Abstract: Doubly labeled water(DLW ) method is the most correct method for measuring enerofwildanimals,and themost difficult key methodis the quantitative analysis for isotopesof body water in animals.For testing reliability of isotope measurements indifferent laboratories,the Chengdu Collegeof：Geology (CCG)and the University of Colifornia at Los Angeles (UCLA) had cooperated on measuring identical blood samples taken from doubly labeled goats living near Chengdu in May of1990,and got a successful result.The deuterium values determind by each laboratory were neary identical,but the ¹⁸O results from UCLA wereconsistently higher than those from CCG.However,the energy metabolism values determined by each laboratory were very similar.Metabolic rate valurs differed between laboratories by 6.6% on average,and water influx rates differed by an average of 1.2%,but neither difference is statistically significant. Because the UCLA laboratory has been calibrated against laboratories in United Kingdom and in the Netherland,the determined results in the isotope laboratories in CCG and UCLA have been validated for DLW method. This paper tries to discuss the error source in the different laboratories,and has a reference significance for the similar laboratories.

Key words: Doubly labeled water(DLW), Isotope, Energy metabolism

摘要： 双标水（double labeled water,DLW）法是研究野外动物能量代谢最准确的方法，其中最关键、最困难的环节是同位素的定量分析。为了检验不同同位素实验室测定动物血样氢氧同位素丰富度的准确性及其估算对能量代谢的影响程度，成都地质学院（CCG）和美国加利福尼亚大学洛杉矶分校（UCLA）的同位素实验室于1990年5月开展了合作，以成都附近农村的5只山羊为研究对象进行了双标水的实验。将采集的动物血样分别在两个实验室进行了D和¹⁸O含量测定，取得了成功的效果：两个实验室所测得的¹⁸O值近于相同，而UCLA同位素实验室测得的¹⁸O值略高于CCG的测定值。由此计算出的能量代谢值却非常接近，其差值平均为6.6%;水循环平均相差1.2%，差异均不显著（P>0.05）。 UCLA实验室的质谱仪已为英国和荷兰的同类实验室所校正，这确认了我们的合作测定成果的可信度和有效性，证明CCG和UCLA实验室的测定结果均可以满足双标水法同位素定量测定的需要。测定结果是可信的。本文还就两地实验室所测结果产生误差的原因进行了初步分析，对于同类研究的其它实验室也有借鉴意义。