关键词: 鲸类, C-mos 基因, 变异, 系统发生

Abstract: Primers for amplifying the cetacean c-mos genes were designed based on the alignment of conservative region of c-mos genes from pigs and some other mammals. With these primers , the partial coding region of cetacean c-mos gene , which was 546 base pairs (bp) in length , was amplified by using polymerase chain reaction (PCR) for 23 toothed whales belonging to 12 species of five families. The amplified products were purified and directly sequenced by an ABI 310 automated genetic analyzer. The results suggested a very low level of sequence variation at cetacean c-mos genes. In the phylogenetic analysis using maximum likelihood method incorporated in computer software PHYLIP , species from the same family constituted respective monophyletic groups , but the relationship at subfamily level was not well resolved. This suggested that c-mos gene is an appropriate candidate in the phylogenetic study only at family andPor higher level.

Key words: Cetaceans, C-mos gene, Variability, Phylogeny