兽类学报

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利用微卫星研究普洱亚洲象种群的遗传多样性

杜娇娇 何长欢 朱頔 杨帆 张立   

  1. 北京师范大学生命科学学院
  • 出版日期:2017-04-20 发布日期:2017-04-20

Genetic diversity analysis based microsatellite of Asian Elephants in Puer

DU Jiaojiao, HE Changhuan, ZHU Di, YANG Fan, ZHANG Li   


  1. College of Life Sciences,Beijing Normal University
  • Online:2017-04-20 Published:2017-04-20

摘要: 本文采用非损伤性取样法获得普洱地区亚洲象粪便样品113份,试剂盒法提取粪便基因组总DNA,利用9对微卫星引物对DNA进行特异性扩增,CERVUS 3.0软件基因分型得到49个独特的基因型。利用GenAlEx v6.5与Arlequin v3.5进行位点遗传信息检测和种群遗传多样性分析,结果表明:研究采用的所有微卫星位点均未偏离哈迪温伯格平衡,位点的平均等位基因数为3.111,平均香农信息指数为0.804,平均期望杂合度为0.468,平均观测杂合度为0.476。比较相同位点上不同地区亚洲象的遗传杂合度,表明普洱地区亚洲象的种群遗传多样性较低。

关键词: 普洱, 亚洲象, 微卫星, 遗传多样性

Abstract:

Using a non-invasive method, 113 fecal samples from the Asian elephant population in Puer were collected. After getting total DNA using kits, specific amplification by 9 microsatellite loci resulted in a 49 unique genotypes which were identified by CERVUS 3.0. The analysis of genetic diversity through GenAlEx v6.5 and Arlequin v3.5 showed that loci didid not deviate from Hardy-Weinberg equilibrium; the average allele number was 3.111; the average Shannon's Information Index was 0.804; the average expected heterozygosity was 0.468and the average observed heterogosity was 0.476. Comparisons of genetic heterozygosity from different areas showed that the genetic diversity of Asian elephant in Puer was low.

 

Key words: Asian elephant, Genetic diversity, Microsatellite, Puer