关键词: 马麝, 细胞色素b , 地理分化

Abstract: Alpine musk deer ( Moschus sifanicusg) is an important economic animal in China. Because of being over-hunting and environmental deterioration , populations of alpine musk deer have become isolated in their distributional area. In order to protect biodiversity , this study inquired about intra-specific genetic diversity among three populations of alpine musk deer (Sichuan , Ganshu , and Xizang) by molecular biological method. 352 bp of mitochondrial cytochrome b gene were sequenced by PCR , using a pair of universal primer (L14724 and H15149) , phylogenetic trees were contracted using maximum parsimony method and neighbor-joining method. The results suggested that these three populations of alpine musk deer may be different , sequence divergenece of cytb was 0.57 % - 2.84 % among the three populations , which was within level of intraspecies. The phylogenetic analysis revealed that they shared a common ancestor. Mega-years ago and the Sichuan population first diverged from the common ancestor. The results also revealed that the Gansu population was close to the Xizang populations ; the Gansu population diverged from the Sichuan population 0.8 Mega-years ago , and the Xizang populations diverged from Gansu population and Sichuan population 0.34 - 0.57 and 0.9 - 1 Mega-years ago. Results implied that these populations in Gansu , Sichuan and Xizang may be different subspecies.

Key words: Musk deer ( Moschus sifanicus), Cytochrome b, Geographical division