Abstract: Phytohaemagglutinin (PHA)induced swelling response is an immunological parameter,which is widely used in the field of ecological immunology The swelling area of PHA post-injection is roughly equal to the strengths of the cell- mediated immune response and innate immunity as a comprehensive variation. To explore the response patterns of PHA-in-duced swelling during reproduction in small mammals,we measured the dynamic variations of skin tissue thickness at 0 h pre-injection,as well as 6 h,12 h and 24 h after PHA versus PBS injection at different reproductive phases (non-productive, pregnant, lactation,and post lactation phase)in striped hamster,Cricetulus barabensis. The results were as follows:(1)Hamsters showed two types of PHA response at different productive phases. One type response peaked 6 h after PHA injection for the non-reproductive,pregnant,and post lactation phases,then gradually declined at 12 h and 24 h. The other response did not show significant differences among different time points for the lactation phase. (2)Maximum PHA response for the lactation phase showed a reduced tendency,but no significant differences were observed among different reproductive phases. Our results indicate the PHA response pattern for the lactation phase is different from that of other reproductive phases,and maximum PHA response is delayed,which may be harmful to maternal survival in a short term,while it is beneficial to promote reproductive process and accelerate offspring’s growth and development.

Key words: Striped hamster(Cricetulus barabensis), Lactation, Phytohemagglutinin, PHA, Reproduction

摘要： 由植物血凝素（Phytohemagglutinin，ＰＨＡ） 诱导产生的肿胀反应是生态免疫学中被广泛使用的免疫学参数，通过注射部位的肿胀程度大体上可反映细胞介导的免疫和天然免疫的综合变化情况。为探讨小型哺乳动物不同繁殖状态对ＰＨＡ 的反应模式，以非繁殖期、妊娠期、哺乳期和断乳期的雌性黑线仓鼠为研究对象，测定了注射ＰＨＡ 和生理盐水前（０ ｈ）及注射后６ ｈ、１２ ｈ 和２４ ｈ 注射部位皮肤组织的增厚程度。结果显示：（１）黑线仓鼠对ＰＨＡ 的反应有两种模式：非繁殖期、妊娠期和断乳期对ＰＨＡ 的反应模式相似，都在注射后６ ｈ 最高，１２ ｈ 和２４ ｈ 后逐渐下降；哺乳期对ＰＨＡ 的反应在注射后６ ｈ、１２ ｈ 和２４ ｈ 接近，彼此间的差异均不显著。（２）哺乳期黑线仓鼠的ＰＨＡ 反应高峰值有下降趋势，但与非繁殖期、妊娠期和断乳期高峰值之间的差异不显著。结果表明，黑线仓鼠在哺乳期对ＰＨＡ 的反应模式不同于其他繁殖阶段，且反应的高峰值被延迟，这对其存活可能有害，但有助于繁殖进程的延续和后代的生长发育。

关键词: 黑线仓鼠, 植物血凝素, 哺乳期, 繁殖