关键词: 东北马鹿, 粪球形态, SRY 基因, 性别鉴定

Abstract: The DNA sex of wild wapiti was determined by PCR amplification of the SRY genes of 76 feces samples. Among
of them,49 samples were identified as male,and the others as female. All fecal pellets presented one of two shapes:bullet-like and jujube-stone like. The bullet-like fecal pellets displayed a short thick form with a low length/ width ratio. Thejujube-stone like ones displayed a thin long form with high length/ width ratio. Using these shapes,the 76 samples were divided into two groups:51 bullet-like and 25 jujube-stone like feces. All the samples were clustered by the ratio of averaged length and width,and then the discrimination equation was established. The statistical results showed that the consistent degree of sex determination by fecal statistic index and DNA method was 79. 71% ,while morphological method and real sex was 90. 56% . So,the bullet-like pellets are from male wapiti,and the jujube-stone like ones from females. Our results show that wapiti sex can be directly determined by its pellet shape in the wild. At the same time,the discrimination equation could be used as associated method.

Key words: Cervus elephus xanthopygus, Pellet shape, Sex determination, SRY gene