关键词: 化学通讯, 化学成分, 四川短尾鼩, 尿液

Abstract: Urine plays an important role in chemical communications among mammals. In this study，headspace-soild phase microextraction(HS-SPME) and gas chromatography-mass spectrometry(GC-MS) were used to analyze the urine compositions in Anourosorex squamipes. Our reserach showed that 1) Through searching the mass spectrometry library of the National Institute of Standards and Technology(NIST)，we found that the 86 chemical compounds of their urine mainly included toaldehydes，alcohols，ketones，heterocycles，esters，hydrocarbons，aromatic hydrocarbons，sulfurcompounds，olefins，phenols，etc，and different individual odors contained unique volatile compounds. 2) Ten volatile compounds in urine were specific to male and female individuals. Six volatile compounds in urine were found only in females while four compounds were found only in males.This indicates that there are significant sexual differences in the chemical compositions of urine.There are 76 volatile substances in the urine of the female while only 33 in the urine of the male，also indicating that females had more chemical signals in urine than males. 3)The relative contents of different chemical components in urine were different，and the relative contents of the same component among different individuals were different. By analyzing the chemical composition of the urine of Anourosorex squamipes，This study will further our understanding of the chemical communication function of volatile components in the urine of Anourosorex squamipes，and to provide basic information for the development of attractants.

Key words: Chemical communication, Volatile constituents, Anourosorex squamipes, Urine