ACTA THERIOLOGICA SINICA >

2025 , Vol. 45 >Issue 2: 175 - 184

DOI: https://doi.org/10.16829/j.slxb.150956

ORIGINAL PAPERS

Characteristics of Hipposideros armiger armiger (Chiroptera: Hipposideridae) from Xizang, and a reevaluation of H. armiger subspecies taxonomy

  • SU Haohui ,
  • DIAO Haixin ,
  • ZHAN Xiaqin ,
  • HE Minyi ,
  • HUANG Zefeng ,
  • WU Yi ,
  • LIN Lianggong ,
  • CHEN Zhongzheng ,
  • JIANG Xuelong ,
  • WANG Xiaoyun ,
  • YU Wenhua
Expand
  • 1 Key Laboratory of Conservation and Application in Biodiversity of South China, School of Life Sciences, Guangzhou University, Guangzhou 510006, China;
    2 School of Ecology and Environment, Anhui Normal University, Wuhu 241001, China;
    3 Center for Ecology and Environment, Tunghai University, Taichung 40341, China;
    4 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650201, China

Received date: 2024-05-13

  Revised date: 2024-12-31

  Online published: 2025-04-01

Fold

Abstract

Hipposideros armiger (Chiroptera: Hipposideridae) is traditionally categorized into four recognized subspecies: H. a. armiger, H. a. fujianensis, H. a. terasensis, and H. a. traninhensis. In China, the first three subspecies are known to be widely distributed across 16 provinces. However, the validity of this subspecies classifacation is still debated. In order to reassess and clarify the subspecies classification status of H. armiger, based on samples recently collected from Xizang, which are geographically close to the type locality in Nepal, we integrated morphological and molecular data and evidences from samples from various provinces. Our findings indicate a lack of significant morphological and cranial metric differences among H. a. armiger, H. a. fujianensis, and H. a. terasensis. The phylogenetic analysis revealed that sequences from China forming a well-supported distinct lineage, with sequences from Fujian and Taiwan nested within this lineage. The genetic distances among the three subspecies are minimal, ranging from 0. 002 to 0. 013.The haplotype network analysis also reveals no significant population genetic structure or geographic differences among three subspecies. The morphological and molecular evidence presented herein supports the reclassification of H. armiger in China as a single subspecies, H. a. armiger. This results not only clarifies the subspecies classification of H. armiger in China, but also provides foundational data for future research on phylogeography and conservation status.

Key words： Chiroptera(bats); Hipposideros armiger(Greater leaf-nosed bat); Subspecies; Xizang

Cite this article

SU Haohui , DIAO Haixin , ZHAN Xiaqin , HE Minyi , HUANG Zefeng , WU Yi , LIN Lianggong , CHEN Zhongzheng , JIANG Xuelong , WANG Xiaoyun , YU Wenhua . Characteristics of Hipposideros armiger armiger (Chiroptera: Hipposideridae) from Xizang, and a reevaluation of H. armiger subspecies taxonomy[J]. ACTA THERIOLOGICA SINICA, 2025 , 45(2) : 175 -184 . DOI: 10.16829/j.slxb.150956

References

Bandelt H J, Forster P, Rohl A. 1999. Median-joining networks for in ferring intraspecific phylogenies. Molecular Biology and Evolu tion, 16:37-48.
Bates P J J, Harrison D L. 1997. Bats of the Indian Subcontinent.Kent:Harrison Zoological Museum, 204-205.
Bu Y Z, He X P, Zhou H X, Zhou H W, Zhang Z X, Zhang C, Niu H X. 2017. Morphology and mitochondrial gene divergence in Hip posideros armiger armiger occurs only in China. Mammalia, 81(2):135-146.
Chakravarty R, Ruedi M, Ishtiaq F. 2020. A recent survey of bats with descriptions of echolocation calls and new records from the west ern Himalayan Region of Uttarakhand, India. Acta Chiroptero logica, 22 (1):197-224.
Dawson A G. 1992. Ice Age Earth:Late Quaternary Geology and Cli mate. New York:Chapman and Hall Press, 320.
Drummond A J, Ashoton B, Buxton S, Cheung M. 2016. Geneious v5. 6[CP/OL].[2016-08-01]. http://www. geneious. com/.
Edgar R C. 2004. MUSCLE:multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5):1792-1797.Huang Z S, He X Y, Peng X W, Deng J, Chen C J, Liu P Y, Zhang L B. 2024. Hipposideros armiger found in Medog, Xizang, China.Chinese Journal of Zoology, 59 (5):781-788. (in Chinese)
Jiang Z G, Wu Y, Liu S Y, Jiang X L, Zhou K Y, Hu H J. 2020. China's Red List of Biodiversity:Vertebrates. Volume Ⅰ, Mammals. Bei jing:Science Press, 828-829. (in Chinese)
Jombart T, Devillard S, Balloux F. 2010. Discriminant analysis of principal components:a new method for the analysis of geneti cally structured populations. BMC Genetics, 11 (1):94.
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide se quences. Journal of Molecular Evolution, 16 (2):111-120.
Lin A Q, Csorba G, Li L F, Jiang T L, Lu G J, Thong V D, Soisook P, Sun K P, Feng J. 2013. Phylogeography of Hipposideros armiger(Chiroptera:Hipposideridae) in the Oriental Region:the contribu tion of multiple Pleistocene glacial refugia and intrinsic factors to contemporary population genetic structure. Journal of Biogeogra phy, 41:317-327.
Lin A Q, Sun K P, Feng J. 2014. Molecular phylogeography of bats in China. Acta Theriologica Sinica, 34 (3):298-306. (in Chinese)
Lin A Q. 2014. Geographical evolutionary divergence in acoustie sig nals of the great himalayan leaf-nosed bat. Ph. D thesis.Changchun:Northeast Normal University. (in Chinese)
Liu S Y, Wu Y, Li S. 2022. Handbook of the Mammals of China. 3rd ed. Fuzhou:Straits Books Press, 68. (in Chinese)
Lu G J. 2009. The geographic variation among the echolocation calls of Hipposideros armiger in different locations. Master thesis.Changchun:Northeast Normal University. (in Chinese)
Mayr E, Linsley E G, Usinger R L. 1953. Methods and Principles of Systematic Zoology. New York:McGraw-Hill Book Com pany, 317.
Nguyen L T, Schmidt H A, von Haeseler A, Minh B Q. 2015. IQTREE:a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolu tion, 32 (1):268-274.
Paradis E, Schliep K. 2019. ape 5. 0:an environment for modern phy logenetics and evolutionary analyses in R. Bioinformatics, 35:526-528.
Revelle W. 2020. Psych:procedures for personality and psychological research[CP/OL]. Evanston, Illinois, USA:Northwestern Univer sity[2020-09-04]. https://CRAN. R-project. org/package=psych Version=2. 0. 8.
Rozas J, Ferrer-Mata A, Sanchez-Delbarrio J C, Guirao-Rico S, Lib rado P, Ramos-Onsins S E, Sanchez G A. 2017. DnaSP 6:DNA sequence polymorphism analysis of large data sets. Molecular Bi ology and Evolution, 34 (12):3299-3302.
Sazali S N, Besar K, Abdullah M T. 2011. Phylogenetic analysis of the Malaysian Rhinolopus and Hipposideros inferred from partial mitochondrial DNA Cytochrome b gene sequences. Pertanika Journal of Tropical Agricultural Science, 34:281-294.
Smith A T, Xie Y. 2009. A Guide to the Mammals of China. Chang sha:Hunan Education Publishing House, 297. (in Chinese)
Tan B J. 1992. A Systematic List of the Mammals. Beijing:China Medical Science Press, 61-62. (in Chinese)
Thong V D, Puechmaille S J, Denzinger A, Dietz C, Csorba G, Bates P J J, Teeling E C, Schnitzler H U. 2012. A new species of Hippo sideros (Chiroptera:Hipposideridae) from Vietnam. Journal of Mammalogy, 93 (1):1-11.
Wang Y X. 2003. A Complete Checklist of Mammal Species and Sub species in China:A Taxonomic and Geographic Reference. Bei jing:China Forestry Publishing House, 37. (in Chinese)
Wei F W, Yang Q S, Wu Y, Jiang X L, Liu S Y. 2022. Taxonomy and Distribution of Mammals in China. Beijing:Science Press, 309-310. (in Chinese)
Wickham H. 2016. Ggplot2:Elegant Graphics for Data Analysis.New York:Springer-Verlag.
Wilson D E, Mittermeier R A. 2019. Handbook of the Mammals of the World. Volume 9:Bats. Barcelona:Lynx Edicions, 237-238.
Yang Q S, Xia L, Feng Z J, Ma Y, Quan G Q, Wu Y. 2007. A guide to the measurement of mammal skull V:Insectivora and Chiroptera.Chinese Journal of Zoology, 42 (2):56-62. (in Chinese)
You Y Y, Sun K P, Xu L J, Wang L, Jiang T L, Liu S, Lu G J, Sean B, Feng J. 2010. Pleistocene glacial cycle effects on the phylogeog raphy of the Chinese endemic bat species, Myotis davidii. BMC Evolutionary Biology, 10:208.
Yu W H, Chen Z, Li Y C, Wu Y. 2012. Phylogeographic relationships of Scotophilus kuhlii between Hainan Island and mainland of China. Mammal Study, 37:139-146.
Zhang C. 2014. Study on infraspecific classification and habitat selec tion of Hipposideros armiger. Master thesis. Xinxiang:Henan Normal University. (in Chinese)
Zhao H T, Zhang Q M, Song C J. 1999. Geomorphology and Environ ment of the South China Coast and the South China Sea Islands.Beijing:Science Press, 447.
Zheng X Y. 1987. A survey of the bats (Chiroptera) of Fujian Prov ince. Wuyi Science Journal, 7:237-242. (in Chinese)Smith A T, 解焱. 2009. 中国兽类野外手册. 长沙:湖南教育出版社, 297.
王应祥. 2003. 中国哺乳动物物种和亚种分类名录与分布大全. 北京:中国林业出版社, 37.
卢冠军. 2009. 大蹄蝠不同种群回声定位声波地理差异研究. 长春:东北师范大学硕士学位论文.
刘少英, 吴毅, 李晟. 2022. 中国兽类图鉴. 第 3 版. 福州:海峡书局出版社, 68.
张婵. 2014. 大蹄蝠的种下分类及栖息地选择研究. 新乡:河南师范大学硕士学位论文.
杨奇森, 夏霖, 冯祚建, 马勇, 全国强, 吴毅. 2007. 兽类头骨测量标准Ⅴ:食虫目、翼手目. 动物学杂志, 42 (2):56-62.
林爱青, 孙克萍, 冯江. 2014. 我国翼手类分子系统地理学研究进展. 兽类学报, 34 (3):298-306.
林爱青. 2014. 大蹄蝠声信号的地理进化. 长春:东北师范大学博士学位论文.
郑秀芸. 1987. 福建省翼手类调查初报. 武夷科学, 7:237-242.
黄泽双, 何向阳, 彭兴文, 邓瑾, 陈创杰, 刘佩莹, 张礼标. 2024. 西藏墨脱发现大蹄蝠. 动物学杂志, 59 (5):781-788.
蒋志刚, 吴毅, 刘少英, 蒋学龙, 周开亚, 胡慧建. 2021. 中国生物多样性红色名录:脊椎动物 第一卷 哺乳动物 (中册). 北京:科学出版社, 828-829.
谭邦杰. 1992. 哺乳动物分类名录. 北京:中国医药科技出版社, 61-62.
魏辅文, 杨奇森, 吴毅, 蒋学龙, 刘少英. 2022. 中国兽类分类与分布. 北京:科学出版社, 309-310.
Options
Outlines

/

〈 〉

　青公网安备 63010402000199号 青ICP备05000010号-2
Copyright © ACTA THERIOLOGICA SINICA, All Rights Reserved.
Tel: +86-971-6143617　Fax: +86-971-6143282　E-mail: slxb@nwipb.cas.cn
Powered by Beijing Magtech Co., Ltd.