关键词: 水貂, 自咬症, RAPD, SCAR, 分子标记

Abstract:

DNA samples obtained from healthy and self-biting Jinzhou Black minks were amplified by PCR with 100 random primers. A DNA fragment corresponding to self-biting cases was identified and marked as S356. The DNA fragment was purified and cloned into PMD18-T vector, subsequently, transformed into E. Coli JM109 and sequenced. We applied the SCAR label technology to detect the molecular marker S356 among the healthy and self-biting minks. Based on the sequence of S356, two pairs of SCAR primers,S356 - 8001 and S356 - 8002,were designed and used in PCR detection. The result showed that the target DNA fragment was amplified from 29 of 30 self-biting minks (96.67% positive correspondence rate) with the SCAR labeling primers S356 - 8001 and S356 -8002. However, there was no target fragment detected from healthy minks. The DNA fragment was denominated as DS356800, which could be used as a genetic marker to differentiate potential self-biting individuals from healthy minks, and could form a guideline for future anti-disease genetic breeding to eliminate the self-biting disease from mink populations.

Key words: Mink (Mustla vison), Molecular marking, RAPD, Self-biting, SCAR