ACTA THERIOLOGICA SINICA ›› 2022, Vol. 42 ›› Issue (4): 432-441.DOI: 10.16829/j.slxb.150637
• ORIGINAL PAPERS • Previous Articles Next Articles
Xiaoqi WANG1,2,3, Wenjing HAO1, Zhichao ZHANG1, Jing HAN1,4, Rujing WANG2,3, Ziyuan DUAN1()
Received:
2021-11-15
Accepted:
2022-05-06
Online:
2022-07-30
Published:
2022-07-22
Contact:
Ziyuan DUAN
王小琪1,2,3, 郝文静1, 张志超1, 韩晶1,4, 王儒敬2,3, 段子渊1()
通讯作者:
段子渊
作者简介:
王小琪 (1989- ),女,博士研究生,主要从事肠道微生物组研究.
基金资助:
CLC Number:
Xiaoqi WANG, Wenjing HAO, Zhichao ZHANG, Jing HAN, Rujing WANG, Ziyuan DUAN. Fecal microbiota diversity analysis of the diarrheal sub-adult Qaidam horses and biomarkers screening[J]. ACTA THERIOLOGICA SINICA, 2022, 42(4): 432-441.
王小琪, 郝文静, 张志超, 韩晶, 王儒敬, 段子渊. 腹泻柴达木马亚成体粪便微生物多样性分析及其生物标记物的筛选[J]. 兽类学报, 2022, 42(4): 432-441.
属Genera | 引物Primers (5'-3') | 参考文献References |
---|---|---|
拟杆菌属 Bacteroides | F: CTGAACCAGCCAAGTAGCG | |
R: CCGCAAACTTTCACAACTGACTTA | ||
普雷沃菌属 Prevotella | F: GGTTCTGAGAGGAAGGTCCCC | |
R: TCCTGCACGCTACTTGGCTG | ||
甲烷短杆菌属 Methanobrevibacter | F: CCTCCGCAATGTGAGAAATCGC | |
R: TCWCCAGCAATTCCCACCAGTT | ||
双歧杆菌属 Bifidobacterium | F: CGCGTCYGGTGTGAAAG | |
R: CCCCACATCCAGCATCCA |
Table 1 PCR amplification primers of bacterial 16S rRNA gene in Qaidam horses’ feces
属Genera | 引物Primers (5'-3') | 参考文献References |
---|---|---|
拟杆菌属 Bacteroides | F: CTGAACCAGCCAAGTAGCG | |
R: CCGCAAACTTTCACAACTGACTTA | ||
普雷沃菌属 Prevotella | F: GGTTCTGAGAGGAAGGTCCCC | |
R: TCCTGCACGCTACTTGGCTG | ||
甲烷短杆菌属 Methanobrevibacter | F: CCTCCGCAATGTGAGAAATCGC | |
R: TCWCCAGCAATTCCCACCAGTT | ||
双歧杆菌属 Bifidobacterium | F: CGCGTCYGGTGTGAAAG | |
R: CCCCACATCCAGCATCCA |
组别 Groups | 有效序列片段数 No. of filtered reads | 操作分类单元数 OTUs | 分类单元(属) Taxa (genus) | 样本覆盖度 Coverage |
---|---|---|---|---|
腹泻组 Diarrhea | 117 711 | 4 501 | 149 | 0.975 |
健康组 Naive control | 457 643 | 6 030 | 164 | 0.972 |
Table 2 16S rRNA sequencing data of feces from Qaidam horses
组别 Groups | 有效序列片段数 No. of filtered reads | 操作分类单元数 OTUs | 分类单元(属) Taxa (genus) | 样本覆盖度 Coverage |
---|---|---|---|---|
腹泻组 Diarrhea | 117 711 | 4 501 | 149 | 0.975 |
健康组 Naive control | 457 643 | 6 030 | 164 | 0.972 |
Fig. 1 The fecal microbial diversity of Qaidam horses. A: The Alpha diversity of fecal bacteria (mean ± SD); B: PCoA plot based on Unweighted Unifrac matrix.* P < 0.05
Fig. 2 Comparison of relative abundance of fecal microbial in diarrhea (Dia) group and naive control (NC) group of Qaidam horses at phylum level. A: The histograms of microbiota abundances at phylum level; B: The phyla with significant difference between two groups. * P < 0.05
Fig. 3 Comparison of relative abundance of fecal microbial in diarrhea (Dia) group and naive control (NC) group of Qaidam horses at genus level. A: The histograms of microbiota abundance at genus level; B: Clustering heatmap of genera with significant difference between two groups
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