宁夏贺兰山岩羊小反刍兽疫疫情来源分析

doi:10.16829/j.slxb.150864

兽类学报 ›› 2024, Vol. 44 ›› Issue (4): 497-505.DOI: 10.16829/j.slxb.150864

宁夏贺兰山岩羊小反刍兽疫疫情来源分析

尹才^1,2, 李知新^2,3, 李林⁴, 邸静^1,2, 周海宁², 张玉玲², 白涛涛², 王玉梅², 赵源⁵, 林邱雄^2,3, 吴亚文², 马龙², 李莉娟², 王晓亮^2,3, 曾巧英¹

1 甘肃农业大学动物医学院, 兰州 730070;
2 宁夏回族自治区动物疾病预防控制中心, 银川 750011;
3 宁夏大学农学院, 银川 750021;
4 中国动物卫生与流行病学中心, 青岛 266033;
5 贺兰县动物疾病预防控制中心, 贺兰 750200

收稿日期:2023-10-08 修回日期:2024-01-29 出版日期:2024-07-30 发布日期:2024-08-03
通讯作者: 李知新，E-mail:goldenapple_2001@163.com;曾巧英，E-mail:zengqiaoying@gsau.edu.cn
作者简介:尹才(1988-)，男，博士研究生，主要从事动物疫病、检测、诊断方面的研究.E-mail:yincai22@163.com
基金资助:
宁夏青年拔尖人才“自治区学术技术带头人”后备人选人才项目；自治区动物疫病净化科技创新团队项目(2020CXTDLX05)

Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain, Ningxia, China

YIN Cai^1,2, LI Zhixin^2,3, LI Lin⁴, DI Jing^1,2, ZHOU Haining², ZHANG Yuling², BAI Taotao², WANG Yu-mei², ZHAO Yuan⁵, LIN Qiuxiong^2,3, WU Yawen², MA Long², LI Lijuan², WANG Xiaoliang^2,3, ZENG Qiaoying¹

1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2 Ningxia Hui Autonomous Center for Animal Disease Control and Prevention, Yinchuan 750011, China;
3 School of Agriculture, Ningxia University, Yinchuan 750021, China;
4 China Animal Health and Epidemiology Center, Qingdao 266033, China;
5 Helan County Center for Animal Disease Control and Prevention, Helan 750200, China

Received:2023-10-08 Revised:2024-01-29 Online:2024-07-30 Published:2024-08-03

摘要/Abstract

摘要： 2018—2023年，宁夏贺兰山国家级自然保护区连续发现多起野生岩羊(Pseudois nayaur)不明原因死亡，通过解剖采样、实时荧光RT-PCR检测，证实为小反刍兽疫病毒(PPRV)核酸阳性。使用Vero细胞分离出了部分毒株，采用RT-PCR技术对宁夏地区病死家养羊和野生动物病料组织扩增PPRV F基因的全长片段。使用DNA-STAR Lasergene和MEGA X软件对岩羊PPRV F基因序列进行比对和分析，构建系统发育树分析其分子遗传特征。结果显示：宁夏地区不同年度家养羊和贺兰山岩羊感染的PPRV毒株F基因同源性均在99%以上，其中2023年毒株与2018年、2019年1月毒株同源性最高，为99.8%，与2014年、2016年宁夏家养羊PPR疫情毒株及China/XJYL/2013(KM091959.1)同源性为99.6%。遗传进化分析表明，宁夏野生岩羊及家养羊PPRV同源性较高，均属于基因Ⅳ系中亚分支。因此推测宁夏及周边省份流行的PPR疫情可能通过不同途径在贺兰山野生动物种群中扩散蔓延，需加强PPR等野生动物疫源疫病监测。

关键词: 宁夏贺兰山, 岩羊, 小反刍兽疫, 分子遗传特征

Abstract: From 2018 to 2023, wild Pseudois nayaur were found to have died of unknown causes continuously in Helan Mountain National Nature Reserve of Ningxia, which was confirmed to be positive for peste des petits ruminants virus (PPRV) nucleic acid through anatomical sampling and real-time fluorescent RT-PCR detection. Vero cells were used to isolate some strains of PPRV, and RT-PCR was used to amplify the full-length fragments of PPRV F gene from diseased domestic sheep and wild animal samples in Ningxia. DNASTAR Lasergene and MEGA X were used to compare and analyze the PPRV F gene sequence and construct a phylogenetic tree to analyze its molecular genetic characteristics. The results show: the homology of F gene of PPRV strains infected by domestic sheep and Pseudois nayaur of Helan Mountain in different years in Ningxia was above 99%, among which the 2023 strain had the highest homology of 99. 8% with that in January 2018 and 2019, and 99. 6% with the PPR epidemic strains in 2014 and 2016 in Ningxia and China/XJYL/2013(KM091959. 1) of Xinjiang. Genetic analysis showed that the PPRV of wild Pseudois nayaur and domestic sheep in Ningxia belonged to the central Asian branch of gene Ⅳ line. Therefore, it is speculated that the epidemic of PPR in Ningxia and neighboring provinces may have spread in the wild animals of Helan Mountain through different ways, and it is necessary to strengthen the surveillance of PPR and other wild animal diseases.

Key words: Helan Mountain in Ningxia, Pseudois nayaur, Peste des petits ruminants, Molecular genetic characteristics

中图分类号:

Q938

尹才, 李知新, 李林, 邸静, 周海宁, 张玉玲, 白涛涛, 王玉梅, 赵源, 林邱雄, 吴亚文, 马龙, 李莉娟, 王晓亮, 曾巧英. 宁夏贺兰山岩羊小反刍兽疫疫情来源分析[J]. 兽类学报, 2024, 44(4): 497-505.

YIN Cai, LI Zhixin, LI Lin, DI Jing, ZHOU Haining, ZHANG Yuling, BAI Taotao, WANG Yu-mei, ZHAO Yuan, LIN Qiuxiong, WU Yawen, MA Long, LI Lijuan, WANG Xiaoliang, ZENG Qiaoying. Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain, Ningxia, China[J]. ACTA THERIOLOGICA SINICA, 2024, 44(4): 497-505.

参考文献

Aguilar X F, Fine A E, Pruvot M, Njeumi F, Walzer C, Kock R, Shiileg damba E. 2018. PPR virus threatens wildlife conservation. Sci ence, 362 (6411):165-166.
Aguilar X F, Mahapatra M, Begovoeva M, Kalema-Zikusoka G, Driciru M, Ayebazibwe C, Adwok D S, Kock M, Lukusa J P K, Muro J, Marco I, Colom-Cadena A, Espunyes J, Meunier N, Ca bezón O, Caron A, Bataille A, Libeau G, Parekh K, Parida S, Kock R. 2020. Peste des petits ruminants at the wildlife -livestock interface in the Northern Albertine Rift and Nile Basin, East Africa. Viruses, 12 (3):293.
Balamurugan V, Sen A, Venkatesan G, Bhanot V, Yadav V, Bhanu prakash V, Singh R K. 2012. Peste des petits ruminants virus de tected in tissues from an Asiatic lion (Panthera leo persica) be longs to Asian lineage Ⅳ. Journal of Veterinary Science, 13 (2):203-206.
Banyard A C, Parida S, Batten C, Oura C, Kwiatek O, Libeau G. 2010. Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. Journal of General Virology, 91 (12):2885-2897.
Dong D D, Liu T, Miao Q H, Zhu J, Zhang L, Yin D D, Tang J Y, Li C F, Chen Z Y, Liu G Q. 2019. Molecular biology and vaccine de velopment of peste des petits ruminants virus. Chinese Journal of Animal Infectious Diseases, 27 (1):110-118. (in Chinese)
Gao S, Xu G, Zeng Z, Lv J, Huang L, Wang H, Wang X. 2021. Transboundary spread of peste des petits ruminants virus in western China:a prediction model. PLoS ONE, 16 (9):e0257898.
Hodgson S, Moffat K, Hill H, Flannery J T, Graham S P, Baron M D, Darpel K E, Heise M T. 2018. Comparison of the immunogenici ties and cross-lineage efficacies of live attenuated peste des petits ruminants virus vaccines PPRV/Nigeria/75/1 and PPRV/Sungri/96. Journal of Virology, 92 (24):e01471-18.
Kan W, Li L B, Zhao X Y, Yang L, Sun Y, Cai J S. 2023. Diagnosis of peste des petits ruminants and genetic evolution analysis of N and F genes of Pseudois nayaur in Qinghai. Acta Theriologica Si nica, 43 (2):171-181. (in Chinese)
Kinimi E, Odongo S, Muyldermans S, Kock R, Misinzo G. 2020.Paradigm shift in the diagnosis of peste des petits ruminants:scop ing review. Acta Veterinaria Scandinavica, 62 (1):7.
Liu F, Li J, Li L, Liu Y, Wu X, Wang Z. 2018. Peste des petits rumi nants in China since its first outbreak in 2007:a 10-year review.Transboundary and Emerging Diseases, 65 (3):638-648.
Liu F B, Huang S M, Xie J C, Liang J, Su Y, Wang X Q, Wang Z B, Xu J G, Zhang J W, Shi S, Teng L W, Liu Z S. 2023. Population sta tus and protection of blue sheep in Helan Mountains of the Inner Mongolia, China. Acta Ecologica Sinica, 43 (14):5829-5839.(in Chinese)
Pruvot M, Fine A E, Hollinger C, Strindberg S, Damdinjav B, Buu veibaatar B, Chimeddorj B, Bayandonoi G, Khishgee B, Sandag B, Narmandakh J, Jargalsaikhan T, Bataa B, McAloose D, Shatar M, Basan G, Mahapatra M, Selvaraj M, Parida S, Njeumi F, Kock R, Shiilegdamba E. 2020. Outbreak of peste des petits ruminants among critically endangered Mongolian saiga and other wild un gulates, Mongolia, 2016-2017. Emerging Infectious Diseases, 26(1):51-62.
Sun G, Zhang H, Wei Q, Zhao C, Yang X, Wu X, Xia T, Liu G, Zhang L, Gao Y, Sha W, Li Y. 2019. Comparative analyses of fecal mi crobiota in European mouflon (Ovis orientalis musimon) and blue sheep (Pseudois nayaur) living at low or high altitudes. Frontiers in Microbiology, 10:1735.
Xie J C, Meng D H, Li Z Z, Zhang Z R, Liu Z S, Teng L W. 2022.Population quantity and structure of Pseudois nayaur in Helan Mountain National Nature Reserve, Ningxia. Acta Ecologica Si nica, 42 (10):4189-4196. (in Chinese)
Yang X F. 2013. The study of F protein immunogenicity and interac tion of F and H protein of PPRV. Master thesis. Lanzhou:Gansu Agriculture University. (in Chinese)
Ying L, Yang Y Q, Li L, Zhao Q B, Wang X Z, La H, Liu F X, Tan S K, Song L Z, Wu X D, Cai J S. 2019. Diagnosis and control of the first case of peste des petits ruminants in sheep in Qinghai Pla teau. Animal Husbandry and Veterinary Medicine, 51 (12):112-117. (in Chinese)
Zakian A, Nouri M, Kahroba H, Mohammadian B, Mokhber-Dezfouli M R. 2016. The first report of peste des petits ruminants (PPR)
in camels (Camelus dromedarius) in Iran. Tropical Animal Health and Production, 48 (6):1215-1219.
Zhou H N. 2018. Surveillance and control of peste des petits rumi nants in sheep and goats Ningxia. Master thesis. Yangling:North west Agriculture and Forestry University. (in Chinese)
刘付宾, 黄师梅, 谢建冲, 梁军, 苏云, 王晓勤, 王兆锭, 徐建国, 张继武, 时光, 滕丽微, 刘振生. 2023. 内蒙古贺兰山冬春季岩羊种群现状及保护. 生态学报, 43 (14):5829-5839.
杨香芳. 2013. 小反刍兽疫病毒 F 蛋白与 H 蛋白相互作用及 F 蛋白免疫原性研究. 兰州:甘肃农业大学硕士学位论文.
应兰, 杨毅青, 李林, 赵全邦, 王谢忠, 拉华, 刘拂晓, 谭生魁, 宋龙智, 吴晓东, 蔡金山. 2019. 青海高原首例岩羊小反刍兽疫诊断与控制. 畜牧与兽医, 51 (12):112-117.
周海宁. 2018. 宁夏羊小反刍兽疫疫情监测及防控研究. 杨凌:西北农林科技大学硕士学位论文.
董丹丹, 刘腾, 缪秋红, 朱杰, 张莉, 殷冬冬, 唐井玉, 李传峰, 陈宗艳, 刘光清. 2019. 小反刍兽疫病毒分子生物学及其疫苗研究进展. 中国动物传染病学报, 27 (1):110-118.
谢建冲, 孟德怀, 李宗智, 张致荣, 刘振生, 滕丽微. 2022. 宁夏贺兰山国家级自然保护区岩羊 (Pseudois nayaur) 种群数量及结构.生态学报, 42 (10):4189-4196.
阚威, 李雷斌, 赵旭阳, 杨林, 孙雨, 蔡金山. 2023. 青海岩羊源小反刍兽疫病毒 N、F 基因的遗传进化分析. 兽类学报, 43 (2):171-181.

[1]	阚威, 李雷斌, 赵旭阳, 杨林, 孙雨, 蔡金山. 青海岩羊源小反刍兽疫病毒N、F基因的遗传进化分析[J]. 兽类学报, 2023, 43(2): 171-181.
[2]	迟翔文江峰高红梅覃雯吴彤张婧捷蔡振媛张同作. 三江源国家公园雪豹和岩羊生境适宜性分析[J]. 兽类学报, 2019, 39(4): 397-409.
[3]	苏梦雨张同作连新明苏建平都玉蓉. 利用角形几何特征和计算机视觉技术确定岩羊年龄的新方法[J]. 兽类学报, 2019, 39(3): 234-241.
[4]	王芮高惠滕丽微刘振生. 宁夏贺兰山岩羊肌肉营养成分分析[J]. 兽类学报, 2018, 38(4): 352-358.
[5]	申定健, 王淯, 格来, 曾国伟, 黄艺川, 李开俊, 唐伦. 四川省竹巴笼自然保护区矮岩羊的集群特征[J]. , 2009, 29(3): 239-245.
[6]	刘延德, 周材权,黄成思, 胡锦矗. 岩羊和矮岩羊头骨形态特征比较[J]. , 2007, 27(4): 376-379.
[7]	李新庆, 刘振生, 王小明, 崔多英, 李志刚, 胡天华. 发情交配期贺兰山岩羊的集群特征[J]. , 2007, 27(1): 39-44.
[8]	曹丽荣刘振生王小明胡天华翟昊侯建海. 贺兰山保护区冬季岩羊集群特征的初步分析[J]. , 2005, 25(2): 200-204.
[9]	刘振生,曹丽荣,王小明,李涛,李志刚. 贺兰山岩羊冬季对卧息地的选择[J]. , 2005, 25(1): 1-8.
[10]	余玉群　郭松涛　白庆生　李志刚　胡天华　吕海军. 贺兰山岩羊种群结构的季节性变化[J]. , 2004, 24(3): 200-204.
[11]	曹丽荣　王小明　饶刚　万秋红　方盛国. 从细胞色素b 基因全序列分析岩羊和山羊、绵羊的系统发生关系[J]. , 2004, 24(2): 109-114.
[12]	梁云媚, 王小明. 贺兰山岩羊的生命表和春夏季节社群结构的研究[J]. , 2000, 20(4): 258-262.
[13]	王小明,李明,　唐绍祥,　刘志霄. 春季岩羊种群生态学特征的初步研究[J]. , 1998, 18(1): 27-33.
[14]	任军让, 余玉群. 青海省玉树、果洛州岩羊的种群结构及生命表初探[J]. , 1990, 10(3): 189-193.
[15]	吴毅, 袁重桂, 胡锦矗, 彭基泰, 陶沛林. 矮岩羊生物学的研究[J]. , 1990, 10(3): 185-188.

宁夏贺兰山岩羊小反刍兽疫疫情来源分析

Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain, Ningxia, China

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