兽类学报

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致赤大袋鼠皮肤化脓的荚膜血清A型多杀性巴氏杆菌的分离鉴定

周伟 李沐 王海军 祝令伟 孙洋 郭学军   

  1. 军事医学科学院军事兽医研究所;吉林省人兽共患病预防与控制重点实验室,吉林 长春 130122
  • 出版日期:2016-02-13 发布日期:2016-05-20
  • 通讯作者: 郭学军,E-mail:xuejung@yahoo.com
  • 作者简介:周伟( 1986-),男,硕士,研究实习员,主要从事分子细菌学研究.
  • 基金资助:
    国家”863”课题,项目编号:2012AA022006

Isolation and identification of a capsular serotype-A Pasteurella multocida that caused suppuration in Macropus rufus

ZHOU Wei,LI Mu,WANG Haijun,ZHU Lingwei,SUN Yang,GUO Xuejun   

  1. Institute of Military Veterinary,AMMS;Key laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122,China
  • Online:2016-02-13 Published:2016-05-20

关键词: 赤大袋鼠, 多杀性巴氏杆菌, 荚膜血清A型

Abstract: To find out the etiological cause of pyogenic infection of two Macropus rufus, three samples from the pus of the infected sites were collected and subject to microbiological examinations. A strain of gram-negative bacteria was isolated. Based on the results of the morphological and biochemical tests, multiple genes sequence analysis and ERIC-PCR fingerprints,the isolate was identified as a capsular serotype-A Pasteurella multocida and was then named as PM-1.PM-1 was lethal to mice with an median lethal dose (LD50) at 1.99×102 cfu/mice. It was found to be sensitive to levofloxacin and ciprofloxacin.Treatment with levofloxacin hydrochloride ointment therefore cured the infected sites in the animals and the spread of the disease was then under the control.

Key words: Capsular serotype-A, Macropus rufus, Pasteurella multocida