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中华姬鼠与大林姬鼠的同工酶差异

傅必谦, 陈卫,高武,张可心,袁虹,芮朝胜,李举怀   

  1. 首都师范大学生物系
  • 出版日期:2006-06-29 发布日期:2008-07-07

INTERSPECIFIC OF ISOZYME SYSTEMS BETWEEN APODEMUS DRACO AND APODEMUS PENINSULAE

FU Biqian,CHEN Wei,GAO Wu,ZHANG Kexin,YUAN Hong,RUI Chao sheng,LI Juhuai   

  • Online:2006-06-29 Published:2008-07-07

摘要:  中华姬鼠(Apodemus draco) 和大林姬鼠(Apodemus peninsulae) 是形态学上十分相似的两种鼠类。为了对两种姬鼠的分类提供生物化学方面的依据, 采用聚丙烯酰胺凝胶等电聚焦电泳方法比较和分析了两种姬鼠的LDH 同工酶、EST 同工酶和SOD 同工酶的差异。结果表明, 两种姬鼠的LDH 同工酶酶谱基本相似, 而EST 同工酶和SOD 同工酶酶谱则存在明显的种间差异。根据EST 同工酶A2带的有无和SOD 同工酶主带等电点的差别, 能将两种姬鼠很容易区分开来。

关键词: 中华姬鼠, 大林姬鼠, LDH 同工酶, EST 同工酶, SOD 同工酶, 生化分类

Abstract: Apodemus draco and A . peninsulae are the two species quite similar in morphological characters. In order to explore some diagnostic means of the biochemical taxonomy of the two species, LDH, EST and SOD isozymes were isolated from legmuscles, breastmuscles, hearts,kidneys, livers, spleens, lungs and brains of A . d raco and A . peninsulae and were analyzed by isoelectric focusing with PAGE. D istinctinterspecific differences were found in EST and OD electrophoregrams, but not found in LDH electrophoregrams. It was suggested that A .d raco and A . peninsulae could be unequivocally distinguished by the presence or absence of A 2 band in EST isozymes or by the variations of isoelectric point (pI) of main bands in SOD isozymes.

Key words: Apodemus draco, Apodemus peninsulae, EST isozymes, LDH isozymes, SOD isozymes, Biochemical taxonomy