基于Cyt b基因和形态学探讨大麝鼩的亚种分化

doi:10.16829/j.slxb.150746

兽类学报 ›› 2023, Vol. 43 ›› Issue (4): 422-429.DOI: 10.16829/j.slxb.150746

基于Cyt b基因和形态学探讨大麝鼩的亚种分化

刘铸¹, 钱洪安¹, 王再山¹, 韩美凤¹, 蔡赫¹, 张春凤², 时艳菁¹, 金志民¹, 陈欢¹, 张隽晟¹

1 牡丹江师范学院生命科学与技术学院, 牡丹江 157011;
2 黑龙江农业经济职业学院, 牡丹江 157041

收稿日期:2022-10-26 修回日期:2023-05-16 发布日期:2023-08-01
通讯作者: 刘铸,E-mail:liuzhu590@sohu.com
作者简介:刘铸(1979-)，男，教授，博士，主要从事动物保护及分子生态学研究.
基金资助:
黑龙江省自然科学基金(LH2021C095)；黑龙江省省属高等学校基本科研业务费科研项目(1451PT008,1452TD008,1451TD002,1354MSYTD007)；牡丹江师范学院科研项目(GP2022009,QN2021006)

Discussion on subspecies differentiation of Crocidura lasiura based on Cyt b gene and morphology

LIU Zhu¹, QIAN Hongan¹, WANG Zaishan¹, HAN Meifeng¹, CAI He¹, ZHANG Chunfeng², SHI Yanjing¹, JIN Zhimin¹, CHEN Huan¹, ZHANG Junsheng¹

1 College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157011, China;
2 Hei Long Jiang Agricultural Economics Vocational College, Mudanjiang 157041, China

Received:2022-10-26 Revised:2023-05-16 Published:2023-08-01

摘要/Abstract

摘要： 为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题，在中国东北地区采集大麝鼩标本13号，结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示：在绝大多数个体中，分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长：> 82 mm vs.< 82 mm，尾长：> 39 mm vs.< 39 mm，颅基长：> 20 mm vs.< 20 mm)；中国东北—俄罗斯远东种群体背毛色更黑，韩国种群体背毛色更灰；中国东部种群尾较长，绝大多数超过体长之半；而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析，都显示大麝鼩样本可分为3个地理谱系：中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种：东北亚种(C.l.la-siura)分布于中国东北和俄罗斯远东，韩国亚种(C.l.thomasi)分布于韩国，华东亚种(C.l.campus-lincolnensis)分布于中国东部。

关键词: 麝鼩属, 大麝鼩, 亚种, Cyt b基因, 形态学

Abstract: In order to clarify the subspecies differentiation of Crocidura lasiura, this study analyzed 13 specimens of this species collected from Northeast China together with morphological data and Cyt b gene sequences. Morphological results showed that C. lasiura from Northeast China—Far East Russia is larger than that from South Korea (head-and-body length: > 82 mm vs. < 82 mm, tail length: > 39 mm vs. < 39 mm, condylobasal length of skull: > 20 mm vs. < 20 mm) in the majority of individuals. The dorsum of the body is darker in individuals from Northeast China-Far East Russia, while it is grayer in South Korean shrews. Specimens from East China exhibit a longer tail (the tail is more than half the headand-body length) in the majority of individuals. On the other hand, the tail length of C. lasiura from Northeast China, Far East Russia, and South Korea is less than half of head-and-body length in the majority of individuals. The analysis of genetic distance, phylogeny, and median-joining network based on Cyt b gene all supported that the samples of C. lasiura were divided into three geographical lineages: one from Northeast China-Far East Russia, other from South Korea, and the third from East China. The results supported that C. lasiura can be divided into three subspecies: C. l. lasiura in Northeast China and Far East Russia, C. l. thomasi in South Korea, and C. l. campus-lincolnensis in East China.

Key words: Crocidura, Crocidura lasiura, Subspecies, Cyt b gene, Morphology

中图分类号:

Q959

刘铸, 钱洪安, 王再山, 韩美凤, 蔡赫, 张春凤, 时艳菁, 金志民, 陈欢, 张隽晟. 基于Cyt b基因和形态学探讨大麝鼩的亚种分化[J]. 兽类学报, 2023, 43(4): 422-429.

LIU Zhu, QIAN Hongan, WANG Zaishan, HAN Meifeng, CAI He, ZHANG Chunfeng, SHI Yanjing, JIN Zhimin, CHEN Huan, ZHANG Junsheng. Discussion on subspecies differentiation of Crocidura lasiura based on Cyt b gene and morphology[J]. ACTA THERIOLOGICA SINICA, 2023, 43(4): 422-429.

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基于Cyt b基因和形态学探讨大麝鼩的亚种分化

Discussion on subspecies differentiation of Crocidura lasiura based on Cyt b gene and morphology

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