Abstract: The insulin-like growth factor Ⅰ ( IGF-Ⅰ) cDNA of the golden monkey ( Rhinopithecus roxellana) was cloned for the first time from liver tissue by reverse transcription and amplified by PCR using a set of primers that we designed in this study. The amplified cDNA fragment was inserted into the vector pGEM-T. Through transferring, screening, enzymolysis and sequencing, it was demonstrated that this fragment was IGF-Ⅰgene cDNA. It is 521nts in length and contains one open reading frame (ORF), which encodes for a polypeptide containing 153 amino acids. The golden monkey IGF-Ⅰ gene has a high homology with othermammal IGF-Ⅰgenes.

Key words: Cloning, Golden monkey, IGF-Ⅰgene, Sequence analysis

摘要： 本实验从金丝猴肝脏细胞RNA 中反转录得到cDNA , 利用设计的引物M1 和M2 扩增IGF-Ⅰ基因, 并将其克隆到pGEM- T载体上。经筛选、酶切、PCR 鉴定、序列分析, 证明该片段为金丝猴IGF- Ⅰ基因的cDNA 克隆。该片段由521 个核苷酸组成, 其中包括一个完整的开读框(ORF) , 编码一个由153 个氨基酸组成的多肽。与GenBank 中人、猪、鼠、马、牛、兔、山羊等哺乳动物的IGF-Ⅰ序列比较, 其编码成熟肽序列的同源性从93. 8%(马) 到88.5% (鼠) , 开放框序列的同源性从94.4 % (人) 到88.5% (鼠) 。

关键词: 金丝猴, IGF-Ⅰ基因, 克隆, 序列分析