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野生四川短尾鼩尿液中挥发性化学成分的分析

宗浩 胡涛 杜蓉 李佳玲 冯丹 汪茜 雷子勇 李琪 付长坤   

  1. (四川师范大学生命科学学院,成都610101)
  • 出版日期:2019-11-30 发布日期:2019-11-27
  • 通讯作者: 付长坤 E-mail:fckgoodxs@163.com
  • 基金资助:
    国家自然科学基金(31672304);四川师范大学科研创新团队基金

Analysis of urinary volatile constituents of wild Anourosorex squamipes

ZONG Hao,HU Tao,DU Rong,LI Jialing,FENG Dan,WANG Qian,LEI Ziyong,LI Qi, FU Changkun#br#   

  1. (College of Life Sciences,Sichuan Normal University,Chengdu 610101,China)
  • Online:2019-11-30 Published:2019-11-27

摘要: 尿液在哺乳动物间的化学通讯中具有重要作用。本研究采用顶空固相微萃取-气质联用(HS-SPME-GC/MS)方法分析四川短尾鼩尿液中挥发性物质化学组成。结果表明,(1)通过检索国家标准与技术研究所质谱库,发现四川短尾鼩尿液中的化学成分主要为醛类、醇类、酮类、杂环、酯类、烷烃类、芳香烃类、含硫化合物、烯烃类、酚类等86种化合物,并且不同个体气味包含特有的挥发性化合物;(2)雌雄个体尿液中分别特有的挥发性化合物共计10种,其中雌性特有6种,雄性特有4种,表明四川短尾鼩尿液的化学成分存在性别差异。雌性的尿液中挥发性化合物76种,雄性只有33种,说明四川短尾鼩尿液中化学信号雌性多于雄性;(3)四川短尾鼩尿液中不同化学成分相对含量不同,同一组分在不同个体间相对含量存在差异。本研究有助于揭示四川短尾鼩尿液挥发性成分的化学通讯功能,并为引诱剂的研制提供基础资料。

关键词: 化学通讯, 化学成分, 四川短尾鼩, 尿液

Abstract: Urine plays an important role in chemical communications among mammals. In this study,headspace-soild phase microextraction(HS-SPME) and gas chromatography-mass spectrometry(GC-MS) were used to analyze the urine compositions in Anourosorex squamipes. Our reserach showed that 1) Through searching the mass spectrometry library of the National Institute of Standards and Technology(NIST),we found that the 86 chemical compounds of their urine mainly included toaldehydes,alcohols,ketones,heterocycles,esters,hydrocarbons,aromatic hydrocarbons,sulfurcompounds,olefins,phenols,etc,and different individual odors contained unique volatile compounds. 2) Ten volatile compounds in urine were specific to male and female individuals. Six volatile compounds in urine were found only in females while four compounds were found only in males.This indicates that there are significant sexual differences in the chemical compositions of urine.There are 76 volatile substances in the urine of the female while only 33 in the urine of the male,also indicating that females had more chemical signals in urine than males. 3)The relative contents of different chemical components in urine were different,and the relative contents of the same component among different individuals were different. By analyzing the chemical composition of the urine of Anourosorex squamipes,This study will further our understanding of the chemical communication function of volatile components in the urine of Anourosorex squamipes,and to provide basic information for the development of attractants.

Key words: Chemical communication, Volatile constituents, Anourosorex squamipes, Urine