Analysis of the chemical components in the urine of the tree shrew (Tupaia belangeri) by HS-SPME-GC/MS

doi:10.16829/j.slxb.150077

ACTA THERIOLOGICA SINICA ›› 2018, Vol. 38 ›› Issue (1): 85-94.DOI: 10.16829/j.slxb.150077

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Analysis of the chemical components in the urine of the tree shrew (Tupaia belangeri) by HS-SPME-GC/MS

YE Fangyan, ZUO Mulin, ZHU Wanlong, GAO Wenrong, ZHANG Hao, HOU Dongmin, WANG Zhengkun

Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of University in Yunnan Province, School of Life Science, Yunnan Normal University

Online:2018-01-30 Published:2018-04-09

HS-SPME-GC/MS法分析中缅树鼩尿液的化学成分

叶芳艳左木林朱万龙高文荣张浩侯东敏王政昆

云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室，云南师范大学生命科学学院

通讯作者: 王政昆 E-mail：wangzk_123@163.com
基金资助:
国家国际科技合作专项项目 (2014DFR31040)；国家自然科学基金项目 (31660121)；云南省科技计划项目 (2015GA008)云南省应用基础研究计划重点项目 (2016FA045)

Abstract

Abstract: Animal urine plays a very important role in animal life. The detection of chemical compounds in Tupaia belangeri urine is the key to identify its composition and assay the function of each chemical compound. In the present study, headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were used to analyze the urine compositions in Tupaia belangeri. Through searching the mass spectrometry library of the National Institute of Standards and Technology (NIST), we found that the chemical compounds belong mainly to aldehydes, ketones, acids, esters, aromatic hydrocarbons, hydrocarbons, and phenols, etc. The results showed that HS-SPME-GC/MS is an effective method to detect volatile and semi-volatile compounds in tree shrew urine. In addition, wild individuals with relatively abundant food sources have more components than do those fed on an artificial diet., There are sex differences in the chemical compositions of urine. The results provide a foundation for studying the mechanism of chemical communication in tree shrews.

Key words: Chemical communication, Chemical composition, Tupaia belangeri, Urine

关键词: 中缅树鼩, 尿液, 化学通讯, 化学成分

YE Fangyan, ZUO Mulin, ZHU Wanlong, GAO Wenrong, ZHANG Hao, HOU Dongmin, WANG Zhengkun. Analysis of the chemical components in the urine of the tree shrew (Tupaia belangeri) by HS-SPME-GC/MS[J]. ACTA THERIOLOGICA SINICA, 2018, 38(1): 85-94.

叶芳艳左木林朱万龙高文荣张浩侯东敏王政昆. HS-SPME-GC/MS法分析中缅树鼩尿液的化学成分[J]. 兽类学报, 2018, 38(1): 85-94.

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Analysis of the chemical components in the urine of the tree shrew (Tupaia belangeri) by HS-SPME-GC/MS

HS-SPME-GC/MS法分析中缅树鼩尿液的化学成分

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