Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence

Abstract

Abstract: In order to identify an unknown whale vertebra's species,genomic DNA is extracted from the bone powder by proteinase K and phenol /chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR (Polymerase Chain Reaction). The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai, it is 99%. It indicates that the vertebra is Balaenoptera omurai. The phylogenetic tree based on UPGMA is consistent with the blasting result. Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1 -2 nucleotides which are different from the homologous sequence of B. omurai (accession AB201257,AB201256), thus, the sequence is a new haplotype This is the first record of B. omurai which distributes in the South China Sea.

Key words: Balaenoptera omurai, Cytochrome b gene, Phylogenetic tree, Species identification, Whale vertebra

关键词: 鲸类脊椎骨, 细胞色素b 基因, 分子进化树, 种类鉴定, 大村鲸

MA Mu,ZHU Qian, LI Xiang, SUN Yumiao, LIU Yingying, LIU Yanan, TAO Cuihua. Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence[J]. .

马牧, 祝茜, 李响, 孙玉苗, 刘莹莹, 刘亚楠, 陶翠花. 利用Cyt b 基因由未知鲸骨鉴定出大村鲸[J]. .

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[7]	Hung Sun Koh, Shar Seter, ZHANG Minghai, WANG Jinxing, Kyung Hee Jang, Davaa Bazarsad. Lack of mitochondrial DNA sequence divergence in two subspecies of the striped field mouse (Apodemus agrarius mantchuricus from Northeastern China and nearby Far-eastern Russia and A. a. coreae from the Korean Peninsula) [J]. , 2011, 31(1): 84-89.
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[9]	Hung Sun Koh,Kyung Hee Jang,WANG Jinxing,Bae Keun Lee,Nam Jung Park. Sequence diversity of the mitochondrial DNA cytochrome b gene and con- trol region in the two subspecies of the Korean field mouse (Apodemus peninsulae) [J]. , 2010, 30(3): 256-263.
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[12]	WANG Jiang,FANG Shengguo. Molecular Phylogenetic Relationships dactyla，Bovidae) Based on Sequence for Procapra in Antilopinae (Artiodactyia,Bovidae) Based on Sequence of Mitochondrial Cytochrome b Gene [J]. , 2005, 25(2): 105-114.
[13]	ZHANGWei XU Yanchun. A Review and Prospects of the Research on Hair Microstructure [J]. , 2003, 23(4): 339-345.
[14]	WANG Jialian　YANG Guang　LIU Hai 　ZHOU Kaiya 　WEI Fuwen. Application of Mitochondrial DNA Sequences in the Species Identif ication of Common Dolphins ( Genus Delphinus) in Chinese Waters [J]. , 2003, 23(2): 120-126.

Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence

利用Cyt b 基因由未知鲸骨鉴定出大村鲸

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