关键词: 双峰驼, 卵泡发育, 卵泡液, 蛋白质组学分析, 双向电泳

Abstract: The aim was to identify differentially expressed proteins in the follicular fluid. Follicles were collected from the Bactrian camel (Camelus bactrianus)and divided into six groups according to their diameter,and crude follicular fluid samples were analyzed by 2 - dimensional gel electrophoresis (2 - DE). Differential protein spots were detected by
PDQuest 8 0 software after staining with commassie blue. Thirteen differentially expressed proteins were found from the six classes of developmental follicles. These spots were identified by LC-MS/ MS. Seven unique proteins was found,including hemoglobin,toll-like receptor 9,antithrombin,recombination activating protein 1, polyubiquitin, transmembrane and coiled coil domain protein 3,and gamma fibrinogen. Based on existing knowledge of their functions and patterns of expression, we hypothesize that toll-like receptor 9,polyubiquitin,gamma fibrinogen and recombination activating protein 1 might play significant roles in the development and maturation of oocytes,and ovarian follicular growth and development. This study provides a solid basis for further research on the processes and mechanisms which regulate follicular development and oocyte maturation in Bactrian camel

Key words: Camelus bactrianus, Follicular development, Follicular fluid, Proteomic analysis, 2 - DE