高原鼢鼠发情周期阴道细胞变化特征及血清促性腺激素含量动态

doi:10.16829/j.slxb.150505

兽类学报 ›› 2021, Vol. 41 ›› Issue (6): 731-740.DOI: 10.16829/j.slxb.150505

• 研究简报 • 上一篇

高原鼢鼠发情周期阴道细胞变化特征及血清促性腺激素含量动态

王艳莉^1,2,3, 姚宝辉^1,2,3, 张彩军^1,2,3, 谭宇尘^1,2,3, 康宇坤^1,2,3, 张德罡^1,2,4, 苏军虎^1,2,3,4

1 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 中美草地畜牧业可持续发展研究中心, 兰州 730070;
2 甘肃农业大学-新西兰梅西大学草地生物多样性研究中心, 兰州 730070;
3 国家林业和草原局高寒草地鼠害防控工程技术研究中心, 兰州 730070;
4 甘肃省祁连山草原生态系统野外科学观测研究站, 天祝 733200

收稿日期:2020-11-28 修回日期:2021-04-26 发布日期:2021-11-12
通讯作者: 苏军虎,E-mail:jhsu_627@126.com
作者简介:王艳莉(1996-),女,硕士研究生,主要从事草地保护研究.
基金资助:
国家自然科学基金（31760706）；甘肃省陇原青年创新创业人才项目（LYRC2018-02）；甘肃省科技计划项目（20JR10RA564）

Characteristics of vaginal cells and dynamics of serum gonadotropin content in estrus cycle of plateau zokor (Eospalax baileyi)

WANG Yanli^1,2,3, YAO Baohui^1,2,3, ZHANG Caijun^1,2,3, TAN Yuchen^1,2,3, KANG Yukun^1,2,3, ZHANG De-gang^1,2,4, SU Junhu^1,2,3,4

1 College of Grassland Science, Key Laboratory of Grassland Ecosystem (Ministry of Education), Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Centers for Grazing land Ecosystem Sustainability, Gansu Agricultural University, Lanzhou 730070, China;
2 Gansu Agricultural University-Massey University Research Centre for Grassland Biodiversity, Gansu Agricultural University, Lanzhou 730070, China;
3 Engineering and Technology Research Center for Alpine Rodent Pest Control, National Forestry and Grassland Administration, Lanzhou 730070, China;
4 Gansu Qilianshan Grassland Ecosystem Observation and Research Station, Tianzhu 733200, China

Received:2020-11-28 Revised:2021-04-26 Published:2021-11-12

摘要/Abstract

关键词: 高原鼢鼠, 发情周期, 阴道细胞涂片, 卵泡刺激素, 黄体生成素

Abstract: In order to determine the change characteristics of vaginal cells in the estrus cycle and the duration of the estrus cycle, vaginal smears and HE staining methods were used to observe and count the types, morphological changes, and proportions of vaginal cells in plateau zokor (Eospalax baileyi). The hormone secretion dynamics of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the serum of plateau zokor in the estrus cycle were determined by ELISA. The results showed that the main vaginal cells of plateau zokor were leukocytes, nuclear epithelial cells, incompletely cornified epithelial cells, and complete cornified epithelial cells. The proportion of nuclear epithelial cells in the proestrus was significantly higher than that during the other three periods (48. 4 ±3. 09)% (n=12, P < 0. 05). The proportion of complete cornified epithelial cells in the oestrus was the highest and significantly higher than that in the other three periods (59. 73 ±7. 59)% (n=15, P < 0. 05). The proportion of leukocytes in the metestrus and dioestrum was significantly higher than that in the proestrus and oestrus (P < 0. 05). The expression level of LH was the highest in the metestrus (4. 709 5 ±1. 094 0) ng/mL (n=6, P < 0. 05), which showed an increasing trend in proestrus and oestrus, and a decreasing trend in the metestrus and dioestrum. There was no significant difference in the expression level of FSH among the four periods (P > 0. 05); the estrus cycle duration of plateau zokor was 16 to 19 d, the characteristics of vaginal cell types in different stages of the estrus cycle were obvious, and the relative number was statistically significant. The study provided a basis for vaginal smear identification in the estrous cycle and reproductive physiology study of plateau zokor.

Key words: Plateau zokor, Estrus cycle, Vaginal cell smear, Follicle-stimulating hormone, Luteinizing hormone

中图分类号:

Q494

王艳莉, 姚宝辉, 张彩军, 谭宇尘, 康宇坤, 张德罡, 苏军虎. 高原鼢鼠发情周期阴道细胞变化特征及血清促性腺激素含量动态[J]. 兽类学报, 2021, 41(6): 731-740.

WANG Yanli, YAO Baohui, ZHANG Caijun, TAN Yuchen, KANG Yukun, ZHANG De-gang, SU Junhu. Characteristics of vaginal cells and dynamics of serum gonadotropin content in estrus cycle of plateau zokor (Eospalax baileyi)[J]. ACTA THERIOLOGICA SINICA, 2021, 41(6): 731-740.

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高原鼢鼠发情周期阴道细胞变化特征及血清促性腺激素含量动态

Characteristics of vaginal cells and dynamics of serum gonadotropin content in estrus cycle of plateau zokor (Eospalax baileyi)

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