MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达

doi:10.16829/j.slxb.150605

兽类学报 ›› 2022, Vol. 42 ›› Issue (4): 410-419.DOI: 10.16829/j.slxb.150605

MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达

安康¹^,²^,³, 包明芳¹^,²^,³, 姚宝辉¹^,²^,³, 康宇坤¹^,²^,³, 谭宇尘¹^,²^,³, 王艳莉¹^,²^,³, 苏军虎¹^,²^,³()

^1.甘肃农业大学草业学院，草业生态系统教育部重点实验室，中美草地畜牧业可持续发展研究中心，兰州 730070
^2.甘肃农业大学—新西兰梅西大学草地生物多样性研究中心，兰州 730070
^3.甘肃省祁连山草原生态系统野外科学观测研究站，天祝 733200

收稿日期:2021-09-02 接受日期:2022-02-23 出版日期:2022-07-30 发布日期:2022-07-22
通讯作者: 苏军虎
作者简介:安康 (1997- )，男，硕士研究生，主要从事草地保护研究.
基金资助:
国家自然科学基金(31760706);甘肃省陇原青年创新创业人才项目(LYRC2018-02);甘肃省草原生态修复治理科技支撑(LCJ2021020)

Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non‑breeding seasons

Kang AN¹^,²^,³, Mingfang BAO¹^,²^,³, Baohui YAO¹^,²^,³, Yukun KANG¹^,²^,³, Yuchen TAN¹^,²^,³, Yanli WANG¹^,²^,³, Junhu SU¹^,²^,³()

^1.College of Grassland Science, Key Laboratory of Grassland Ecosystem (Ministry of Education), Pratacultural Engineering Laboratory of Gansu Province, Sino–U. S. Centers for Grazing Land Ecosystem Sustainability, Gansu Agricultural University, Lanzhou 730070, China
^2.Gansu Agricultural University–Massey University Research Centre for Grassland Biodiversity, Gansu Agricultural University, Lanzhou 730070, China
^3.Gansu Qilianshan Grassland Ecosystem Observation and Research Station, Tianzhu 733200, China

Received:2021-09-02 Accepted:2022-02-23 Online:2022-07-30 Published:2022-07-22
Contact: Junhu SU

摘要/Abstract

摘要：

高原鼢鼠 (Eospalax baileyi) 终年营地下生活，感光受洞道限制，但褪黑素 (Melatonin) 分泌水平仍存有季节差异，为探明褪黑素对高原鼢鼠季节性繁殖的调控作用，研究利用q?PCR技术检测雄性高原鼢鼠繁殖期 (5月) 和非繁殖期 (9月) 下丘脑、垂体及睾丸中褪黑素受体1a (Melatonin receptor 1a, MTNR1a) 和褪黑素受体1b (Melatonin receptor 1b, MTNR1b) 基因mRNA的相对表达量，通过免疫组织化学技术对MTNR1a和MTNR1b在睾丸中定位，并采用Image Pro Plus软件进行免疫组化阳性评价。结果发现，高原鼢鼠繁殖期下丘脑和垂体中MTNR1a基因的相对表达量显著高于非繁殖期的相对表达量 (P < 0.05)，MTNR1b基因的相对表达量在不同时期无显著差异 (P > 0.05)，但非繁殖期睾丸中MTNR1a和MTNR1b基因的相对表达量均显著高于繁殖期 (P < 0.01)；繁殖期除长形精子外的所有类型细胞以及非繁殖期的间质细胞、支持细胞和精原细胞中均观察到MTNR1a的阳性信号，繁殖期除精原细胞和长形精子细胞外的所有类型细胞，以及非繁殖期间质细胞和支持细胞中均观察到MTNR1b的阳性信号，且非繁殖期MTNR1a和MTNR1b的平均光密度值均显著高于繁殖期 (P < 0.01)。MTNR1a和MTNR1b基因在雄性高原鼢鼠HPG轴上的表达模式，提示了褪黑素在其季节性繁殖调控中的潜在作用。

关键词: 高原鼢鼠, MTNR1a基因, MTNR1b基因, 季节性繁殖

Abstract:

The plateau zokor (Eospalax baileyi) is an endemic subterranean rodent species with limited exposure light. Previous study has shown seasonal differences in levels of melatonin secretion in plateau zokors. However, the expression pattern of melatonin receptor genes on hypothalamic?pituitary?gonadal (HPG) axis is not clear. In this study, q–PCR was employed to detect the relative expression levels of genes mRNA of melatonin receptor 1a (MTNR1a)and melatonin receptor 1b (MTNR1b) in hypothalamus, pituitary, and testis of male plateau zokors during the breeding (May) and non?breeding seasons (September). The location of MTNR1a and MTNR1b in testis was determined by immunohistochemical technology, and immunohistochemical positive evaluation was conducted in Image Pro Plus software. The results showed that the relative expression levels of MTNR1a in hypothalamus and pituitary were significantly higher during the breeding season (P < 0.05), while there was no significant difference in the relative expression levels of MTNR1b in different seasons (P > 0.05). The relative expression levels of MTNR1a and MTNR1b in testis during the non?breeding season were higher than that during the breeding season (P < 0.01). The positive signal of MTNR1a in testis was observed in all types of cells except elongated spermatid during the breeding season and in Leydig cells, Sertoli cells, and spermatogonia during the non?breeding season. The positive signal of MTNR1b in testis was observed in all types of cells except spermatogonia and elongated spermatid during the breeding season and in Leydig cells and Sertoli cells during the non?breeding season. The average optical density values of immunopositive signals in MTNR1a and MTNR1b during the non?breeding season were significantly higher compared with the breeding season (P < 0.01). The expression patterns of MTNR1a and MTNR1b on the HPG axis of male plateau zokor during the breeding and the non?breeding seasons revealed the potential role of melatonin in seasonal reproduction regulation.

Key words: Plateau zokor, MTNR1a gene, MTNR1b gene, Seasonal reproduction

中图分类号:

Q753

安康, 包明芳, 姚宝辉, 康宇坤, 谭宇尘, 王艳莉, 苏军虎. MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达[J]. 兽类学报, 2022, 42(4): 410-419.

Kang AN, Mingfang BAO, Baohui YAO, Yukun KANG, Yuchen TAN, Yanli WANG, Junhu SU. Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non‑breeding seasons[J]. ACTA THERIOLOGICA SINICA, 2022, 42(4): 410-419.

图/表 5

表1 雄性高原鼢鼠MTNR1a和MTNR1b引物序列

Table 1 Primers sequence for MTNR1a and MTNR1b of male plateau zokor

基因

Genes

引物序列

Primer sequence

退火温度

Annealing temperature （℃）

片段大小

Size （bp）

MTNR1a

F： 5´–TCTTCAGGTCAGATGGACGGT–3´

R： 5´–CCCCCAGCAAATGGCAAAAA –3´

53.5

125

MTNR1b

F： 5´–ACCCCTGTGGCAGCACAGTG –3´

R： 5´–CTGAGGGACGTATTCCTTTGC –3´

53.5

156

β–actin

F： 5´–TTGTGCGTGACATCAAAGAG –3´

R： 5´–ATGCCAGAAGATTCCATACC –3´

53.5

200

表2 高原鼢鼠繁殖期和非繁殖期性腺器官重量变化

Table 2 Weight changes of gonad organs during the breeding and non?breeding seasons of plateau zokor

时期

Period

个体数

Number

体重

Body mass （g）

睾丸重

Testis weight （g）

附睾重

Epididymis weight （g）

精囊腺重

Seminal vesicle weight （g）

繁殖期

Breeding

非繁殖期

Non‑breeding

313.36 ± 7.09

0.12 ± 0.01

0.04 ± 0.01

图1 MTNR1a和MTNR1b mRNA在繁殖期与非繁殖期高原鼢鼠下丘脑、垂体和睾丸中的表达. A：MTNR1a mRNA的相对表达量；B：MTNR1b mRNA的相对表达量. *P < 0.05；**P < 0.01

Fig. 1 MTNR1a and MTNR1b mRNA in the hypothalamus, pituitary, and testis of plateau zokor during the breeding and non?breeding seasons. A: relative expression level of MTNR1a mRNA; B: relative expression level of MTNR1b mRNA. *P < 0.05; **P < 0.01

图2 高原鼢鼠繁殖期与非繁殖期睾丸中MTNR1a和MTNR1b的免疫组织化学染色. A、D：MTNR1a的免疫组织化学染色；B、E：MTNR1b的免疫组织化学染色；C、F：对照组 (用PBS代替第一抗体)；SC：支持细胞；LC：间质细胞；Spg：精原细胞；pSpc：初级精母细胞；sSpc：次级精母细胞；rSpd：圆形精子细胞；eSpd:：长形精子细胞

Fig. 2 Immunohistochemical staining of MTNR1a and MTNR1b in testicular of plateau zokor during the breeding and non?breeding seasons. A, D: immunohistochemical staining of MTNR1a; B, E: immunohistochemical staining of MTNR1b; C, F: control group (using PBS instead of the first antibody); SC: Sertoli cell; LC: Leydig cell; Spg: spermatogonia; pSpc: primary spermatocyte; sSpc: secondary spermatocyte; rSpd: round spermatid; eSpd: elongate spermatid

图3 MTNR1a (A) 和MTNR1b (B) 在繁殖期与非繁殖期高原鼢鼠睾丸中免疫阳性信号的平均光密度值.**P < 0.01

Fig. 3 The average optical density of immunopositive signal in MTNR1a (A) and MTNR1b (B) of plateau zokor testis during the breeding and the non?breeding seasons. **P < 0.01

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MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达

Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non‑breeding seasons

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