同一笼养生境下金头狮面狨猴、赤掌柽柳猴和棉顶狨猴肠道菌群比较

doi:10.16829/j.slxb.150739

摘要/Abstract

摘要： 动物的肠道菌群组成与宿主种类及其生存环境直接相关，研究同一生境下不同物种的肠道菌群组成对于物种多样性保护具有积极意义。本研究采用非损伤取样和16S rDNA高通量测序等方法，以南京市红山森林动物园笼养的金头狮面狨猴(Leontopithecus chrysomelas)、赤掌柽柳猴(Saguinus midas)和棉顶狨猴(Saguinus oedipus)三种新大陆猴为研究对象，比较分析其肠道菌群结构组成差异，并进行功能预测。结果发现：3个物种的肠道菌群合计14门20纲50目101科231属356种。优势菌门均为厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)，共有优势菌属为链球菌属(Streptococcus)和双歧杆菌属(Bifidobacterium)。3个物种间肠道菌群在门水平和属水平均存在显著差异，其中金头狮面狨猴的肠道菌群丰富度显著高于另外两个物种。本研究结果为灵长类肠道菌群多样性与生存适应研究提供基础数据，同时为相关物种的易地保护管理提供参考依据。

关键词: 新大陆猴, 肠道菌群, 高通量测序技术, 16S rDNA

Abstract: The composition of intestinal microbiota in animals is directly related to the host species and their living environment. Studies on intestinal microbiota composition of various species living in the same habitat is important for the conservation of species diversity. Both non-damage sampling and 16S rDNA high-throughput sequencing were used to investigate the structure, diversity, and functional prediction of intestinal microbiota across three species of New World monkeys (golden-headed lion tamarin Leontopithecus chrysomelas, golden-handed tamarin Saguinus midas, and cottonheaded tamarin Saguinus oedipus) from Nanjing Hongshan Forest Zoo. The results showed that a total of 14 phyla, 20 classes, 50 orders, 101 families, 231 genera, and 356 species of intestinal flora were obtained from 18 fecal samples collected from the three species. At the phylum level, the common dominant phyla across the three species were Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. At the genus level, the common dominant bacterial genera included Streptococcus and Bifidobacterium. There were significant differences in intestinal microbiota at both the phylum level and the genus level among species, in which the richness of intestinal microbiota in golden-headed lion tamarin was significantly higher than that in the others. This study provides basic data for further investigation on the intestinal microbiota diversity and survival adaptation, and also serves as reference for ex situ management for species conservation.

Key words: New world monkeys, Intestinal microbiota, High-throughput sequencing, 16S rDNA

中图分类号:

Q939

张泽明, 姚红玉, 吴楠, 赵玲玲, 顾逸如, 王宇, 吴弘, 赵大鹏. 同一笼养生境下金头狮面狨猴、赤掌柽柳猴和棉顶狨猴肠道菌群比较[J]. 兽类学报, 2023, 43(5): 580-592.

ZHANG Zeming, YAO Hongyu, WU Nan, ZHAO Lingling, GU Yiru, WANG Yu, WU Hong, ZHAO Dapeng. Comparison of intestinal microbiota of golden-headed lion tamarins, golden-handed tamarins and cotton-headed tamarins under the same captive environment[J]. ACTA THERIOLOGICA SINICA, 2023, 43(5): 580-592.

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[2]	王德华, 赵志军, 张学英, 张志强, 徐德立, 邢昕, 杨生妹, 王政昆, 高云芳, 杨明. 中国哺乳动物生理生态学研究进展与展望[J]. 兽类学报, 2021, 41(5): 537-555.
[3]	杨雄威, 彭彩淳, 郭群毅, 冉景丞, 王野影, 张明明, 胡灿实, 李仕泽, 粟海军. 贵州苗岭地区野猪肠道菌群结构与功能分析[J]. 兽类学报, 2021, 41(4): 365-376.
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