surveillance of PPR and other wild animal diseases.

Key words： Helan Mountain in Ningxia; Pseudois nayaur; Peste des petits ruminants; Molecular genetic characteristics

Cite this article

YIN Cai , LI Zhixin , LI Lin , DI Jing , ZHOU Haining , ZHANG Yuling , BAI Taotao , WANG Yu-mei , ZHAO Yuan , LIN Qiuxiong , WU Yawen , MA Long , LI Lijuan , WANG Xiaoliang , ZENG Qiaoying . Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain, Ningxia, China[J]. ACTA THERIOLOGICA SINICA, 2024 , 44(4) : 497 -505 . DOI: 10.16829/j.slxb.150864

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