Abstract: Hipposideros armiger (Chiroptera: Hipposideridae) is traditionally categorized into four recognized subspecies: H. a. armiger, H. a. fujianensis, H. a. terasensis, and H. a. traninhensis. In China, the first three subspecies are known to be widely distributed across 16 provinces. However, the validity of this subspecies classifacation is still debated. In order to reassess and clarify the subspecies classification status of H. armiger, based on samples recently collected from Xizang, which are geographically close to the type locality in Nepal, we integrated morphological and molecular data and evidences from samples from various provinces. Our findings indicate a lack of significant morphological and cranial metric differences among H. a. armiger, H. a. fujianensis, and H. a. terasensis. The phylogenetic analysis revealed that sequences from China forming a well-supported distinct lineage, with sequences from Fujian and Taiwan nested within this lineage. The genetic distances among the three subspecies are minimal, ranging from 0. 002 to 0. 013.The haplotype network analysis also reveals no significant population genetic structure or geographic differences among three subspecies. The morphological and molecular evidence presented herein supports the reclassification of H. armiger in China as a single subspecies, H. a. armiger. This results not only clarifies the subspecies classification of H. armiger in China, but also provides foundational data for future research on phylogeography and conservation status.

Key words: Chiroptera(bats), Hipposideros armiger(Greater leaf-nosed bat), Subspecies, Xizang

摘要： 大蹄蝠(Hipposideros armiger)隶属于翼手目蹄蝠科，目前划分指名亚种(H.a.armiger)、福建亚种(H.a.fujianensis)、台湾亚种(H.a.terasensis)和越南亚种(H.a.traninhensis)4个亚种，其中前3个亚种在我国广东、云南等16个省区广泛分布，但也有学者对其亚种的有效性存在争议。为进一步明确大蹄蝠的亚种分类状况，本研究以中国区域内最接近模式产地(尼泊尔)的西藏样本为切入点，结合历年采集于各省区的形态学数据和分子序列，对大蹄蝠的亚种分类进行探讨。结果显示，该种外形、头骨特征及测量数据均未发现指名亚种、福建亚种和台湾亚种间存在明显差异；系统发育树提示福建、台湾在内的多地大蹄蝠序列聚为高支持度的一支，未见明显分化，3个亚种间遗传距离仅0.002~0.013；单倍型网络图亦未发现各亚种间存在明显的种群遗传结构与地理差异。基于上述证据，本研究认为中国区域内的大蹄蝠应归为同一亚种——大蹄蝠指名亚种(H.a.armiger)，该结果不仅有助于进一步明确国内大蹄蝠的亚种分类地位，也为深入理解该种的演化历史提供了基础资料。

关键词: 翼手目(蝙蝠), 大蹄蝠, 亚种, 西藏