兽类学报 ›› 2023, Vol. 43 ›› Issue (2): 171-181.DOI: 10.16829/j.slxb.150673
阚威1,4, 李雷斌2,5, 赵旭阳3, 杨林2, 孙雨1,2(), 蔡金山1()
收稿日期:
2022-03-30
接受日期:
2022-11-10
出版日期:
2023-03-30
发布日期:
2023-03-23
通讯作者:
孙雨,蔡金山
作者简介:
阚威 (1986- ),男,兽医师,博士研究生,主要从事动物疫病防控工作.
基金资助:
Wei KAN1,4, Leibin LI2,5, Xuyang ZHAO3, Lin YANG2, Yu SUN1,2(), Jinshan CAI1()
Received:
2022-03-30
Accepted:
2022-11-10
Online:
2023-03-30
Published:
2023-03-23
Contact:
Yu SUN,Jinshan CAI
摘要:
2021年1月19日,青海省海西州都兰县巴隆乡伊克高里村发生岩羊不明原因的死亡,表现为离群独处、卧地不起、体质虚弱、觅食困难、肛门周围黑色附着物等现象。通过临床症状、病理学解剖及实时荧光RT-PCR诊断,为小反刍兽疫病毒 (PPRV) 感染。采用RT-PCR技术从病死岩羊病料组织中扩增出了PPRV N、F基因的部分片段。采用MegAlig、NT1和MEGA6.0软件对岩羊PPRV株N、F基因序列进行了比对和分析,绘制系统进化树,结果显示:此次岩羊感染的PPRV株N、F基因片段与新疆株 (China/Xinjiang/2015/16) 序列片段的同源性分别为99.43%和99.73%。遗传进化分析,该病原属于基因Ⅳ系。在N、F基因核酸序列水平上,与国内新疆地区分离毒株亲缘关系最近,同在一个小的分支;与国外毒株相比,N基因与塞内加尔和尼日利亚分离毒株亲缘关系较远,F基因与国外分离毒株亲缘关系较远。综上所述,青海岩羊源PPRV株属于基因Ⅳ系,与当前我国流行的野毒株属于同一个谱系。
中图分类号:
阚威, 李雷斌, 赵旭阳, 杨林, 孙雨, 蔡金山. 青海岩羊源小反刍兽疫病毒N、F基因的遗传进化分析[J]. 兽类学报, 2023, 43(2): 171-181.
Wei KAN, Leibin LI, Xuyang ZHAO, Lin YANG, Yu SUN, Jinshan CAI. Diagnosis of peste des petits ruminants and genetic evolution analysis of N and F genes of Pseudois nayaur in Qinghai[J]. ACTA THERIOLOGICA SINICA, 2023, 43(2): 171-181.
基因名称 Gene name | 毒株名称 Stains name | 宿主 Host | GenBank 登录号 GenBank accession NO. | 片段大小 Fragment size (bp) | 分离地区 Separation area | 所属支系 Branch line |
---|---|---|---|---|---|---|
N | ICV89 | 山羊Goat | EU267273.1 | 15 948 | 科特迪瓦 Cote d’Ivoire | Ⅰ |
E32/1969 | 山羊Goat | KP789375.1 | 15 948 | 塞内加尔 Senegal | Ⅰ | |
75/1 | 山羊Goat | KY628761.1 | 15 948 | 尼日利亚 Nigeria | Ⅱ | |
Ethiopia 1994 | 山羊Goat | KJ867540.1 | 15 948 | 埃塞俄比亚 Ethiopia | Ⅲ | |
Oman 1983 | 山羊Goat | KJ867544.1 | 15 948 | 阿曼 Oman | Ⅲ | |
China/XJBZ/2015 | 北山羊Capraibex | KT633939.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
HBHA1710 | 山羊Goat | MG420999.1 | 687 | 中国河北 Hebei, China | Ⅳ | |
ChinaGD2014 | 山羊Goat | MF443352.1 | 15 954 | 中国广东 Guangdong, China | Ⅳ | |
ChinaAH2014 | 山羊Goat | MF443354.1 | 15 954 | 中国安徽 Anhui, China | Ⅳ | |
ChinaGS2014 | 绵羊Sheep | MF443351.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
ChinaHB2014 | 山羊Goat | MF443348.1 | 15 954 | 中国河北 Hebei, China | Ⅳ | |
ChinaCQ2014 | 山羊Goat | MF443353.1 | 15 954 | 中国重庆 Chongqing, China | Ⅳ | |
ChinaGZ2014 | 山羊Goat | MF443349.1 | 15 954 | 中国贵州 Guizhou, China | Ⅳ | |
CH/HNZM/2014 | 山羊Goat | KM089832.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
CH/HNNY/2014 | 山羊Goat | KM089830.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
ChinaGS2018 | 普氏原羚 Procapra przewalskii | MN121838.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
Xinjiang2015-16 | 未知Unknown | KY434265.1 | 351 | 中国新疆 Xinjiang, China | Ⅳ | |
Xinjiang2015-59 | 未知Unknown | KY434302.1 | 351 | 中国新疆 Xinjiang, China | Ⅳ | |
China/XJYL/2013 | 山羊Goat | KM091959.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
F | ICV89 | 山羊Goat | EU267273.1 | 15 948 | 中国新疆 Xinjiang, China | Ⅰ |
E32/1969 | 山羊Goat | AJT59440.1 | 15 948 | 塞内加尔 Senegal | Ⅰ | |
Nigeria/75/1 | 山羊Goat | HQ197753.1 | 15 948 | 尼日利亚 Nigeria | Ⅱ | |
Nigeria/76/1 | 山羊Goat | EU267274.1 | 15 948 | 尼日利亚 Nigeria | Ⅱ | |
KJ867543.1 | 山羊Goat | KJ867543.1 | 15 948 | 乌干达 Uganda | Ⅲ | |
Ethiopia | 山羊Goat | KJ867540.1 | 15 948 | 埃塞俄比亚 Ethiopia | Ⅲ | |
Xinjiang2015-16 | 未知Unknown | KY434225.1 | 372 | 中国新疆 Xinjiang, China | Ⅳ | |
China/XJBZ/2015 | 北山羊Ibex | KT633939.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
ChinaGS2018 | 普氏原羚 Procapra przewalskii | MN121838.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
ChinaHeN2014 | 山羊Goat | MF443347.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
ChinaHLJ2014 | 山羊Goat | MF443346.1 | 15 954 | 中国黑龙江 Helongjiang, China | Ⅳ | |
China/XJYL/2013 | 山羊Goat | KM091959.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
ChinaHB2014 | 山羊Goat | MF443348.1 | 15 954 | 中国河北 Hebei, China | Ⅳ | |
CH/HNNY/2014 | 山羊Goat | KM089830.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
CH/HNZM/2014 | 山羊Goat | KM089832.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
ChinaGZ2014 | 山羊Goat | MF443349.1 | 15 954 | 中国贵州 Guizhou, China | Ⅳ | |
ChinaGD2014 | 山羊Goat | MF443352.1 | 15 954 | 中国广东 Guangdong, China | Ⅳ | |
ChinaAH2014 | 山羊Goat | MF443354.1 | 15 954 | 中国安徽 Anhui, China | Ⅳ | |
ChinaGS2014 | 绵羊Sheep | MF443351.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
ChinaGX2014 | 山羊Goat | MF443350.1 | 15 954 | 中国广西 Guangxi, China | Ⅳ |
表1 参考毒株信息
Table 1 Refer to virus strain information
基因名称 Gene name | 毒株名称 Stains name | 宿主 Host | GenBank 登录号 GenBank accession NO. | 片段大小 Fragment size (bp) | 分离地区 Separation area | 所属支系 Branch line |
---|---|---|---|---|---|---|
N | ICV89 | 山羊Goat | EU267273.1 | 15 948 | 科特迪瓦 Cote d’Ivoire | Ⅰ |
E32/1969 | 山羊Goat | KP789375.1 | 15 948 | 塞内加尔 Senegal | Ⅰ | |
75/1 | 山羊Goat | KY628761.1 | 15 948 | 尼日利亚 Nigeria | Ⅱ | |
Ethiopia 1994 | 山羊Goat | KJ867540.1 | 15 948 | 埃塞俄比亚 Ethiopia | Ⅲ | |
Oman 1983 | 山羊Goat | KJ867544.1 | 15 948 | 阿曼 Oman | Ⅲ | |
China/XJBZ/2015 | 北山羊Capraibex | KT633939.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
HBHA1710 | 山羊Goat | MG420999.1 | 687 | 中国河北 Hebei, China | Ⅳ | |
ChinaGD2014 | 山羊Goat | MF443352.1 | 15 954 | 中国广东 Guangdong, China | Ⅳ | |
ChinaAH2014 | 山羊Goat | MF443354.1 | 15 954 | 中国安徽 Anhui, China | Ⅳ | |
ChinaGS2014 | 绵羊Sheep | MF443351.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
ChinaHB2014 | 山羊Goat | MF443348.1 | 15 954 | 中国河北 Hebei, China | Ⅳ | |
ChinaCQ2014 | 山羊Goat | MF443353.1 | 15 954 | 中国重庆 Chongqing, China | Ⅳ | |
ChinaGZ2014 | 山羊Goat | MF443349.1 | 15 954 | 中国贵州 Guizhou, China | Ⅳ | |
CH/HNZM/2014 | 山羊Goat | KM089832.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
CH/HNNY/2014 | 山羊Goat | KM089830.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
ChinaGS2018 | 普氏原羚 Procapra przewalskii | MN121838.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
Xinjiang2015-16 | 未知Unknown | KY434265.1 | 351 | 中国新疆 Xinjiang, China | Ⅳ | |
Xinjiang2015-59 | 未知Unknown | KY434302.1 | 351 | 中国新疆 Xinjiang, China | Ⅳ | |
China/XJYL/2013 | 山羊Goat | KM091959.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
F | ICV89 | 山羊Goat | EU267273.1 | 15 948 | 中国新疆 Xinjiang, China | Ⅰ |
E32/1969 | 山羊Goat | AJT59440.1 | 15 948 | 塞内加尔 Senegal | Ⅰ | |
Nigeria/75/1 | 山羊Goat | HQ197753.1 | 15 948 | 尼日利亚 Nigeria | Ⅱ | |
Nigeria/76/1 | 山羊Goat | EU267274.1 | 15 948 | 尼日利亚 Nigeria | Ⅱ | |
KJ867543.1 | 山羊Goat | KJ867543.1 | 15 948 | 乌干达 Uganda | Ⅲ | |
Ethiopia | 山羊Goat | KJ867540.1 | 15 948 | 埃塞俄比亚 Ethiopia | Ⅲ | |
Xinjiang2015-16 | 未知Unknown | KY434225.1 | 372 | 中国新疆 Xinjiang, China | Ⅳ | |
China/XJBZ/2015 | 北山羊Ibex | KT633939.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
ChinaGS2018 | 普氏原羚 Procapra przewalskii | MN121838.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
ChinaHeN2014 | 山羊Goat | MF443347.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
ChinaHLJ2014 | 山羊Goat | MF443346.1 | 15 954 | 中国黑龙江 Helongjiang, China | Ⅳ | |
China/XJYL/2013 | 山羊Goat | KM091959.1 | 15 954 | 中国新疆 Xinjiang, China | Ⅳ | |
ChinaHB2014 | 山羊Goat | MF443348.1 | 15 954 | 中国河北 Hebei, China | Ⅳ | |
CH/HNNY/2014 | 山羊Goat | KM089830.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
CH/HNZM/2014 | 山羊Goat | KM089832.1 | 15 954 | 中国河南 Henan, China | Ⅳ | |
ChinaGZ2014 | 山羊Goat | MF443349.1 | 15 954 | 中国贵州 Guizhou, China | Ⅳ | |
ChinaGD2014 | 山羊Goat | MF443352.1 | 15 954 | 中国广东 Guangdong, China | Ⅳ | |
ChinaAH2014 | 山羊Goat | MF443354.1 | 15 954 | 中国安徽 Anhui, China | Ⅳ | |
ChinaGS2014 | 绵羊Sheep | MF443351.1 | 15 954 | 中国甘肃 Gansu, China | Ⅳ | |
ChinaGX2014 | 山羊Goat | MF443350.1 | 15 954 | 中国广西 Guangxi, China | Ⅳ |
图1 死亡岩羊病理学解剖结果. a:肺表面坏死、出血和充血肿大;b:肠道出血,肠系膜淋巴结肿大;c:心脏外膜出血;d:气管内壁条状出血
Fig. 1 Pathologic autopsy results of dead Pseudois nayaur. a: Necrosis,hemorrhage and hyperemia on the surface of the lungs; b: Intestinal bleeding, mesenteric lymph node enlargement; c: Heart outer membrane hemorrhage; d: Streaked bleeding on the inner wall of trachea
图2 岩羊PPRV株N、F基因扩增结果. a:N基因;b:F基因;M:DL5000 DNA Marker;1:阴性对照;2:阳性对照;3:肺;4:脾脏;5:小肠;6:肠系膜淋巴结
Fig.2 Pseudois nayaur PPRV strain N, F gene amplification results. a: N gene; b: F gene; M: DL5000 DNA Marker; 1: Negative control; 2: Positive control; 3: Plungs; 4: Spleen; 5: Small intestine; 6: Mesenteric lymph nodes
图3 岩羊PPRV株与参考毒株N基因序列比对结果.方框表示突变位点
Fig. 3 The results of N gene sequence alignmen between Pseudois nayaur PPRV strain and reference strains.Boxes indicate mutation sites
图4 岩羊PPRV株与参考毒株F基因序列比对结果.方框表示突变位点或缺失位点
Fig. 4 The results of F gene sequence alignmen between Pseudois nayaur PPRV strain and reference strains.Boxes indicate mutation sites or deletion sites
PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KY434265.1_PPRV_strain_Xinjiang_2015-16 | 99.43 | ||||||||||||||||
KY434302.1_PPRV_strain_Xinjiang_2015-59 | 99.14 | 99.71 | |||||||||||||||
KM089830.1_PPRV_isolate_CH/HNNY/2014 | 98.85 | 99.43 | 98.85 | ||||||||||||||
MN121838.1_PPRV_isolate_ChinaGS2018 | 98.85 | 99.43 | 98.85 | 99.33 | |||||||||||||
MF443354.1_PPRV_isolate_ChinaAH2014 | 98.85 | 99.43 | 98.85 | 99.74 | 99.52 | ||||||||||||
MF443353.1_PPRV_isolate_ChinaCQ2014 | 98.85 | 99.43 | 98.85 | 99.79 | 99.50 | 99.91 | |||||||||||
MF443352.1_PPRV_isolate_ChinaGD2014 | 98.85 | 99.43 | 98.85 | 99.70 | 99.49 | 99.92 | 99.87 | ||||||||||
MF443349.1_PPRV_isolate_ChinaGZ2014 | 98.85 | 99.43 | 98.85 | 99.79 | 99.51 | 99.91 | 99.96 | 99.87 | |||||||||
MF443351.1_PPRV_isolate_ChinaGS2014 | 98.85 | 99.43 | 98.85 | 99.75 | 99.54 | 99.97 | 99.92 | 99.94 | 99.92 | ||||||||
MF443348.1_PPRV_isolate_ChinaHB2014 | 98.85 | 99.43 | 98.85 | 99.75 | 99.50 | 99.91 | 99.92 | 99.87 | 99.92 | 99.92 | |||||||
MG420999.1_PPRV_isolate_HBHA1710 | 98.85 | 99.43 | 98.85 | 99.56 | 99.71 | 99.71 | 99.71 | 99.71 | 99.71 | 99.71 | 99.71 | ||||||
KT633939.1_PPRV_isolate_China/XJBZ/2015 | 98.85 | 99.43 | 98.85 | 99.19 | 98.97 | 99.39 | 99.36 | 99.36 | 99.36 | 99.42 | 99.34 | 99.71 | |||||
KM091959.1_PPRV_isolate_China/XJYL/2013 | 98.85 | 99.43 | 98.85 | 99.76 | 99.51 | 99.92 | 99.93 | 99.89 | 99.93 | 99.94 | 99.92 | 99.71 | 99.38 | ||||
KM089832.1_PPRV_isolate_CH/HNZM/2014 | 98.85 | 99.43 | 98.85 | 99.65 | 99.36 | 99.77 | 99.82 | 99.74 | 99.82 | 99.79 | 99.79 | 99.71 | 99.22 | 99.79 | |||
EU267273.1_PPRV_strain_ICV89 | 83.08 | 83.79 | 83.12 | 87.65 | 87.62 | 87.84 | 87.84 | 87.83 | 87.87 | 87.86 | 87.83 | 88.33 | 87.96 | 87.87 | 87.72 | ||
KP789375.1_PPRV_isolate_E32/1969 | 85.63 | 86.32 | 85.67 | 89.26 | 89.21 | 89.45 | 89.44 | 89.44 | 89.47 | 89.47 | 89.44 | 90.40 | 89.57 | 89.47 | 89.31 | 97.07 | |
KY628761.1_PPRV_strain_75/1 | 87.71 | 88.39 | 87.74 | 91.52 | 91.44 | 91.73 | 91.72 | 91.69 | 91.72 | 91.73 | 91.72 | 91.61 | 92.12 | 91.72 | 91.58 | 90.28 | 91.99 |
KJ867544.1_PPRV_isolate_Oman_1983 | 80.02 | 80.02 | 79.31 | 86.47 | 86.54 | 86.69 | 86.68 | 86.65 | 86.70 | 86.71 | 86.68 | 85.12 | 86.78 | 86.69 | 86.54 | 87.97 | 89.60 |
KJ867540.1_PPRV_isolate_Ethiopia_1994 | 83.29 | 83.29 | 82.61 | 86.64 | 86.73 | 86.87 | 86.86 | 86.83 | 86.88 | 86.88 | 86.86 | 87.58 | 86.95 | 86.86 | 86.73 | 88.03 | 89.58 |
表2 岩羊PPRV株与参考毒株N基因同源性分析结果
Table 2 The results of homology analysis Pseudois nayaur PPRV strain N gene and reference strains
PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | PPRV N | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KY434265.1_PPRV_strain_Xinjiang_2015-16 | 99.43 | ||||||||||||||||
KY434302.1_PPRV_strain_Xinjiang_2015-59 | 99.14 | 99.71 | |||||||||||||||
KM089830.1_PPRV_isolate_CH/HNNY/2014 | 98.85 | 99.43 | 98.85 | ||||||||||||||
MN121838.1_PPRV_isolate_ChinaGS2018 | 98.85 | 99.43 | 98.85 | 99.33 | |||||||||||||
MF443354.1_PPRV_isolate_ChinaAH2014 | 98.85 | 99.43 | 98.85 | 99.74 | 99.52 | ||||||||||||
MF443353.1_PPRV_isolate_ChinaCQ2014 | 98.85 | 99.43 | 98.85 | 99.79 | 99.50 | 99.91 | |||||||||||
MF443352.1_PPRV_isolate_ChinaGD2014 | 98.85 | 99.43 | 98.85 | 99.70 | 99.49 | 99.92 | 99.87 | ||||||||||
MF443349.1_PPRV_isolate_ChinaGZ2014 | 98.85 | 99.43 | 98.85 | 99.79 | 99.51 | 99.91 | 99.96 | 99.87 | |||||||||
MF443351.1_PPRV_isolate_ChinaGS2014 | 98.85 | 99.43 | 98.85 | 99.75 | 99.54 | 99.97 | 99.92 | 99.94 | 99.92 | ||||||||
MF443348.1_PPRV_isolate_ChinaHB2014 | 98.85 | 99.43 | 98.85 | 99.75 | 99.50 | 99.91 | 99.92 | 99.87 | 99.92 | 99.92 | |||||||
MG420999.1_PPRV_isolate_HBHA1710 | 98.85 | 99.43 | 98.85 | 99.56 | 99.71 | 99.71 | 99.71 | 99.71 | 99.71 | 99.71 | 99.71 | ||||||
KT633939.1_PPRV_isolate_China/XJBZ/2015 | 98.85 | 99.43 | 98.85 | 99.19 | 98.97 | 99.39 | 99.36 | 99.36 | 99.36 | 99.42 | 99.34 | 99.71 | |||||
KM091959.1_PPRV_isolate_China/XJYL/2013 | 98.85 | 99.43 | 98.85 | 99.76 | 99.51 | 99.92 | 99.93 | 99.89 | 99.93 | 99.94 | 99.92 | 99.71 | 99.38 | ||||
KM089832.1_PPRV_isolate_CH/HNZM/2014 | 98.85 | 99.43 | 98.85 | 99.65 | 99.36 | 99.77 | 99.82 | 99.74 | 99.82 | 99.79 | 99.79 | 99.71 | 99.22 | 99.79 | |||
EU267273.1_PPRV_strain_ICV89 | 83.08 | 83.79 | 83.12 | 87.65 | 87.62 | 87.84 | 87.84 | 87.83 | 87.87 | 87.86 | 87.83 | 88.33 | 87.96 | 87.87 | 87.72 | ||
KP789375.1_PPRV_isolate_E32/1969 | 85.63 | 86.32 | 85.67 | 89.26 | 89.21 | 89.45 | 89.44 | 89.44 | 89.47 | 89.47 | 89.44 | 90.40 | 89.57 | 89.47 | 89.31 | 97.07 | |
KY628761.1_PPRV_strain_75/1 | 87.71 | 88.39 | 87.74 | 91.52 | 91.44 | 91.73 | 91.72 | 91.69 | 91.72 | 91.73 | 91.72 | 91.61 | 92.12 | 91.72 | 91.58 | 90.28 | 91.99 |
KJ867544.1_PPRV_isolate_Oman_1983 | 80.02 | 80.02 | 79.31 | 86.47 | 86.54 | 86.69 | 86.68 | 86.65 | 86.70 | 86.71 | 86.68 | 85.12 | 86.78 | 86.69 | 86.54 | 87.97 | 89.60 |
KJ867540.1_PPRV_isolate_Ethiopia_1994 | 83.29 | 83.29 | 82.61 | 86.64 | 86.73 | 86.87 | 86.86 | 86.83 | 86.88 | 86.88 | 86.86 | 87.58 | 86.95 | 86.86 | 86.73 | 88.03 | 89.58 |
PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KY434225.1_PPRV_strain_Xinjiang_2015-16 | 99.73 | ||||||||||||||||||
MN121838.1_PPRV_isolate_ChinaGS2018 | 98.64 | 98.92 | |||||||||||||||||
MF443354.1_PPRV_isolate_ChinaAH2014 | 98.64 | 98.92 | 99.52 | ||||||||||||||||
MF443352.1_PPRV_isolate_ChinaGD2014 | 98.64 | 98.92 | 99.49 | 99.92 | |||||||||||||||
MF443351.1_PPRV_isolate_ChinaGS2014 | 98.64 | 98.92 | 99.54 | 99.97 | 99.94 | ||||||||||||||
MF443350.1_PPRV_isolate_ChinaGX2014 | 98.64 | 98.92 | 99.52 | 99.95 | 99.92 | 99.97 | |||||||||||||
MF443349.1_PPRV_isolate_ChinaGZ2014 | 98.64 | 98.92 | 99.51 | 99.91 | 99.87 | 99.92 | 99.91 | ||||||||||||
MF443348.1_PPRV_isolate_ChinaHB2014 | 98.64 | 98.92 | 99.50 | 99.91 | 99.87 | 99.92 | 99.91 | 99.92 | |||||||||||
MF443347.1_PPRV_isolate_ChinaHeN2014 | 98.64 | 98.92 | 99.55 | 99.92 | 99.89 | 99.94 | 99.92 | 99.89 | 99.90 | ||||||||||
MF443346.1_PPRV_isolate_ChinaHLJ2014 | 98.64 | 98.92 | 99.55 | 99.92 | 99.89 | 99.94 | 99.92 | 99.89 | 99.90 | 99.95 | |||||||||
KT633939.1_PPRV_isolate_China/XJBZ/2015(4) | 98.36 | 98.64 | 98.97 | 99.39 | 99.36 | 99.42 | 99.39 | 99.36 | 99.34 | 99.36 | 99.36 | ||||||||
KM091959.1_PPRV_isolate_China/XJYL/2013(4) | 98.64 | 98.92 | 99.51 | 99.92 | 99.89 | 99.94 | 99.92 | 99.93 | 99.92 | 99.91 | 99.91 | 99.38 | |||||||
KM089832.1_PPRV_isolate_CH/HNZM/2014(4) | 98.08 | 98.37 | 99.36 | 99.77 | 99.74 | 99.79 | 99.77 | 99.82 | 99.79 | 99.75 | 99.75 | 99.22 | 99.79 | ||||||
KM089830.1_PPRV_isolate_CH/HNNY/2014(4) | 98.64 | 98.92 | 99.33 | 99.74 | 99.70 | 99.75 | 99.74 | 99.79 | 99.75 | 99.72 | 99.72 | 99.19 | 99.76 | 99.65 | |||||
EU267273.1_PPRV_virus_strain_ICV89(1) | 89.36 | 89.71 | 88.10 | 88.24 | 88.32 | 88.32 | 88.32 | 88.32 | 88.24 | 88.32 | 88.32 | 88.76 | 88.32 | 88.02 | 88.17 | ||||
AJT59440.1_PPRV_isolate_E32/1969(1) | 89.65 | 90.00 | 90.28 | 90.42 | 90.49 | 90.49 | 90.49 | 90.49 | 90.42 | 90.49 | 90.49 | 90.91 | 90.49 | 90.21 | 90.35 | 97.25 | |||
HQ197753.1_PPRV_strain_Nigeria/75/1(2) | 93.17 | 93.48 | 92.13 | 92.40 | 92.34 | 92.34 | 92.34 | 92.34 | 92.27 | 92.34 | 92.34 | 93.56 | 92.34 | 92.06 | 92.20 | 90.61 | 92.89 | ||
EU267274.1_PPRV_strain_Nigeria/76/1(2) | 94.04 | 94.35 | 92.90 | 93.17 | 93.10 | 93.10 | 93.10 | 93.10 | 93.03 | 93.10 | 93.10 | 93.91 | 93.10 | 92.83 | 92.96 | 91.67 | 93.71 | 97.89 | |
KJ867543.1_PPRV_isolate_Uganda_2012(3) | 89.05 | 89.40 | 87.99 | 87.99 | 88.06 | 88.06 | 88.06 | 88.06 | 87.98 | 88.06 | 88.06 | 88.20 | 88.06 | 87.91 | 87.91 | 90.03 | 91.70 | 91.06 | 92.04 |
KJ867540.1_PPRV_isolate_Ethiopia_1994(3) | 86.70 | 87.07 | 86.53 | 86.53 | 86.61 | 86.61 | 86.61 | 86.61 | 86.53 | 86.61 | 86.61 | 86.61 | 86.61 | 86.46 | 86.46 | 88.31 | 90.41 | 88.52 | 89.69 |
表3 岩羊PPRV株与参考毒株F基因同源性分析结果
Table 3 The results of homology analysis Pseudois nayaur PPRV strain F gene and reference strains
PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | PPRV F | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KY434225.1_PPRV_strain_Xinjiang_2015-16 | 99.73 | ||||||||||||||||||
MN121838.1_PPRV_isolate_ChinaGS2018 | 98.64 | 98.92 | |||||||||||||||||
MF443354.1_PPRV_isolate_ChinaAH2014 | 98.64 | 98.92 | 99.52 | ||||||||||||||||
MF443352.1_PPRV_isolate_ChinaGD2014 | 98.64 | 98.92 | 99.49 | 99.92 | |||||||||||||||
MF443351.1_PPRV_isolate_ChinaGS2014 | 98.64 | 98.92 | 99.54 | 99.97 | 99.94 | ||||||||||||||
MF443350.1_PPRV_isolate_ChinaGX2014 | 98.64 | 98.92 | 99.52 | 99.95 | 99.92 | 99.97 | |||||||||||||
MF443349.1_PPRV_isolate_ChinaGZ2014 | 98.64 | 98.92 | 99.51 | 99.91 | 99.87 | 99.92 | 99.91 | ||||||||||||
MF443348.1_PPRV_isolate_ChinaHB2014 | 98.64 | 98.92 | 99.50 | 99.91 | 99.87 | 99.92 | 99.91 | 99.92 | |||||||||||
MF443347.1_PPRV_isolate_ChinaHeN2014 | 98.64 | 98.92 | 99.55 | 99.92 | 99.89 | 99.94 | 99.92 | 99.89 | 99.90 | ||||||||||
MF443346.1_PPRV_isolate_ChinaHLJ2014 | 98.64 | 98.92 | 99.55 | 99.92 | 99.89 | 99.94 | 99.92 | 99.89 | 99.90 | 99.95 | |||||||||
KT633939.1_PPRV_isolate_China/XJBZ/2015(4) | 98.36 | 98.64 | 98.97 | 99.39 | 99.36 | 99.42 | 99.39 | 99.36 | 99.34 | 99.36 | 99.36 | ||||||||
KM091959.1_PPRV_isolate_China/XJYL/2013(4) | 98.64 | 98.92 | 99.51 | 99.92 | 99.89 | 99.94 | 99.92 | 99.93 | 99.92 | 99.91 | 99.91 | 99.38 | |||||||
KM089832.1_PPRV_isolate_CH/HNZM/2014(4) | 98.08 | 98.37 | 99.36 | 99.77 | 99.74 | 99.79 | 99.77 | 99.82 | 99.79 | 99.75 | 99.75 | 99.22 | 99.79 | ||||||
KM089830.1_PPRV_isolate_CH/HNNY/2014(4) | 98.64 | 98.92 | 99.33 | 99.74 | 99.70 | 99.75 | 99.74 | 99.79 | 99.75 | 99.72 | 99.72 | 99.19 | 99.76 | 99.65 | |||||
EU267273.1_PPRV_virus_strain_ICV89(1) | 89.36 | 89.71 | 88.10 | 88.24 | 88.32 | 88.32 | 88.32 | 88.32 | 88.24 | 88.32 | 88.32 | 88.76 | 88.32 | 88.02 | 88.17 | ||||
AJT59440.1_PPRV_isolate_E32/1969(1) | 89.65 | 90.00 | 90.28 | 90.42 | 90.49 | 90.49 | 90.49 | 90.49 | 90.42 | 90.49 | 90.49 | 90.91 | 90.49 | 90.21 | 90.35 | 97.25 | |||
HQ197753.1_PPRV_strain_Nigeria/75/1(2) | 93.17 | 93.48 | 92.13 | 92.40 | 92.34 | 92.34 | 92.34 | 92.34 | 92.27 | 92.34 | 92.34 | 93.56 | 92.34 | 92.06 | 92.20 | 90.61 | 92.89 | ||
EU267274.1_PPRV_strain_Nigeria/76/1(2) | 94.04 | 94.35 | 92.90 | 93.17 | 93.10 | 93.10 | 93.10 | 93.10 | 93.03 | 93.10 | 93.10 | 93.91 | 93.10 | 92.83 | 92.96 | 91.67 | 93.71 | 97.89 | |
KJ867543.1_PPRV_isolate_Uganda_2012(3) | 89.05 | 89.40 | 87.99 | 87.99 | 88.06 | 88.06 | 88.06 | 88.06 | 87.98 | 88.06 | 88.06 | 88.20 | 88.06 | 87.91 | 87.91 | 90.03 | 91.70 | 91.06 | 92.04 |
KJ867540.1_PPRV_isolate_Ethiopia_1994(3) | 86.70 | 87.07 | 86.53 | 86.53 | 86.61 | 86.61 | 86.61 | 86.61 | 86.53 | 86.61 | 86.61 | 86.61 | 86.61 | 86.46 | 86.46 | 88.31 | 90.41 | 88.52 | 89.69 |
图5 岩羊PPRV株与参考毒株N基因系统进化分析结果. N为岩羊PPRV株N基因;系统发育树各支系数字代表自展值;Ⅰ、Ⅱ、Ⅲ、Ⅳ表示PPRV的4个基因系,Ⅰ系和Ⅱ系主要分布于西非,Ⅲ系分布于东非、阿拉伯及印度南部,Ⅳ系分布于中东、阿拉伯和印度次大陆
Fig. 5 Phylogenetic analysis of N gene of Pseudois nayaur PPRV strain and reference strains. N is the Pseudois nayaur PPRV N gene;The numbers of each branch of the phylogenetic tree represent the self-expanding value; Ⅰ, Ⅱ, Ⅲ, Ⅳ. represent the four gene lines of PPRV, Ⅰ and Ⅱ are mainly distributed in West Africa, III are distributed in East Africa, Arabia and southern India, and IV.are distributed in the Middle East, Arabia and the Indian subcontinent
图6 岩羊PPRV株与参考毒株F基因基因系统进化分析结果.F为岩羊PPRV株 F基因;KY434225.1为GenBank PPRV F 参考基因序列号系统发育树各支系数字代表自展值;Ⅰ、Ⅱ、Ⅲ、Ⅳ表示PPRV的4个基因系,Ⅰ系和Ⅱ系主要分布于西非,Ⅲ系分布于东非、阿拉伯及印度南部,Ⅳ系分布于中东、阿拉伯和印度次大陆
Fig. 6 Phylogenetic analysis of F gene of Pseudois nayaur PPRV strain and reference strains. F is the Pseudois nayaur PPRV F gene; KY434225.1 is the reference sequence number of GenBank PPRV F gene. The numbers of each branch of the phylogenetic tree represent the self-expanding value; Ⅰ, Ⅱ, Ⅲ, Ⅳ. represent the four gene lines of PPRV, Ⅰ and Ⅱ are mainly distributed in West Africa, Ⅲ are distributed in East Africa, Arabia and southern India, and Ⅳ are distributed in the Middle East, Arabia and the Indian subcontinent
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