Effects of captivity on the assembly process of microbiota communities of plateau pikas
Received date: 2022-08-02
Accepted date: 2022-08-22
Online published: 2022-09-21
Revealing the community assembly process is helpful to better understand the mechanism of biodiversity generation and maintenance, which has been an emerging topic in microbial community ecology in recent years. Studies have shown that captivity can cause drastic changes in the composition and diversity of intestinal microbiotas, however, whether captivity can alter the assembly processes of gut bacterial communities in plateau pikas is still unknown. Here, we employed 16S rRNA gene-based large-scale structural profiling to explore the effect of captivity and breeding status on the gut microbial composition, diversity, functions, and assembly processes in plateau pikas (Ochotona curzoniae). The results showed that the community richness and community coverage index of captivity animals were significantly lower than those of wild groups. For the wild groups, the indexes were significantly higher in non-breeding groups than those of breeding groups. The relative abundance of Bacteroidetes was significantly higher in the captivity group than that of the wild groups, while the relative abundance of Firmicutes and Planctomycetes were significantly higher in wild groups than those of the captivity group. For the wild groups, the richness of Epsilonbacteraeota and Tenericutes were higher in the breeding period group than those of the non-breeding group. The results of functional prediction revealed that the abundance of genes involved in cardiovascular disease and cell communication were significantly different between captivity and wild breeding groups. The abundance of genes involved in the pathway of amino acid metabolism, carbohydrate metabolism, and lipid metabolism enriched in non-breeding groups compared with breeding groups. The results of neutral model revealed that captivity reduced the importance of stochastic processes in governing the bacterial community assembly, while breeding status increased the importance of stochastic processes in structuring the community assembly. Our results provided new insights on the effects of captivity on the microbial composition, diversity, function, and assembly processes and the interactions between microbiotas and physiological state of plateau pika, which provided a new theoretical basis to promote the possibility of successful reproduction in laboratory.
Key words： Gut microbe; Plateau pika; 16S rDNA gene; Structure and function; Assembly process
Liangzhi ZHANG , Xianjiang TANG , Shien Ren , Yaqi ZHAO , Yanming ZHANG . Effects of captivity on the assembly process of microbiota communities of plateau pikas[J]. ACTA THERIOLOGICA SINICA, 2022 , 42(5) : 519 -530 . DOI: 10.16829/j.slxb.150721
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