外源瘦素注射对昆明和大理地区大绒鼠适应性产热的影响

doi:10.16829/j.slxb.150667

兽类学报 ›› 2023, Vol. 43 ›› Issue (1): 21-32.DOI: 10.16829/j.slxb.150667

外源瘦素注射对昆明和大理地区大绒鼠适应性产热的影响

陈辉宝¹, 贾婷², 章迪², 张浩¹, 王政昆¹, 朱万龙^1,3,4

1 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室, 云南师范大学生命科学学院, 昆明 650500;
2 云南经济管理学院, 昆明 650106;
3 生物能源持续开发利用教育部工程研究中心, 昆明 650500;
4 云南省生物质能与环境生物技术重点实验室, 昆明 650500

收稿日期:2022-03-14 修回日期:2022-07-01 发布日期:2023-01-10
通讯作者: 朱万龙,E-mail:zwl_8307@163.com
作者简介:陈辉宝(1995-),男,硕士研究生,主要从事动物生理生态学研究.E-mail:chb_9511@163.com
基金资助:
国家自然科学基金(32160254)；云南省中青年学术和技术带头人后备人才项目(2019HB013)；云南省万人计划青年拔尖人才项目(YNWR-QNRC-2019-047)

Effects of exogenous leptin injection on adaptive thermogenesis in Eothenomys miletus between Kunming and Dali regions

CHEN Huibao¹, JIA Ting², ZHANG Di², ZHANG Hao¹, WANG Zhengkun¹, ZHU Wanlong^1,3,4

1 Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of YunnanProvince Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming 650500, China;
2 Yunnan College of Business Management, Kunming 650106, China;
3 Engineering Research Center of Sustainable Development and Utilization of Biomass Energy Ministry of Education, Kunming 650500, China;
4 Key Laboratory of Yunnan Province for Biomass Energy and Environment Biotechnology, Kunming 650500, China

Received:2022-03-14 Revised:2022-07-01 Published:2023-01-10

摘要/Abstract

摘要： 小型哺乳动物通过产热能力的调整来应对环境的胁迫。为探究外源瘦素对不同地区大绒鼠(Eothenomys mi-letus)适应性产热的影响，选取云南昆明和大理地区捕获的大绒鼠各14只，置于25℃±1℃，光周期为 12L∶12D的环境中，每日腹腔注射瘦素，持续28d。以LT502电子天平每两天测定大绒鼠的体重，采用食物平衡法每两天测定大绒鼠摄食量，以便携式呼吸代谢测量系统每7天测定静止代谢率(RMR)、非颤抖性产热(NST)。第28天处死动物后，采用酶联免疫吸附法测定线粒体蛋白含量、线粒体细胞色素c氧化酶(COX)活性、解偶联蛋白1(UCP1)含量、血清三碘甲状腺原氨酸(T₃)、甲状腺素(T₄)、瘦素水平以及促甲状腺激素释放激素(TRH)和促肾上腺皮质激素释放激素(CRH)水平。结果表明，注射瘦素后昆明和大理地区大绒鼠的体重和摄食量显著降低，RMR和NST增强，肝脏中线粒体蛋白含量和COX活性，褐色脂肪组织(BAT)中COX活性和UCP1含量，及血清T₃、T₄、T₃/T₄比值、TRH和CRH浓度均增加。瘦素水平与体重、摄食量呈负相关，血清T₃水平与NST和UCP1含量呈正相关。此外，注射前昆明地区大绒鼠的体重和摄食量高于大理地区，但RMR和NST低于大理地区。外源注射瘦素后昆明地区大绒鼠的体重变化高于大理地区大绒鼠，而摄食量、RMR和NST的变化低于大理地区大绒鼠。总之，外源瘦素注射降低了两地区大绒鼠的体重和摄食量，增加了其产热能力和能量代谢，说明瘦素参与了其体重和产热调节。此外，大理地区大绒鼠对外源瘦素注射更为敏感，这可能与该地区大绒鼠栖息环境温度较低，食物质量较差有关。

关键词: 大绒鼠, 瘦素, 甲状腺激素, 适应性产热, 地理分布

Abstract: Small mammals respond to environmental stress by adjusting their thermogenic capacity. To investigate the effect of exogenous leptin on the induction of adaptive thermogenesis in Eothenomys miletus from Kunming (KM) and Dali (DL), 14 voles were selected from each region and placed at 25℃ ± 1℃ with a photoperiod of 12L:12D, and daily intraperitoneal leptin injection last for 28 days. LT502 electronic balance was used to measure the body mass every two days in E. miletus, the food intake was measured by the food balance method every two days, and the resting metabolic rate (RMR) and nonshivering thermogenesis (NST) were measured by a portable respiratory metabolic measurement system every seven days. After the animals were killed on day 28, mitochondrial protein content (MtP), mitochondrial cytochrome c oxidase (COX) activity and uncoupling protein 1 (UCP1) content, serum levels of triiodothyronine (T₃), thyroxine (T₄), leptin, thyrotropin-releasing hormone (TRH) and adrenal corticotropin-releasing hormone (CRH) were determined by enzyme-linked immunosorbent assay (ELISA). The results showed that leptin injection significantly reduced body mass and food intake, enhanced RMR and NST, increased MtP and COX activity in liver, COX activity and UCP1 content in brown adipose tissue (BAT), and serum T₃, T₄, T₃/T₄ ratio, TRH and CRH concentrations in E. miletus from KM and DL. Leptin levels were negatively correlated with body mass and food intake, and serum T₃ levels were positively correlated with NST and UCP1 levels. Moreover, body mass and food intake were higher in voles from KM region than those from DL region before injection, but RMR and NST were lower in animals from KM region. The changes in body mass in E. miletus from KM after exogenous leptin injection were higher than those from DL, while the changes in food intake, RMR, and NST were lower in KM voles than those in DL. In conclusion, exogenous injection of leptin reduced body mass and food intake and increased thermogenic capacity and energy metabolism in the two regions, suggesting that leptin was involved in their body mass and thermogenic regulation. Furthermore, E. miletus in the DL region was more sensitive to exogenous leptin injection, which may be related to low habitat temperature or poor food quality in DL.

Key words: Eothenomys miletus, Leptin, Thyroid hormones, Adaptive thermogenesis, Geographic distribution

中图分类号:

Q494

陈辉宝, 贾婷, 章迪, 张浩, 王政昆, 朱万龙. 外源瘦素注射对昆明和大理地区大绒鼠适应性产热的影响[J]. 兽类学报, 2023, 43(1): 21-32.

CHEN Huibao, JIA Ting, ZHANG Di, ZHANG Hao, WANG Zhengkun, ZHU Wanlong. Effects of exogenous leptin injection on adaptive thermogenesis in Eothenomys miletus between Kunming and Dali regions[J]. ACTA THERIOLOGICA SINICA, 2023, 43(1): 21-32.

参考文献

Abelenda M, Ledesma A, Rial E, Puerta M. 2003. Leptin administra-tion to cold-acclimated rats reduces both food intake and brown adipose tissue thermogenesis.Journal of Thermal Biology,28(6-7):525-530.
Ahima R S. 2005. Central actions of adipocyte hormones. Trends in Endocrinology & Metabolism Tem, 16 (7):307-313.
Baskin D G, Figlewicz Lattemann D, Seeley R J, Woods S C, Porte D Jr, Schwartz M W. 1999. Insulin and leptin:dual adiposity sig-nals to the brain for the regulation of food intake and body weight. Brain Reseach, 848 (1-2):114-123.
Bowen H, Mitchell T D, Harris R B. 2003. Method of leptin dosing, strain, and group housing influence leptin sensitivity in high-fatfed weanling mice. American Journal of Physiology Regulatory Integrative & Comparative Physiology, 284 (1):R87-R100.
Buonfiglio D, Parthimos R, Dantas R, Cerqueira Silva R, Gomes G, Andrade-Silva J, Ramos-Lobo A, Amaral F G, Matos R, Sinésio J Jr, Motta-Teixeira L C, Donato J Jr, Reiter R J, Cipolla-Neto J. 2018. Melatonin absence leads to long-term leptin resistance and overweight in rats. Frontiers in Endocrinology, 9:122.
Butiaeva L I, Slutzki T, Swick H E, Bourguignon C, Robins S C, Liu X, Storch K F, Kokoeva M V. 2021. Leptin receptor-expressing pericytes mediate access of hypothalamic feeding centers to circu-lating leptin. Cell Metab, 33 (7):1433-1448. e5.
Campfield L A, Smith F J, Guisez Y, Devos R, Burn P. 1995. Recom-binant mouse OB protein:evidence for a peripheral signal linking adiposity and central neural networks. Science, 269 (5223):546-549.
Cancello R, Zingaretti M C, Sarzani R, Ricquier D, Cinti S. 1998. Leptin and UCP1 genes are reciprocally regulated in brown adi-pose tissue. Endocrinology, 139 (11):4747-4750.
Commins S P, Watson P M, Frampton I C, Gettys T W. 2001. Leptin selectively reduces white adipose tissue in mice via a UCP1-de-pendent mechanism in brown adipose tissue. American Journal of Physiology-Endocrinology & Metabolism, 280 (2):E372-E377.
Cannon B, Nedergaard J. 2004. Brown adipose tissue:function and physiological significance. Physiological Reviews, 84 (1):277-359.
Demas G E, Bowers R R, Bartness T J, Gettys T W. 2002. Photoperi-odic regulation of gene expression in brown and white adipose tis-sue of Siberian hamsters (Phodopus sungorus). AJP Regulatory Integrative and Comparative Physiology, 282 (1):R114-R121.
Friedman J M, Halaas J L. 1998. Leptin and the regulation of body weight in mammals. Nature, 395 (6704):793-770.
Fischer A W, Cannon B, Nedergaard J. 2020. Leptin:Is it thermo-genic? Endocrine Reviews, 41 (2):232-260.
Gullicksen P S, Flatt W P, Dean R G, Hartzell D L, Baile C A. 2002. Energy metabolism and expression of uncoupling proteins 1, 2, and 3 after 21 days of recovery from intracerebroventricular mouse leptin in rats. Physiology & Behavior, 75 (4):473-482.
Gutman R, Choshniak I, Kronfeld-Schor N. 2006. Defending body mass during food restriction in Acomys russatus:a desert rodent that does not store food. American Journal of Physiology Regula-tory Integrative & Comparative Physiology, 290 (4):R881-R891.
Halaas J L, Gajiwala K S, Maffei M, Cohen S L, Chait B T, Rabinow-itz D, Lallone R L, Burley S K, Friedman J M. 1995. Weightreducing effects of the plasma protein encoded by the obese gene. Science, 269 (5223):543-546.
Halaas J L, Boozer C, Blair-West J, Fidahusein N, Denton D A, Fried-man J M. 1997. Physiological response to long-term peripheral and central leptin infusion in lean and obese mice. Proceedings of the National Academy of Sciences of the United States of America, 94 (16):8878-8883.
Haynes W G, Morgan D A, Walsh S A, Mark A L, Sivitz W I. 1997. Receptor-mediated regional sympathetic nerve activation by leptin. The Journal of Clinical Investigation, 100 (2):270-278.
Heldmaier G, Steinlechner S, Rafael J. 1982. Nonshivering thermo-genesis and cold resistance during seasonal acclimatization in the Djungarian hamster. Journal of Comparative Physiology B, 149(1):1-9.
Hermann G E, Barnes M J, Rogers R C. 2006. Leptin and thyrotropin-releasing hormone:cooperative action in the hind-brain to activate brown adipose thermogenesis. Brain Reseach, 1117 (1):118-124.
Hwa J J, Fawzi A B, Graziano M P, Ghibaudi L, Williams P, Van Heek M, Davis H, Rudinski M, Sybertz E, Strader C D. 1997. Leptin increases energy expenditure and selectively promotes fat metabo-lism in ob/ob mice. American Journal of Physiology, 272 (4 Pt 2):R1204-R1209.
Johnson M S, Onorato D P, Gower B A, Nagy T R. 2004. Weight change affects serum leptin and corticosterone in the collared lem-ming. General & Comparative Endocrinology, 136 (1):30-36.
Klingenspor M, Niggemann H, Heldmaier G. 2000. Modulation of leptin sensitivity by short photoperiod acclimation in the Djungarian hamster, Phodopus sungorus. Journal of Comparative Physi-ology B:Biochemical, Systemic, and Environmental Physiology, 170 (1):37-43.
Kim M S, Namkoong C, Kim H S, Jang P G, Kim Pak Y M, Katakami H, Park J Y, Lee K U. 2004. Chronic central administration of ghrelin reverses the effects of leptin. International Journal Obe-sity Relat Metab Disord, 28 (10):1264-1271.
Król E, Duncan J S, Redman P, Morgan P J, Mercer J G, Speakman J R. 2006. Photoperiod regulates leptin sensitivity in field voles, Microtus agrestis. Journal of Comparative Physiology B, 176 (2):153-163.
Król E, Speakman J R. 2007. Regulation of body mass and adiposity in the field vole, Microtus agrestis:a model of leptin resistance. Journal of Endocrinology, 192 (2):271-278.
Levin N, Nelson C, Gurney A, Vandlen R, de Sauvage F. 1996. De-creased food intake does not completely account for adiposity re-duction after ob protein infusion. Proceedings of the National Academy of Sciences, 93 (4):1726-1730.
Lisboa P C, Oliveira K J, Cabanelas A, Ortiga-Carvalho T M, Pazos-Moura C C. 2003. Acute cold exposure, leptin, and soma-tostatin analog (octreotide) modulate thyroid 5'-deiodinase activ-ity. American Journal of Physiology-Endocrinology & Metabo-lism, 284 (6):E1172-E1176.
Li X S, Wang D H. 2005. Regulation of body weight and thermogen-esis in seasonally acclimatized Brandt's voles (Microtus brandti). Hormones and Behavior, 48 (3):321-328.
Luo Z X, Chen W, Gao W. 2000. Fauna of China. Beijing:Science Press, 89-95. (in Chinese)
Mullur R, Liu Y Y, Brent G A. 2014. Thyroid hormone regulation of metabolism. Physiological Reviews, 94 (2):355-382.
Pelleymounter M A, Cullen M J, Baker M B, Hecht R, Winters D, Boone T, Collins F. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science, 269 (5223):540-543.
Ren Y, Liu P F, Zhu W L, Zhang H, Cai J H. 2020. Higher altitude and lower temperature regulate the body mass and energy metabo-lism in male Eothenomys miletus. Pakistan Journal of Zoology, 52 (1):139-146.
Rousseau K, Atcha Z, Cagampang F R, Le Rouzic P, Stirland J A, Iva-nov T R, Ebling F J, Klingenspor M, Loudon A S. 2002. Photo-periodic regulation of leptin resistance in the seasonally breeding Siberian hamster (Phodopus sungorus). Endocrinology, 143 (8):3083-3095.
Rousseau K, Atcha Z, Loudon A S. 2003. Leptin and seasonal mam-mals. Journal of Neuroendocrinology, 15 (4):409-414.
Scarpace P J, Matheny M, Pollock B H, Tümer N. 1997. Leptin in-creases uncoupling protein expression and energy expenditure. American Journal of Physiology, 273 (1Pt1):E226-E230.
Scarpace P J, Matheny M. 1998. Leptin induction of UCP1 gene ex-pression is dependent on sympathetic innervation. American Jour-nal of Physiology, 275 (2):E259-E264.
Schwartz M W, Woods S C, Porte D Jr, Seeley R J, Baskin D G. 2000. Central nervous system control of food intake. Nature, 404(6778):661-671.
Shek E W, Scarpace P J. 2000. Resistance to the anorexic and thermo-genic effects of centrally administrated leptin in obese aged rats. Regulatory Peptides, 92 (1-3):65-71.
Silva J E. 2003. The thermogenic effect of thyroid hormone and its clinical implications. Annals of Internal Medicine, 139 (3):205-213.
Tang G B, Cui J G, Wang D H. 2009. Role of hypoleptinemia during cold adaptation in Brandt's voles (Lasiopodomys brandtii). American Journal of Physiology Regulatory Integrative & Com-parative Physiology, 297 (5):R1293-R1301.
Tups A, Helwig M, Khorooshi R M, Archer Z A, Klingenspor M, Mer-cer J G. 2004. Circulating ghrelin levels and central ghrelin re-ceptor expression are elevated in response to food deprivation in a seasonal mammal (Phodopus sungorus). Journal of Neuroendo-crinology, 16 (11):922-928.
Tups A. 2009. Physiological models of leptin resistance. Journal of Neuroendocrinology, 21 (11):961-971.
Ukropec J, Anunciado R V, Ravussin Y, Kozak L P. 2006. Leptin is re-quired for uncoupling protein-1-independent thermogenesis dur-ing cold stress. Endocrinology, 147 (5):2468-2480.
Van Heek M, Compton D S, France C F, Tedesco R P, Fawzi A B, Gra-ziano M P, Sybertz E J, Strader C D, Davis H R Jr. 1997. Dietinduced obese mice develop peripheral, but not central, resistance to leptin. Journal of Clinical Investigation, 99 (3):385-390.
Wang Z K, Liu L, Liang Z Q, Li Q F, Sun R Y. 1999. Thermogenic characteristics and body temperature regulation in the oriental voles (Eothenomys miletus). Acta Theriologica Sinica, 19 (4):276-286. (in Chinese)
Wang H, Yang X M, Liu C Y, Wang Z K. 2006. Thermoregulatory and thermogenic properties in Eothenomys miletus and Apodemus chevrieri. Acta Theriologica Sinica, 26 (2):144-151. (in Chi-nese)
Wang J M, Zhang Y M, Wang D H. 2006a. Seasonal regulations of en-ergetics, serum concentrations of leptin, and uncoupling protein 1 content of brown adipose tissue in root voles (Microtus oecono-mus) from the Qinghai-Tibetan Plateau. Journal of Comparative Physiology B, 176 (7):663-671.
Wang J M, Zhang Y M, Wang D H. 2006b. Seasonal thermogenesis and body mass regulation in plateau pikas (Ochotona curzoniae). Oecologia, 149 (3):373-382.
Weiner J, Roth L, Kranz M, Brust P, Boelen A, Klöting N, Heiker J T, Blüher M, Tönjes A, Pfluger P T, Stumvoll M, Mittag J, Krause K. 2021. Leptin counteracts hypothermia in hypothyroidism through its pyrexic effects and by stabilizing serum thyroid hor-mone levels. Molecular Metabolism, 54:101348.
Xu J, Bartolome C L, Low C S, Yi X, Chien C H, Wang P, Kong D. 2018. Genetic identification of leptin neural circuits in energy and glucose homeostases. Nature, 556 (7702):505-509.
Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman J M. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature, 372 (6505):425-432.
Zhang J V, Ren P G, Avsian-Kretchmer O, Luo C W, Rauch R, Klein C, Hsueh A J. 2005. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science, 310(5750):996-999.
Zhang Z Q, Liu Q S, Li J Y, Wang D H. 2006. Seasonal changes in thermogenic properties of brown adipose tissue and liver in Mon-golian gerbils Meriones unguiculatus. Acta Zoologica Sinica, 52(6):1034-1041. (in Chinese)
Zhang Z Q, Wang D H. 2007. Seasonal changes in thermogenesis and body mass in wild Mongolian gerbils (Meriones unguiculatus). Comparative Biochemistry and Physiology A, Integrative and Mo-lecular Physiology, 148 (2):346-353.
Zhang H J, Wang Z K, Zhu W L. 2019. Metabolomics of Eothenomys miletus from five Hengduan Mountains locations in summer. Sci-entific Reports, 9 (1):14924.
Zhao Z J, Zhu Q X, Chen K X, Wang Y K, Cao J. 2013. Energy bud-get, behavior and leptin in striped hamsters subjected to food re-striction and refeeding. PLoS ONE, 8 (1):e54244.
Zhao Z J, Chi Q S, Cao J, Wang D H. 2014. Seasonal changes of body mass and energy budget in striped hamsters:the role of leptin. Physiological & Biochemical Zoology, 87 (2):245-256.
Zhao Z J, Chi Q S, Zhao L, Zhu Q X, Cao J, Wang D H. 2015. Effect of food restriction on energy budget in warm-acclimated striped hamsters. Physiology & Behavior, 147 (4):220-226.
Zhu W L, Jia T, Lian X, Wang Z K. 2008. Evaporative water loss and energy metabolic in two small mammals voles (Eothenomys mile-tus) and mice (Apodemus chevrieri), in Hengduan mountains re-gion. Journal of Thermal Biology, 33 (6):324-331.
Zhu W L, Yang Y H, Jia T, Lian X, Wang Z K, Gong Z D, Guo X G. 2008. Evaporative water loss and body temperature regulation in Eothenomys miletus and Apodemus chevrieri. Acta Theriologica Sinica, 28 (1):65-74. (in Chinese)
Zhu W L, Jia T, Lian X, Wang Z K. 2010. Seasonal variations of maximum metabolic rate in Eothenomys miletus in Hengduan Mountains region. Acta Ecologica Sinica, 30 (5):1133-1139. (in Chinese)
Zhu W L, Jia T, Lian X, Wang Z K. 2010. Effects of cold acclimation on body mass, serum leptin level, energy metabolism and thermo-genesis in Eothenomys miletus in Hengduan Mountains region. Journal of Thermal Biology, 35 (1):41-46.
Zhu W L, Cai J H, Lian X, Wang Z K. 2011. Effects of photoperiod on energy intake, thermogenesis and body mass in Eothenomys miletus in Hengduan Mountain region. Journal of Thermal Biol-ogy, 36 (7):380-385.
Zhu W L, Yang S C, Zhang L, Wang Z K. 2012. Seasonal variations of body mass, thermogenesis and digestive tract morphology in Apodemus chevrieri in Hengduan Mountain region. Animal Biol-ogy, 62 (4):463-478.
Zhu W L, Zhang H, Zhang L, Wang Z K. 2014. Thermogenic proper-ties of Yunnan red-backed voles (Eothenomys miletus) from the Hengduan Mountain region. Animal Biology, 64 (1):59-73.
Zimmermann-Belsing T, Brabant G, Holst J J, Feldt-Rasmussen U. 2003. Circulating leptin and thyroid dysfunction. European Jour-nal of Endocrinology, 149 (4):257-271.
王政昆, 刘璐, 梁子卿,李庆芬, 孙儒泳. 1999. 大绒鼠体温调节和产热特征. 兽类学报, 19 (4):276-286.
王海, 杨晓密, 刘春燕, 王政昆. 2006. 大绒鼠和高山姬鼠的体温调节和产热特征. 兽类学报, 26 (2):144-151.
朱万龙, 杨永宏, 贾婷, 练硝, 王政昆, 龚正达, 郭宪国. 2008. 横断山两种小型哺乳动物的蒸发失水与体温调节. 兽类学报, 28 (1):65-74.
朱万龙, 贾婷, 练硝, 王政昆. 2010. 横断山脉大绒鼠最大代谢率的季节性差异. 生态学报, 30 (5):1133-1139.
张志强, 刘全生, 李纪元, 王德华. 2006. 长爪沙鼠褐色脂肪组织和肝脏产热能力的季节性变化. 动物学报, 52 (6):1034-1041.
罗泽珣, 陈卫, 高武. 2000. 中国动物志. 北京:科学出版社, 89-95.

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外源瘦素注射对昆明和大理地区大绒鼠适应性产热的影响

Effects of exogenous leptin injection on adaptive thermogenesis in Eothenomys miletus between Kunming and Dali regions

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