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石斌山, 诸葛阳
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SHI Binshan, ZHUGE Yang
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摘要: 用聚丙烯酰胺凝胶薄层(o.5毫米)等电聚焦电泳分析了臭鼩(Suncus m.murinus) 心肌、骨骼肌、肾脏、脾脏、肝脏和脑6种组织器官的酯酶和苹果酸脱氢酶同工酶。结果表明臭鼩6种组织的酯酶同工酶分别具有11—24条酶带,存在着明显的组织特异性。实验还发现其酯酶同工酶存在异型酶。臭鼩6种组织的苹果酸脱氢酶同工酶则未发现存在明显的组织特异性。
关键词: 臭鼩, 酯酶, 苹果酸脱氢酶, 薄层等电聚焦, 异型酶, 组织特异
Abstract: Es and MDH isozymes of six kinds of tissues in Suncus murinus were analysed byisoelectrie focusing in thin layer of polyacrylamide gel.There were 11-24 bands of Es isozymes in the tissues of heart,muscle,kidney,spleen,liver,and brain separately.Difference of Es zymograms between tissues were also found.The allozymes of Es isozymes were detected in the experiment.The eletrophoretograms of MDH isozymes in different tissues were similar except that in spleen.There were 1-2 m-MDH and L-2 s-MDH bands Of MDH isozymes.
Key words: Suncus murinus, Es (Esterase), MDH (Malate dehydrogenase), thin layer isoelectric focusing, allozyme, tissue specifity
石斌山, 诸葛阳. 臭鼩酯酶和苹果酸脱氢酶同工酶的电泳研究[J]. .
SHI Binshan, ZHUGE Yang. ELECTROPHORETIC STUDIES ON THE ESTERASE AND MALATE DEHYDROGENASE ISOZYMES OF TISSUES FROM SUNCUS MURINUS[J]. .
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https://www.mammal.cn/CN/Y1993/V13/I1/25