关键词: 甘肃鼢鼠, 低氧, 心脏, 抗氧化酶, ATP酶, 丙二醛

Abstract: The purpose of this study was to investigate the mechanisms of anti-oxidative damages and electrophysiological disorder during low oxygen stress in Myospalax cansus. Myospalax cansus and Rattus norvegicus were stressed under low oxygen (4. 5% )conditions for 2 h,4 h,6 h,8 h,10 h,or 16 h. The activities of three heart antioxidases including superoxide dismutase (SOD),catalase (CAT)and glutathione reductase (GR)and three ATPases including Ca^{2 +} - ATPase, Ca^{2 +}- Mg^{2 +} - ATPase and Na +- K ⁺ - ATPase were measured at each time point. Malondialdehyde (MDA)was used as indicator of oxidative damage. The results are as follows:under normal oxygen conditions,there was no significant difference in SOD,CAT,Ca^{2 +} - ATPase,Ca^{2 +}- Mg^{2 +} - ATPase,Na ⁺ - K⁺ - ATPase activities and MDA content between Myospalax cansus and Rattus norvegicus,but GR activity in Myospalax cansus was significantly higher than that in Rattus norvegicus. Under low oxygen conditions,the activities of all of the three heart antioxidases and the three ATPases in Myospalax cansus increased rapidly,and they were significantly higher than those in Rattus norvegicus,but Myospalax cansus had significantly lower MDA content than did Rattus norvegicus. We conclude that Myospalax cansus can maintain normal cardiac electrical activity,heart rate and good heart condition through increasing the activity of antioxidase,removing excessive free radicals induced by low oxygen condition and improving ATPase activity

Key words: Antioxidase, ATPase, Heart, Low oxygen condition, MDA, Myospalax cansus