Construction of the expression vector for the PRDX1 gene in Tarim red deer and its bioinformatic analysis
Received date: 2024-11-06
Accepted date: 2025-04-18
Online published: 2025-04-18
To investigate the structural and functional characteristics of Peroxiredoxin 1 (PRDX1)，which may play an antioxidant role in the adaptation of the Tarim red deer (Cervus elaphus yarkandensis) to arid environments, this study amplified the coding sequence (CDS) of the PRDX1 gene (CeyPRDX1) using reverse transcription PCR (RT-PCR). The CDS sequence was successfully cloned into the pcDNA3.1 (+) vector to construct an expression vector and named pcDNA3.1 (+) -CeyPRDX1. The pLJM1-EGFP Lentivirus vector with EGFP fluorescent label was used to construct overexpression vector of CeyPRDX1 (pLJM1-CeyPRDX1-EGFP), and its subcellular localization was observed in the cytoplasm and nucleus of human HaCaT cells. The results of homology comparison and phylogenetic tree analysis indicated that CeyPRDX1 has the highest homology and the closest phylogenetic relationship with Eastern European red deer (Cervus elaphus) and red deer (Cervus canadensis). Bioinformatic analysis revealed that CeyPRDX1 is a conserved, hydrophilic protein without N-glycosylation or transmembrane regions, and interacts with various antioxidant proteins. This study provides a foundation for future functional research on the CeyPRDX1 gene.
Key words： Tarim red deer; PRDX1; Vector construction; Bioinformatic analysis
Buweihailiqiemu ABABAIKERI , Aibibula ABUDULA , Buhailiqiemu YIMINGAILI , Huabin ZHAO . Construction of the expression vector for the PRDX1 gene in Tarim red deer and its bioinformatic analysis[J]. ACTA THERIOLOGICA SINICA, 2026 , 46(2) : 153 -170 . DOI: 10.16829/j.slxb.151026
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